Structural characteristics and immune-enhancing activity of an extracellular polysaccharide produced by marine Halomonas sp. 2E1

doi:10.1016/j.ijbiomac.2021.05.143

IOCAS-IR > 实验海洋生物学重点实验室

	Structural characteristics and immune-enhancing activity of an extracellular polysaccharide produced by marine Halomonas sp. 2E1
	Wang, QingChi1,2,3,4 ; Wei, Maosheng 1,2,3,4; Zhang, Jingjing 5; Yue, Yang1,2,3,4 ; Wu, Ning 1,2,3,4; Geng, Lihua 1,2,3,4; Sun, Chaomin 1,2,3,4; Zhang, Quanbin 1,2,3,4; Wang, Jing1,2,3,4
	2021-07-31
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	183 页码:1660-1668
通讯作者	Zhang, Quanbin(qbzhang@qdio.ac.cn) ; Wang, Jing(jingwang@qdio.ac.cn)
摘要	Microbial polysaccharides from extreme environments, such as cold seeps and hydrothermal vents, usually exhibit novel structural features and diverse biological activities. In this study, an exopolysaccharide (EPS2E1) was isolated from cold-seep bacterium Halomonas sp. 2E1 and its immune-enhancing activity was evaluated. The total sugar content and protein content were determined as 83.1% and 7.9%, respectively. EPS2E1 contained mannose and glucose with the molar ratio of 3.76: 1. The molecular weight was determined to be 47.0 kDa. Structural analysis indicated that EPS2E1 was highly branched, the backbone mainly consisted of -> 2)-Man-(alpha-1 -> and -> 2, 6)-Man-(alpha-1 -> with the ratio of 2.45: 1.00. The chain also contained -> 4)-Glc-(alpha-1 ->, -> 6)-Man-(alpha-1 -> and -> 3)-Glc-(beta-1 ->. EPS2E1 could significantly increase the production of NO, COX-2, TNF-alpha, IL-1 beta and IL-6 by activating the MAPK and NF-KB pathways on RAW264.7 macrophages. EPS2E1 exhibits the potential to be an immunopotentiator in the near future. (c) 2021 Elsevier B.V. All rights reserved.
关键词	Halomonas sp Extracellular polysaccharide Structure Immune-enhancing activity Macrophages
DOI	10.1016/j.ijbiomac.2021.05.143
收录类别	SCI
语种	英语
资助项目	Special Open Fund of Laboratory for Marine Biology and Biotechnology, Qingdao Pilot National Laboratory for Marine Science and Technology[OF2020NO02] ; China Ocean Mineral Resources RD Association[DY135-B2-14] ; National Key research and Development Program of China[2019YFD0900705]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:000675833200006
出版者	ELSEVIER
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/175780
专题	实验海洋生物学重点实验室
通讯作者	Zhang, Quanbin; Wang, Jing
作者单位	1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Qingdao Eighth Peoples Hosp, Pharmaceut Dept, Qingdao 266000, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Wang, QingChi,Wei, Maosheng,Zhang, Jingjing,et al. Structural characteristics and immune-enhancing activity of an extracellular polysaccharide produced by marine Halomonas sp. 2E1[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,183:1660-1668.
APA	Wang, QingChi.,Wei, Maosheng.,Zhang, Jingjing.,Yue, Yang.,Wu, Ning.,...&Wang, Jing.(2021).Structural characteristics and immune-enhancing activity of an extracellular polysaccharide produced by marine Halomonas sp. 2E1.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,183,1660-1668.
MLA	Wang, QingChi,et al."Structural characteristics and immune-enhancing activity of an extracellular polysaccharide produced by marine Halomonas sp. 2E1".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 183(2021):1660-1668.

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