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RNA-seq between asexual archeospores and meiosis-related conchospores in Neopyropia yezoensis using Smart-seq2
He, Bangxiang1,2,3; Gu, Wenhui1,2; Wang, Lijun1,2; Zheng, Zhenbing1,2; Shao, Zhizhuo1,2; Huan, Li1,2; Zhang, Baoyu1,2; Ma, Yingchao1,2; Niu, Jianfeng1,2; Xie, Xiujun1,2; Wang, Guangce1,2
2021-08-04
Source PublicationJOURNAL OF PHYCOLOGY
ISSN0022-3646
Pages11
Corresponding AuthorXie, Xiujun(xjxie@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
AbstractIn the life cycle of Neopyropia yezoensis, a potential model system for marine macroalgae, both asexual archeospores and meiosis-related conchospores develop into thalli (gametophyte). To understand this special life phenomenon in macroalgae, we picked out the two kinds of spores (10-30 cells in each sample) and conducted RNA-seq using Smart-seq2. Comparative analysis showed that light capture and carbon fixation associated differentially expressed genes (DEGs) were upregulated in archeospores, thus indicating that archeospores are in a state of rapid vegetative growth. In conchospores, protein synthesis and degradation, especially molecular chaperone, associated DEGs were up-regulated, indicating that complex life activities might be occurring in conchospores. There were 68 genes related to DNA replication and repair expressed in conchospores, showing that active DNA replication might occur in conchospores. Moreover, we found that one conchospore specifically expressed DEG (py04595: DNA helicase) only in diploid stages (conchocelis, sporangial filament) and three archeospores specifically expressed DEGs only in haploid stages (thalli). These molecular level results indicated that conchospores were closer to diploid, and might be the meiotic mother cells of N. yezoensis. In addition, we found that the knotted-like homeobox gene (PyKNOX), which might relate to the transition of gametophyte from sporophyte, was only expressed in sporophyte generation but not expressed in conchospores, archeospores and thalli, indicating the morphogenesis of gametophyte sin N. yezoensis might require the inactivation of PyKNOX.
Keywordarcheospores asexual reproduction conchospores meiosis RNA-seq Smart-seq2
DOI10.1111/jpy.13197
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41876160] ; National Natural Science Foundation of China[41776150] ; National Natural Science Foundation of China[41906105] ; National Natural Science Foundation of China[41876163] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science, the Chinese Academy of Sciences[COMS2019Q02] ; Major Scientific and Technological Innovation Projects in Shandong Province[2019JZZY010815] ; North Jiangsu Science and Technology Special Project[LYG-SZ201916] ; Science and Technology Project of Dongtou District[N2016Y18A]
WOS Research AreaPlant Sciences ; Marine & Freshwater Biology
WOS SubjectPlant Sciences ; Marine & Freshwater Biology
WOS IDWOS:000680918000001
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/175945
Collection实验海洋生物学重点实验室
Corresponding AuthorXie, Xiujun; Wang, Guangce
Affiliation1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
He, Bangxiang,Gu, Wenhui,Wang, Lijun,et al. RNA-seq between asexual archeospores and meiosis-related conchospores in Neopyropia yezoensis using Smart-seq2[J]. JOURNAL OF PHYCOLOGY,2021:11.
APA He, Bangxiang.,Gu, Wenhui.,Wang, Lijun.,Zheng, Zhenbing.,Shao, Zhizhuo.,...&Wang, Guangce.(2021).RNA-seq between asexual archeospores and meiosis-related conchospores in Neopyropia yezoensis using Smart-seq2.JOURNAL OF PHYCOLOGY,11.
MLA He, Bangxiang,et al."RNA-seq between asexual archeospores and meiosis-related conchospores in Neopyropia yezoensis using Smart-seq2".JOURNAL OF PHYCOLOGY (2021):11.
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