Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification | |
Wang, Xiudan1 ; Li, Changmei1; Jia, Zhihao5; Xu, Tongxiao1; Wang, Yilin1; Sun, Mingzhu1; Han, Shuhui1; Wang, Xia1; Qiu, Limei2,3,4
| |
| 2021-07-01 | |
| Source Publication | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| ISSN | 0147-6513 |
| Volume | 217Pages:10 |
| Corresponding Author | Wang, Xia(wxbiology@qust.edu.cn) ; Qiu, Limei(qiulimei@qdio.ac.cn) |
| Abstract | Ocean acidification (OA) has posed formidable threats to marine calcifiers. In response to elevated CO2 levels, marine calcifiers have developed multiple strategies to survive, such as taking advantage of apoptosis, but its regulation mechanism remains largely unknown. Here, we used the Pacific oyster Crassostrea gigas as model to understand the apoptotic responses and regulation mechanism at short- (7 d) to long-term (56 d) CO2 exposure (pH = 7.50). The apoptosis of hemocytes was significantly induced after short-term treatment (7-21 d) but was suppressed under long-term CO2 exposure (42-56 d). Similarly, caspase-3 and caspase-9 were also increased post short-term exposure and fell back to normal levels after long-term exposure. These data together indicated diverse regulation mechanisms of apoptosis through different exposure periods. Through analysis of the B-cell lymphoma 2 (Bcl-2) family mitochondrial apoptosis regulators, we showed that only CgBcl-XL's expression kept at high levels after 42- and 56-day CO2 exposure. CgBcl-XL shared sequence, and structural similarity with its mammalian counterpart, and knockdown of CgBcl-XL in hemocytes via RNA interference promoted apoptosis. The protein level of CgBcl-XL was significantly increased after long-term CO2 exposure (28-56 d), and its distribution in hemocytes became more concentrated and dense. Therefore, CgBcl-XL serves as an essential antiapoptotic protein for tipping the balance of cell apoptosis, which may play a key role in survival under longterm CO2 exposure. These results reveal a potential adaptation strategy of oysters towards OA and the variable environment changes through the modulation of apoptosis. |
| Keyword | Ocean acidification Crassostrea gigas Apoptosis CgBcl-XL |
| DOI | 10.1016/j.ecoenv.2021.112235 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key Research and Development Program of China[2019YFD0901705] ; Ministry of Science and Technology of the People's Republic of China |
| WOS Research Area | Environmental Sciences & Ecology ; Toxicology |
| WOS Subject | Environmental Sciences ; Toxicology |
| WOS ID | WOS:000649634600007 |
| Publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/170984 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Wang, Xia; Qiu, Limei |
| Affiliation | 1.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 5.Soochow Univ, Cambridge Suda Genom Resource Ctr, Suzhou 215123, Peoples R China |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Wang, Xiudan,Li, Changmei,Jia, Zhihao,et al. Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2021,217:10. |
| APA | Wang, Xiudan.,Li, Changmei.,Jia, Zhihao.,Xu, Tongxiao.,Wang, Yilin.,...&Qiu, Limei.(2021).Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,217,10. |
| MLA | Wang, Xiudan,et al."Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 217(2021):10. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 1-s2.0-S014765132100（4256KB） | 期刊论文 | 出版稿 | 延迟开放 | CC BY-NC-SA | View 2023-7-1后可获取 | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment