Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain | |
Zhang, Min1,2; Sun, Kun1,2; Sun, Li1; Sun, L, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China | |
2008-07-01 | |
发表期刊 | MICROBIOLOGY-SGM |
ISSN | 1350-0872 |
卷号 | 154期号:Part 7页码:2060-2069 |
文章类型 | Article |
摘要 | Edwardsiella tarda is a bacterial pathogen that can infect both humans and animals. TX1, an Ed. tarda strain isolated from diseased fish, was found to produce autoinducer 2 (Al-2)-like activity that was growth phase dependent and modulated by growth conditions. The gene coding for the Al-2 synthase was cloned from TX1 and designated luxS(Et). LuxS(Et) was able to complement the Al-2 mutant phenotype of Escherichia coli strain DH5 alpha. Expression Of luxS(Et) correlated with Al-2 activity and was increased by glucose and decreased by elevated temperature. The effect of glucose was shown to be mediated through the cAMP-CRP complex, which repressed luxS(Et) expression. Overexpression of luxS(Et) enhanced Al-2 activity in TX1, whereas disruption of luxS(Et) expression by antisense RNA interference (i) reduced the level of Al-2 activity, (ii) impaired bacterial growth under various conditions, (iii) weakened the expression of genes associated with the type III secretion system and biofilm formation, and (iv) attenuated bacterial virulence. Addition of exogenous Al-2 was able to complement the deficiencies in the expression of TTSS genes and biofilm production but failed to rescue the growth defects. Our results (i) demonstrated that the Al-2 activity in TX1 is controlled at least in part at the level of luxS(Et) expression, which in turn is regulated by growth conditions, and that the temporal expression of luxS(Et) is essential for optimal bacterial infection and survival; and (ii) suggested the existence in Ed. tarda of a LuxS/Al-2-mediated signal transduction pathway that regulates the production of virulence-associated elements.; Edwardsiella tarda is a bacterial pathogen that can infect both humans and animals. TX1, an Ed. tarda strain isolated from diseased fish, was found to produce autoinducer 2 (Al-2)-like activity that was growth phase dependent and modulated by growth conditions. The gene coding for the Al-2 synthase was cloned from TX1 and designated luxS(Et). LuxS(Et) was able to complement the Al-2 mutant phenotype of Escherichia coli strain DH5 alpha. Expression Of luxS(Et) correlated with Al-2 activity and was increased by glucose and decreased by elevated temperature. The effect of glucose was shown to be mediated through the cAMP-CRP complex, which repressed luxS(Et) expression. Overexpression of luxS(Et) enhanced Al-2 activity in TX1, whereas disruption of luxS(Et) expression by antisense RNA interference (i) reduced the level of Al-2 activity, (ii) impaired bacterial growth under various conditions, (iii) weakened the expression of genes associated with the type III secretion system and biofilm formation, and (iv) attenuated bacterial virulence. Addition of exogenous Al-2 was able to complement the deficiencies in the expression of TTSS genes and biofilm production but failed to rescue the growth defects. Our results (i) demonstrated that the Al-2 activity in TX1 is controlled at least in part at the level of luxS(Et) expression, which in turn is regulated by growth conditions, and that the temporal expression of luxS(Et) is essential for optimal bacterial infection and survival; and (ii) suggested the existence in Ed. tarda of a LuxS/Al-2-mediated signal transduction pathway that regulates the production of virulence-associated elements. |
关键词 | Controls Biofilm Formation Quorum-sensing System Escherichia-coli Salmonella-typhimurium Vibrio-harveyi Streptococcus-pyogenes Gene-expression Iii Secretion Virulence Metabolism |
学科领域 | Microbiology |
DOI | 10.1099/mic.0.2008/017343-0 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000257893800022 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/1723 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Sun, L, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Zhang, Min,Sun, Kun,Sun, Li,et al. Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain[J]. MICROBIOLOGY-SGM,2008,154(Part 7):2060-2069. |
APA | Zhang, Min,Sun, Kun,Sun, Li,&Sun, L, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.(2008).Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain.MICROBIOLOGY-SGM,154(Part 7),2060-2069. |
MLA | Zhang, Min,et al."Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain".MICROBIOLOGY-SGM 154.Part 7(2008):2060-2069. |
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