Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp strain V134

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	Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp strain V134
	Zhang, Wei-wei; Sun, Li; Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
	2007
发表期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN	0099-2240
卷号	73 期号:9 页码:2825-2831
摘要	V134, a marine isolate of the Vibrio genus, was found to produce a new beta-agarase of the GH16 family. The relevant agarase gene agaV was cloned from V134 and conditionally expressed in Escherichia coli. Enzyme activity analysis revealed that the optimum temperature and pH for the purified recombinant agarase were around 40 degrees C and 7.0. AgaV was demonstrated to be useful in two aspects: first, as an agarolytic enzyme, the purified recombinant AgaV could be employed in the recovery of DNA from agarose gels; second, as a secretion protein, AgaV was explored at the genetic level and used as a reporter in the construction of a secretion signal trap which proved to be a simple and efficient molecular tool for the selection of genes encoding secretion proteins from both gram-positive and gram-negative bacteria.
关键词	Marine Bacterium Lactococcus-lactis Extracellular-agarase Glycosyl Hydrolases Secretion Signals Genomic Dna Purification Expression Classification Sequences
学科领域	Biotechnology & Applied Microbiology ; Microbiology
URL	查看原文
收录类别	sci
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1735
专题	实验海洋生物学重点实验室
通讯作者	Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wei-wei,Sun, Li,Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp strain V134[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2007,73(9):2825-2831.
APA	Zhang, Wei-wei,Sun, Li,&Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2007).Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp strain V134.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,73(9),2825-2831.
MLA	Zhang, Wei-wei,et al."Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp strain V134".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 73.9(2007):2825-2831.

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