Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification

doi:10.1016/j.ecoenv.2021.112235

IOCAS-IR > 实验海洋生物学重点实验室

	Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification
	Wang, Xiudan1 ; Li, Changmei 1; Jia, Zhihao 5; Xu, Tongxiao 1; Wang, Yilin 1; Sun, Mingzhu 1; Han, Shuhui 1; Wang, Xia1 ; Qiu, Limei 2,3,4
	2021-07-01
发表期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
卷号	217 页码:10
通讯作者	Wang, Xia(wxbiology@qust.edu.cn) ; Qiu, Limei(qiulimei@qdio.ac.cn)
摘要	Ocean acidification (OA) has posed formidable threats to marine calcifiers. In response to elevated CO2 levels, marine calcifiers have developed multiple strategies to survive, such as taking advantage of apoptosis, but its regulation mechanism remains largely unknown. Here, we used the Pacific oyster Crassostrea gigas as model to understand the apoptotic responses and regulation mechanism at short- (7 d) to long-term (56 d) CO2 exposure (pH = 7.50). The apoptosis of hemocytes was significantly induced after short-term treatment (7-21 d) but was suppressed under long-term CO2 exposure (42-56 d). Similarly, caspase-3 and caspase-9 were also increased post short-term exposure and fell back to normal levels after long-term exposure. These data together indicated diverse regulation mechanisms of apoptosis through different exposure periods. Through analysis of the B-cell lymphoma 2 (Bcl-2) family mitochondrial apoptosis regulators, we showed that only CgBcl-XL's expression kept at high levels after 42- and 56-day CO2 exposure. CgBcl-XL shared sequence, and structural similarity with its mammalian counterpart, and knockdown of CgBcl-XL in hemocytes via RNA interference promoted apoptosis. The protein level of CgBcl-XL was significantly increased after long-term CO2 exposure (28-56 d), and its distribution in hemocytes became more concentrated and dense. Therefore, CgBcl-XL serves as an essential antiapoptotic protein for tipping the balance of cell apoptosis, which may play a key role in survival under longterm CO2 exposure. These results reveal a potential adaptation strategy of oysters towards OA and the variable environment changes through the modulation of apoptosis.
关键词	Ocean acidification Crassostrea gigas Apoptosis CgBcl-XL
DOI	10.1016/j.ecoenv.2021.112235
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2019YFD0901705] ; Ministry of Science and Technology of the People's Republic of China
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
WOS类目	Environmental Sciences ; Toxicology
WOS记录号	WOS:000649634600007
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170984
专题	实验海洋生物学重点实验室
通讯作者	Wang, Xia; Qiu, Limei
作者单位	1.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 5.Soochow Univ, Cambridge Suda Genom Resource Ctr, Suzhou 215123, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Xiudan,Li, Changmei,Jia, Zhihao,et al. Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2021,217:10.
APA	Wang, Xiudan.,Li, Changmei.,Jia, Zhihao.,Xu, Tongxiao.,Wang, Yilin.,...&Qiu, Limei.(2021).Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,217,10.
MLA	Wang, Xiudan,et al."Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 217(2021):10.

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