Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong | |
Zhang, Rui ; Liu, Baozhong; Lau, Stanley C. K.; Ki, Jang-Seu; Qian, Pei-Yuan; Qian, PY, Hong Kong Univ Sci & Technol, Dept Biol & Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China
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| 2007-09-01 | |
| Source Publication | FEMS MICROBIOLOGY ECOLOGY
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| ISSN | 0168-6496 |
| Volume | 61Issue:3Pages:496-508 |
| Subtype | Article |
| Abstract | Diversity of particle-attached and free-living marine bacteria in Victoria Harbor, Hong Kong, and its adjacent coastal and estuarial environments was investigated using DNA fingerprinting and clone library analysis. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes showed that bacterial communities in three stations of Victoria Harbor were similar, but differed from those in adjacent coastal and estuarine stations. Particle-attached and free-living bacterial community composition differed in the Victoria Harbor area. DNA sequencing of 28 bands from DGGE gel showed Alphaproteobacteria was the most abundant group, followed by the Bacteroidetes, and other Proteobacteria. Bacterial species richness (number of DGGE bands) differed among stations and populations (particle-attached and free-living; bottom and surface). BIOENV analysis indicated that the concentrations of suspended solids were the major contributing parameter for the spatial variation of total bacterial community structure. Samples from representative stations were selected for clone library (548 clones) construction and their phylogenetic distributions were similar to those of sequences from DGGE. Approximately 80% of clones were affiliated to Proteobacteria, Bacteroidetes and Cyanobacteria. The possible influences of dynamic pollution and hydrological conditions in the Victoria Harbor area on the particle-attached and free-living bacterial community structures were discussed.; Diversity of particle-attached and free-living marine bacteria in Victoria Harbor, Hong Kong, and its adjacent coastal and estuarial environments was investigated using DNA fingerprinting and clone library analysis. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes showed that bacterial communities in three stations of Victoria Harbor were similar, but differed from those in adjacent coastal and estuarine stations. Particle-attached and free-living bacterial community composition differed in the Victoria Harbor area. DNA sequencing of 28 bands from DGGE gel showed Alphaproteobacteria was the most abundant group, followed by the Bacteroidetes, and other Proteobacteria. Bacterial species richness (number of DGGE bands) differed among stations and populations (particle-attached and free-living; bottom and surface). BIOENV analysis indicated that the concentrations of suspended solids were the major contributing parameter for the spatial variation of total bacterial community structure. Samples from representative stations were selected for clone library (548 clones) construction and their phylogenetic distributions were similar to those of sequences from DGGE. Approximately 80% of clones were affiliated to Proteobacteria, Bacteroidetes and Cyanobacteria. The possible influences of dynamic pollution and hydrological conditions in the Victoria Harbor area on the particle-attached and free-living bacterial community structures were discussed. |
| Keyword | Particle-attached Free-living Contrasting Marine Environment |
| Subject Area | Microbiology |
| DOI | 10.1111/j.1574-6941.2007.00353.x |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000248961900010 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/1725 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Qian, PY, Hong Kong Univ Sci & Technol, Dept Biol & Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China |
| Affiliation | 1.Hong Kong Univ Sci & Technol, Dept Biol & Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Rui,Liu, Baozhong,Lau, Stanley C. K.,et al. Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong[J]. FEMS MICROBIOLOGY ECOLOGY,2007,61(3):496-508. |
| APA | Zhang, Rui,Liu, Baozhong,Lau, Stanley C. K.,Ki, Jang-Seu,Qian, Pei-Yuan,&Qian, PY, Hong Kong Univ Sci & Technol, Dept Biol & Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China.(2007).Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong.FEMS MICROBIOLOGY ECOLOGY,61(3),496-508. |
| MLA | Zhang, Rui,et al."Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong".FEMS MICROBIOLOGY ECOLOGY 61.3(2007):496-508. |
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