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Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification
Wang, Xiudan1; Li, Changmei1; Jia, Zhihao5; Xu, Tongxiao1; Wang, Yilin1; Sun, Mingzhu1; Han, Shuhui1; Wang, Xia1; Qiu, Limei2,3,4
2021-07-01
Source PublicationECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN0147-6513
Volume217Pages:10
Corresponding AuthorWang, Xia(wxbiology@qust.edu.cn) ; Qiu, Limei(qiulimei@qdio.ac.cn)
AbstractOcean acidification (OA) has posed formidable threats to marine calcifiers. In response to elevated CO2 levels, marine calcifiers have developed multiple strategies to survive, such as taking advantage of apoptosis, but its regulation mechanism remains largely unknown. Here, we used the Pacific oyster Crassostrea gigas as model to understand the apoptotic responses and regulation mechanism at short- (7 d) to long-term (56 d) CO2 exposure (pH = 7.50). The apoptosis of hemocytes was significantly induced after short-term treatment (7-21 d) but was suppressed under long-term CO2 exposure (42-56 d). Similarly, caspase-3 and caspase-9 were also increased post short-term exposure and fell back to normal levels after long-term exposure. These data together indicated diverse regulation mechanisms of apoptosis through different exposure periods. Through analysis of the B-cell lymphoma 2 (Bcl-2) family mitochondrial apoptosis regulators, we showed that only CgBcl-XL's expression kept at high levels after 42- and 56-day CO2 exposure. CgBcl-XL shared sequence, and structural similarity with its mammalian counterpart, and knockdown of CgBcl-XL in hemocytes via RNA interference promoted apoptosis. The protein level of CgBcl-XL was significantly increased after long-term CO2 exposure (28-56 d), and its distribution in hemocytes became more concentrated and dense. Therefore, CgBcl-XL serves as an essential antiapoptotic protein for tipping the balance of cell apoptosis, which may play a key role in survival under longterm CO2 exposure. These results reveal a potential adaptation strategy of oysters towards OA and the variable environment changes through the modulation of apoptosis.
KeywordOcean acidification Crassostrea gigas Apoptosis CgBcl-XL
DOI10.1016/j.ecoenv.2021.112235
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2019YFD0901705] ; Ministry of Science and Technology of the People's Republic of China
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000649634600007
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/170984
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Xia; Qiu, Limei
Affiliation1.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
5.Soochow Univ, Cambridge Suda Genom Resource Ctr, Suzhou 215123, Peoples R China
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Xiudan,Li, Changmei,Jia, Zhihao,et al. Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2021,217:10.
APA Wang, Xiudan.,Li, Changmei.,Jia, Zhihao.,Xu, Tongxiao.,Wang, Yilin.,...&Qiu, Limei.(2021).Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,217,10.
MLA Wang, Xiudan,et al."Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 217(2021):10.
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