Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| RNA-seq between asexual archeospores and meiosis-related conchospores in Neopyropia yezoensis using Smart-seq2 | |
He, Bangxiang1,2,3; Gu, Wenhui1,2; Wang, Lijun1,2; Zheng, Zhenbing1,2; Shao, Zhizhuo1,2; Huan, Li1,2 ; Zhang, Baoyu1,2; Ma, Yingchao1,2; Niu, Jianfeng1,2; Xie, Xiujun1,2; Wang, Guangce1,2
| |
| 2021-08-04 | |
| Source Publication | JOURNAL OF PHYCOLOGY
 |
| ISSN | 0022-3646 |
| Pages | 11 |
| Corresponding Author | Xie, Xiujun(xjxie@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn) |
| Abstract | In the life cycle of Neopyropia yezoensis, a potential model system for marine macroalgae, both asexual archeospores and meiosis-related conchospores develop into thalli (gametophyte). To understand this special life phenomenon in macroalgae, we picked out the two kinds of spores (10-30 cells in each sample) and conducted RNA-seq using Smart-seq2. Comparative analysis showed that light capture and carbon fixation associated differentially expressed genes (DEGs) were upregulated in archeospores, thus indicating that archeospores are in a state of rapid vegetative growth. In conchospores, protein synthesis and degradation, especially molecular chaperone, associated DEGs were up-regulated, indicating that complex life activities might be occurring in conchospores. There were 68 genes related to DNA replication and repair expressed in conchospores, showing that active DNA replication might occur in conchospores. Moreover, we found that one conchospore specifically expressed DEG (py04595: DNA helicase) only in diploid stages (conchocelis, sporangial filament) and three archeospores specifically expressed DEGs only in haploid stages (thalli). These molecular level results indicated that conchospores were closer to diploid, and might be the meiotic mother cells of N. yezoensis. In addition, we found that the knotted-like homeobox gene (PyKNOX), which might relate to the transition of gametophyte from sporophyte, was only expressed in sporophyte generation but not expressed in conchospores, archeospores and thalli, indicating the morphogenesis of gametophyte sin N. yezoensis might require the inactivation of PyKNOX. |
| Keyword | archeospores asexual reproduction conchospores meiosis RNA-seq Smart-seq2 |
| DOI | 10.1111/jpy.13197 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key R&D Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41876160] ; National Natural Science Foundation of China[41776150] ; National Natural Science Foundation of China[41906105] ; National Natural Science Foundation of China[41876163] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science, the Chinese Academy of Sciences[COMS2019Q02] ; Major Scientific and Technological Innovation Projects in Shandong Province[2019JZZY010815] ; North Jiangsu Science and Technology Special Project[LYG-SZ201916] ; Science and Technology Project of Dongtou District[N2016Y18A] |
| WOS Research Area | Plant Sciences ; Marine & Freshwater Biology |
| WOS Subject | Plant Sciences ; Marine & Freshwater Biology |
| WOS ID | WOS:000680918000001 |
| Publisher | WILEY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/175945 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Xie, Xiujun; Wang, Guangce |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | He, Bangxiang,Gu, Wenhui,Wang, Lijun,et al. RNA-seq between asexual archeospores and meiosis-related conchospores in Neopyropia yezoensis using Smart-seq2[J]. JOURNAL OF PHYCOLOGY,2021:11. |
| APA | He, Bangxiang.,Gu, Wenhui.,Wang, Lijun.,Zheng, Zhenbing.,Shao, Zhizhuo.,...&Wang, Guangce.(2021).RNA-seq between asexual archeospores and meiosis-related conchospores in Neopyropia yezoensis using Smart-seq2.JOURNAL OF PHYCOLOGY,11. |
| MLA | He, Bangxiang,et al."RNA-seq between asexual archeospores and meiosis-related conchospores in Neopyropia yezoensis using Smart-seq2".JOURNAL OF PHYCOLOGY (2021):11. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| jpy.13197.pdf（493KB） | 期刊论文 | 出版稿 | 延迟开放 | CC BY-NC-SA | View 2023-7-1后可获取 | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment