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The Characteristic of Critical Genes in Neuroendocrine System and Their Regulation on Food Habit Transition and Metamorphosis of Veined Rapa Whelk Rapana venosa (Valenciennes, 1846)
Yang, Mei-Jie1,2,3,4,5; Feng, Jie1,2,3,4; Song, Hao1,2,3,4; Yu, Zheng-Lin1,2,3,4; Shi, Pu1,2,3,4,5; Liang, Jian6; Hu, Zhi1,2,3,4,5; Zhou, Cong1,2,3,4,5; Wang, Xiao-Lin1,2,3,4; Zhang, Tao1,2,3,4
2021-07-16
Source PublicationFRONTIERS IN MARINE SCIENCE
Volume8Pages:14
Corresponding AuthorZhang, Tao(tzhang@qdio.ac.cn)
AbstractMetamorphosis is a critical developmental event in mollusks, and neuroendocrine system plays an essential role in this process. Rapana venosa is an economically important shellfish in China, but the artificial technology of R. venosa aquaculture is limited by metamorphosis. As a carnivorous gastropod, food habit transition makes the mechanism of R. venosa metamorphosis more complex. To investigate the changes in the neuroendocrine system and to reveal its role in regulating the food habit transition and metamorphosis of R. venosa, we cloned the cDNA sequences encoding 5-hydroxytryptamine receptor (Rv-5HTR), nitric oxide synthetase (Rv-NOS) and cholecystokinin receptor (Rv-CCKR), and investigated their expression by quantitative real-time PCR analysis, and explore the spatio-temporal changes of 5-HT protein expression using Immunohistochemical (IHC) analysis. The expression of the three geens was significantly increased in the early intramembrane veliger stage, which indicates that the three genes are related to the development of digestive system. Additionally, expression of the three genes was decreased after metamorphosis, while Rv-NOS and Rv-CCKR were increasingly expressed in competent larvae, which may help the larvae find suitable environments and promote digestive system development for metamorphosis, and the result of 5-HT IHC analysis also reflects the development of neuroendocrine system. Furthermore, results show that CCK can effect the expression of digestive enzyme, NOS and 5-HT receptor. Finally, based on the present results, we hypothesized that CCK and CCK receptor may be critical regulatory factors of food habit transition and metamorphosis. These results might provide information on the development of neuroendocrine system of R. venosa, and new insight into the regulation of the food habit transition and metamorphosis of gastropods.
KeywordRapana venosa metamorphosis neuroendocrine system food habit transition digestive system
DOI10.3389/fmars.2021.690282
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31972814] ; National Natural Science Foundation of China[32002409] ; Natural Science Foundation of Shandong Province[ZR2019BD003] ; China Postdoctoral Science Foundation[2019M652498] ; Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program[2019JZZY020708] ; Earmarked Fund for Modern Agro-Industry Technology Research System[CARS-49] ; Industry Leading Talents Project of Taishan Scholars[LJNY201704] ; Double Hundred Blue Industry Leader Team of Yantai ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEESCTSP2018-1]
WOS Research AreaEnvironmental Sciences & Ecology ; Marine & Freshwater Biology
WOS SubjectEnvironmental Sciences ; Marine & Freshwater Biology
WOS IDWOS:000679892100001
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/175917
Collection海洋生态与环境科学重点实验室
Corresponding AuthorZhang, Tao
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Qingdao, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao, Peoples R China
5.Univ Chinese Acad Sci, Beijing, Peoples R China
6.Tianjin Agr Univ, Fisheries Coll, Tianjin Key Lab Aquaecol & Aquaculture, Tianjin, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang, Mei-Jie,Feng, Jie,Song, Hao,et al. The Characteristic of Critical Genes in Neuroendocrine System and Their Regulation on Food Habit Transition and Metamorphosis of Veined Rapa Whelk Rapana venosa (Valenciennes, 1846)[J]. FRONTIERS IN MARINE SCIENCE,2021,8:14.
APA Yang, Mei-Jie.,Feng, Jie.,Song, Hao.,Yu, Zheng-Lin.,Shi, Pu.,...&Zhang, Tao.(2021).The Characteristic of Critical Genes in Neuroendocrine System and Their Regulation on Food Habit Transition and Metamorphosis of Veined Rapa Whelk Rapana venosa (Valenciennes, 1846).FRONTIERS IN MARINE SCIENCE,8,14.
MLA Yang, Mei-Jie,et al."The Characteristic of Critical Genes in Neuroendocrine System and Their Regulation on Food Habit Transition and Metamorphosis of Veined Rapa Whelk Rapana venosa (Valenciennes, 1846)".FRONTIERS IN MARINE SCIENCE 8(2021):14.
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