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Structural characteristics and immune-enhancing activity of an extracellular polysaccharide produced by marine Halomonas sp. 2E1
Wang, QingChi1,2,3,4; Wei, Maosheng1,2,3,4; Zhang, Jingjing5; Yue, Yang1,2,3,4; Wu, Ning1,2,3,4; Geng, Lihua1,2,3,4; Sun, Chaomin1,2,3,4; Zhang, Quanbin1,2,3,4; Wang, Jing1,2,3,4
2021-07-31
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume183Pages:1660-1668
Corresponding AuthorZhang, Quanbin(qbzhang@qdio.ac.cn) ; Wang, Jing(jingwang@qdio.ac.cn)
AbstractMicrobial polysaccharides from extreme environments, such as cold seeps and hydrothermal vents, usually exhibit novel structural features and diverse biological activities. In this study, an exopolysaccharide (EPS2E1) was isolated from cold-seep bacterium Halomonas sp. 2E1 and its immune-enhancing activity was evaluated. The total sugar content and protein content were determined as 83.1% and 7.9%, respectively. EPS2E1 contained mannose and glucose with the molar ratio of 3.76: 1. The molecular weight was determined to be 47.0 kDa. Structural analysis indicated that EPS2E1 was highly branched, the backbone mainly consisted of -> 2)-Man-(alpha-1 -> and -> 2, 6)-Man-(alpha-1 -> with the ratio of 2.45: 1.00. The chain also contained -> 4)-Glc-(alpha-1 ->, -> 6)-Man-(alpha-1 -> and -> 3)-Glc-(beta-1 ->. EPS2E1 could significantly increase the production of NO, COX-2, TNF-alpha, IL-1 beta and IL-6 by activating the MAPK and NF-KB pathways on RAW264.7 macrophages. EPS2E1 exhibits the potential to be an immunopotentiator in the near future. (c) 2021 Elsevier B.V. All rights reserved.
KeywordHalomonas sp Extracellular polysaccharide Structure Immune-enhancing activity Macrophages
DOI10.1016/j.ijbiomac.2021.05.143
Indexed BySCI
Language英语
Funding ProjectSpecial Open Fund of Laboratory for Marine Biology and Biotechnology, Qingdao Pilot National Laboratory for Marine Science and Technology[OF2020NO02] ; China Ocean Mineral Resources RD Association[DY135-B2-14] ; National Key research and Development Program of China[2019YFD0900705]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000675833200006
PublisherELSEVIER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/175780
Collection实验海洋生物学重点实验室
Corresponding AuthorZhang, Quanbin; Wang, Jing
Affiliation1.Chinese Acad Sci, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Qingdao Eighth Peoples Hosp, Pharmaceut Dept, Qingdao 266000, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, QingChi,Wei, Maosheng,Zhang, Jingjing,et al. Structural characteristics and immune-enhancing activity of an extracellular polysaccharide produced by marine Halomonas sp. 2E1[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,183:1660-1668.
APA Wang, QingChi.,Wei, Maosheng.,Zhang, Jingjing.,Yue, Yang.,Wu, Ning.,...&Wang, Jing.(2021).Structural characteristics and immune-enhancing activity of an extracellular polysaccharide produced by marine Halomonas sp. 2E1.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,183,1660-1668.
MLA Wang, QingChi,et al."Structural characteristics and immune-enhancing activity of an extracellular polysaccharide produced by marine Halomonas sp. 2E1".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 183(2021):1660-1668.
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