Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Structural characteristics and immune-enhancing activity of an extracellular polysaccharide produced by marine Halomonas sp. 2E1 | |
Wang, QingChi1,2,3,4 ; Wei, Maosheng1,2,3,4; Zhang, Jingjing5; Yue, Yang1,2,3,4; Wu, Ning1,2,3,4; Geng, Lihua1,2,3,4; Sun, Chaomin1,2,3,4; Zhang, Quanbin1,2,3,4; Wang, Jing1,2,3,4
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| 2021-07-31 | |
| Source Publication | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| ISSN | 0141-8130 |
| Volume | 183Pages:1660-1668 |
| Corresponding Author | Zhang, Quanbin(qbzhang@qdio.ac.cn) ; Wang, Jing(jingwang@qdio.ac.cn) |
| Abstract | Microbial polysaccharides from extreme environments, such as cold seeps and hydrothermal vents, usually exhibit novel structural features and diverse biological activities. In this study, an exopolysaccharide (EPS2E1) was isolated from cold-seep bacterium Halomonas sp. 2E1 and its immune-enhancing activity was evaluated. The total sugar content and protein content were determined as 83.1% and 7.9%, respectively. EPS2E1 contained mannose and glucose with the molar ratio of 3.76: 1. The molecular weight was determined to be 47.0 kDa. Structural analysis indicated that EPS2E1 was highly branched, the backbone mainly consisted of -> 2)-Man-(alpha-1 -> and -> 2, 6)-Man-(alpha-1 -> with the ratio of 2.45: 1.00. The chain also contained -> 4)-Glc-(alpha-1 ->, -> 6)-Man-(alpha-1 -> and -> 3)-Glc-(beta-1 ->. EPS2E1 could significantly increase the production of NO, COX-2, TNF-alpha, IL-1 beta and IL-6 by activating the MAPK and NF-KB pathways on RAW264.7 macrophages. EPS2E1 exhibits the potential to be an immunopotentiator in the near future. (c) 2021 Elsevier B.V. All rights reserved. |
| Keyword | Halomonas sp Extracellular polysaccharide Structure Immune-enhancing activity Macrophages |
| DOI | 10.1016/j.ijbiomac.2021.05.143 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Special Open Fund of Laboratory for Marine Biology and Biotechnology, Qingdao Pilot National Laboratory for Marine Science and Technology[OF2020NO02] ; China Ocean Mineral Resources RD Association[DY135-B2-14] ; National Key research and Development Program of China[2019YFD0900705] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
| WOS ID | WOS:000675833200006 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/175780 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Zhang, Quanbin; Wang, Jing |
| Affiliation | 1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Qingdao Eighth Peoples Hosp, Pharmaceut Dept, Qingdao 266000, Peoples R China |
| First Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Corresponding Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Wang, QingChi,Wei, Maosheng,Zhang, Jingjing,et al. Structural characteristics and immune-enhancing activity of an extracellular polysaccharide produced by marine Halomonas sp. 2E1[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,183:1660-1668. |
| APA | Wang, QingChi.,Wei, Maosheng.,Zhang, Jingjing.,Yue, Yang.,Wu, Ning.,...&Wang, Jing.(2021).Structural characteristics and immune-enhancing activity of an extracellular polysaccharide produced by marine Halomonas sp. 2E1.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,183,1660-1668. |
| MLA | Wang, QingChi,et al."Structural characteristics and immune-enhancing activity of an extracellular polysaccharide produced by marine Halomonas sp. 2E1".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 183(2021):1660-1668. |
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| File Name/Size | DocType | Version | Access | License | ||
| 1-s2.0-S014181302101（2216KB） | 期刊论文 | 出版稿 | 延迟开放 | CC BY-NC-SA | View 2023-7-1后可获取 | |
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