IOCAS-IR  > 实验海洋生物学重点实验室
Attenuation of Edwardsiella tarda Virulence by Small Peptides That Interfere with LuxS/Autoinducer Type 2 Quorum Sensing
Zhang, Min; Jiao, Xu-dong; Hu, Yong-hua; Sun, Li; Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2009
发表期刊APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
卷号75期号:12页码:3882-3890
摘要Edwardsiella tarda is a gram-negative pathogen with a broad host range that includes humans, animals, and fish. Recent studies have shown that the LuxS/autoinducer type 2 (AI-2) quorum sensing system is involved in the virulence of E. tarda. In the present study, it was found that the E. tarda LuxS mutants bearing deletions of the catalytic site (C site) and the tyrosine kinase phosphorylation site, respectively, are functionally inactive and that these dysfunctional mutants can interfere with the activity of the wild-type LuxS. Two small peptides, 5411 and 5906, which share sequence identities with the C site of LuxS, were identified. 5411 and 5906 proved to be inhibitors of AI-2 activity and could vitiate the infectivity of the pathogenic E. tarda strain TX1. The inhibitory effect of 5411 and 5906 on AI-2 activity is exerted on LuxS, with which these peptides specifically interact. The expression of 5411 and 5906 in TX1 has multiple effects (altering biofilm production and the expression of certain virulence-associated genes), which are similar to those caused by interruption of luxS expression. Further study found that it is very likely that 5411 and 5906 can be released from the strains expressing them and, should TX1 be in the vicinity, captured by TX1. Based on this observation, a constitutive 5411 producer (Pseudomonas sp. strain FP3/pT5411) was constructed in the form of a fish commensal isolate that expresses 5411 from a plasmid source. The presence of FP3/pT5411 in fish attenuates the virulence of TX1. Finally, it was demonstrated that fish expressing 5411 directly from tissues exhibit enhanced resistance against TX1 infection.
关键词S-ribosylhomocysteinase Luxs Controls Biofilm Formation Aeruginosa Lung Infection Bacillus-subtilis Luxs Gram-negative Bacteria Pseudomonas-aeruginosa Escherichia-coli Vibrio-harveyi Salmonella-typhimurium Crystal-structure
学科领域Biotechnology & Applied Microbiology ; Microbiology
URL查看原文
收录类别sci
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/1719
专题实验海洋生物学重点实验室
通讯作者Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Min,Jiao, Xu-dong,Hu, Yong-hua,et al. Attenuation of Edwardsiella tarda Virulence by Small Peptides That Interfere with LuxS/Autoinducer Type 2 Quorum Sensing[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2009,75(12):3882-3890.
APA Zhang, Min,Jiao, Xu-dong,Hu, Yong-hua,Sun, Li,&Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2009).Attenuation of Edwardsiella tarda Virulence by Small Peptides That Interfere with LuxS/Autoinducer Type 2 Quorum Sensing.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,75(12),3882-3890.
MLA Zhang, Min,et al."Attenuation of Edwardsiella tarda Virulence by Small Peptides That Interfere with LuxS/Autoinducer Type 2 Quorum Sensing".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75.12(2009):3882-3890.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Zhang-2009-Attenuati(248KB) 限制开放--浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Min]的文章
[Jiao, Xu-dong]的文章
[Hu, Yong-hua]的文章
百度学术
百度学术中相似的文章
[Zhang, Min]的文章
[Jiao, Xu-dong]的文章
[Hu, Yong-hua]的文章
必应学术
必应学术中相似的文章
[Zhang, Min]的文章
[Jiao, Xu-dong]的文章
[Hu, Yong-hua]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Zhang-2009-Attenuation of Edwar1.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。