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The features of an emerging whispovirus isolate from freshwater crayfish
Ke, Fei1,2,3; Gui, Lang4; Li, Tao1,2,3; Li, Fu-Hua5; Zhao, Xin1,2,3; Chen, Zhong-Yuan1,2,3; Lei, Cun-Ke1,2,3; Zhang, Qi-Ya1,2,3,4
2021-07-01
Source PublicationAQUACULTURE REPORTS
ISSN2352-5134
Volume20Pages:17
Corresponding AuthorZhang, Qi-Ya(zhangqy@ihb.ac.cn)
AbstractA highly lethal freshwater crayfish Cambarus clarkii whispovirus (WSSV-Cc) was isolated from a naturally infected host. A large number of viral particles with typical nimavirus characters were observed in host cells. Genomic architecture and genetic evolution of this extremely virulent pathogen were uncovered. WSSV-Cc has a double-stranded DNA genome containing 287,179 bp, encoding 180 putative ORFs. Congruence analysis was performed between WSSV-Cc and known nimavirus genomes. The genome of WSSV-Cc is smaller than that found in isolates sampled before 2010, but is similar in size to that of recent isolates, which provided new evidence for the shrinkage of nimavirus genome size during evolution. Phylogenetic analysis with the complete genome also showed connections between genome size and evolutionary relationship. Twenty low-similarity genes and three variable regions were identified from the WSSV-Cc genome. In addition, two recombination events were detected in the WSSV-Cc genome, and analysis of the recombinant regions showed a close relationship among the three freshwater crayfish nimaviruses. These findings provide us the morphological, genomic, and genetic characters of an emerging whispovirus, which will broaden our understanding to nimaviruses and be useful for diagnosis of crayfish viral disease.
KeywordNimaviridae Genome architecture Variable region Recombination Freshwater crayfish Cambarus clarkii whispovirus
DOI10.1016/j.aqrep.2021.100728
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Plan of the Ministry of Science and Technology of China[2018YFD0900302] ; National Key R&D Plan of the Ministry of Science and Technology of China[2020YFD0900303] ; National Natural Science Foundation of China[31772890] ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project[XDA24030203] ; Chinese Academy of Sciences[KJZD-SW-L11] ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2019FBZ04]
WOS Research AreaFisheries
WOS SubjectFisheries
WOS IDWOS:000674482200009
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/175746
Collection实验海洋生物学重点实验室
Corresponding AuthorZhang, Qi-Ya
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Coll Modern Agr Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China
4.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Int Res Ctr Marine Biosci,Minist Educ,Minist Sci, Key Lab Explorat & Utilizat Aquat Genet Resources, Shanghai, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Ke, Fei,Gui, Lang,Li, Tao,et al. The features of an emerging whispovirus isolate from freshwater crayfish[J]. AQUACULTURE REPORTS,2021,20:17.
APA Ke, Fei.,Gui, Lang.,Li, Tao.,Li, Fu-Hua.,Zhao, Xin.,...&Zhang, Qi-Ya.(2021).The features of an emerging whispovirus isolate from freshwater crayfish.AQUACULTURE REPORTS,20,17.
MLA Ke, Fei,et al."The features of an emerging whispovirus isolate from freshwater crayfish".AQUACULTURE REPORTS 20(2021):17.
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