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Probing the Energetic Metabolism of Resting Cysts under Different Conditions from Molecular and Physiological Perspectives in the Harmful Algal Blooms-Forming Dinoflagellate Scrippsiella trochoidea
Li, Fengting1,2; Yang, Aoao1; Hu, Zhangxi1,3,4; Lin, Siheng1; Deng, Yunyan1,3,4; Tang, Ying Zhong1,3,4
2021-07-01
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume22Issue:14Pages:17
Corresponding AuthorDeng, Yunyan(yunyandeng@qdio.ac.cn) ; Tang, Ying Zhong(yingzhong.tang@qdio.ac.cn)
AbstractEnergetic metabolism is essential in maintaining the viability of all organisms. Resting cysts play important roles in the ecology of dinoflagellates, particularly for harmful algal blooms (HABs)-causative species. However, the energetic metabolism underlying the germination potency maintenance of resting cysts of dinoflagellate have been extremely scarce in studies from physiological and, particularly, molecular perspectives. Therefore, we used the cosmopolitan Scrippsiella trochoidea as a representative of HABs-forming and cyst-producing dinoflagellates in this work to obtain novel insights into the molecular mechanisms, regulating the energetic metabolism in dinoflagellate resting cysts, under different physical condition. As the starting step, we established a cDNA subtractive library via suppression subtractive hybridization (SSH) technology, from which we screened an incomplete sequence for the beta subunit of ATP synthase gene (beta-F-1-ATPase), a key indicator for the status of cell's energetic metabolism. The full-length cDNA of beta-F-1-ATPase gene from S.trochoidea (St beta-F-1-ATPase) was then obtained via rapid amplification of cDNA ends (RACE) (Accession: MZ343333). Our real-time qPCR detections, in vegetative cells and resting cysts treated with different physical conditions, revealed that (1) the expression of St beta-F 1 -ATPase in resting cysts was generally much lower than that in vegetative cells, and (2) the St beta-F-1-ATPase expressions in the resting cysts under darkness, lowered temperature, and anoxia, and during an extended duration of dormancy, were significantly lower than that in cysts under the condition normally used for culture-maintaining (a 12 h light:12 h dark cycle, 21 degrees C, aerobic, and newly harvested). Our detections of the viability (via Neutral Red staining) and cellular ATP content of resting cysts, at the conditions corresponding to the abovementioned treatments, showed that both the viability and ATP content decreased rapidly within 12 h and then maintained at low levels within the 4-day experimentation under all the three conditions applied (4 degrees C, darkness, and anoxia), which are well in accordance with the measurements of the transcription of St beta-F-1-ATPase. These results demonstrated that the energy consumption of resting cysts reaches a low, but somehow stable, level within a short time period and is lower at low temperature, darkness, and anoxia than that at ambient temperature. Our work provides an important basis for explaining that resting cysts survive long-term darkness and low temperature in marine sediments from molecular and physiological levels.
KeywordATP content ATP synthase subunit beta (beta-F-1-ATPase) energetic metabolism resting cyst Scrippsiella trochoidea suppression subtractive hybridization (SSH) viability
DOI10.3390/ijms22147325
Indexed BySCI
Language英语
Funding ProjectKey Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2019Q09] ; National Science Foundation of China[41606126] ; National Science Foundation of China[41776125]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000677344800001
PublisherMDPI
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/175814
Collection海洋生态与环境科学重点实验室
Corresponding AuthorDeng, Yunyan; Tang, Ying Zhong
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS
Corresponding Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Fengting,Yang, Aoao,Hu, Zhangxi,et al. Probing the Energetic Metabolism of Resting Cysts under Different Conditions from Molecular and Physiological Perspectives in the Harmful Algal Blooms-Forming Dinoflagellate Scrippsiella trochoidea[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(14):17.
APA Li, Fengting,Yang, Aoao,Hu, Zhangxi,Lin, Siheng,Deng, Yunyan,&Tang, Ying Zhong.(2021).Probing the Energetic Metabolism of Resting Cysts under Different Conditions from Molecular and Physiological Perspectives in the Harmful Algal Blooms-Forming Dinoflagellate Scrippsiella trochoidea.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(14),17.
MLA Li, Fengting,et al."Probing the Energetic Metabolism of Resting Cysts under Different Conditions from Molecular and Physiological Perspectives in the Harmful Algal Blooms-Forming Dinoflagellate Scrippsiella trochoidea".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.14(2021):17.
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