Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain

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	Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain
	Zhang, Min; Sun, Kun; Sun, Li; Sun, L, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
	2008
发表期刊	MICROBIOLOGY-SGM
ISSN	1350-0872
卷号	154 期号:Part 7 页码:2060-2069
摘要	Edwardsiella tarda is a bacterial pathogen that can infect both humans and animals. TX1, an Ed. tarda strain isolated from diseased fish, was found to produce autoinducer 2 (Al-2)-like activity that was growth phase dependent and modulated by growth conditions. The gene coding for the Al-2 synthase was cloned from TX1 and designated luxS(Et). LuxS(Et) was able to complement the Al-2 mutant phenotype of Escherichia coli strain DH5 alpha. Expression Of luxS(Et) correlated with Al-2 activity and was increased by glucose and decreased by elevated temperature. The effect of glucose was shown to be mediated through the cAMP-CRP complex, which repressed luxS(Et) expression. Overexpression of luxS(Et) enhanced Al-2 activity in TX1, whereas disruption of luxS(Et) expression by antisense RNA interference (i) reduced the level of Al-2 activity, (ii) impaired bacterial growth under various conditions, (iii) weakened the expression of genes associated with the type III secretion system and biofilm formation, and (iv) attenuated bacterial virulence. Addition of exogenous Al-2 was able to complement the deficiencies in the expression of TTSS genes and biofilm production but failed to rescue the growth defects. Our results (i) demonstrated that the Al-2 activity in TX1 is controlled at least in part at the level of luxS(Et) expression, which in turn is regulated by growth conditions, and that the temporal expression of luxS(Et) is essential for optimal bacterial infection and survival; and (ii) suggested the existence in Ed. tarda of a LuxS/Al-2-mediated signal transduction pathway that regulates the production of virulence-associated elements.
关键词	Controls Biofilm Formation Quorum-sensing System Escherichia-coli Salmonella-typhimurium Vibrio-harveyi Streptococcus-pyogenes Gene-expression Iii Secretion Virulence Metabolism
学科领域	Microbiology
URL	查看原文
收录类别	sci
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1721
专题	实验海洋生物学重点实验室
通讯作者	Sun, L, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Min,Sun, Kun,Sun, Li,et al. Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain[J]. MICROBIOLOGY-SGM,2008,154(Part 7):2060-2069.
APA	Zhang, Min,Sun, Kun,Sun, Li,&Sun, L, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.(2008).Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain.MICROBIOLOGY-SGM,154(Part 7),2060-2069.
MLA	Zhang, Min,et al."Regulation of autoinducer 2 production and luxS expression in a pathogenic Edwardsiella tarda strain".MICROBIOLOGY-SGM 154.Part 7(2008):2060-2069.

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