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A Lymphoid Organ Specific Anti-Lipopolysaccharide Factor from Litopenaeus vannamei Exhibits Strong Antimicrobial Activities
Sun, Mingzhe1; Li, Shihao1,2,3; Lv, Xinjia1; Xiang, Jianhai1,2,3; Lu, Yuanan4; Li, Fuhua1,2,3,5
2021-05-01
Source PublicationMARINE DRUGS
Volume19Issue:5Pages:15
Corresponding AuthorLi, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
AbstractDifferent shrimp species are known to possess apparent distinct resistance to different pathogens in aquaculture. However, the molecular mechanism underlying this finding still remains unknown. One kind of important antimicrobial peptides, anti-lipopolysaccharide factors (ALF), exhibit broad-spectrum antimicrobial activities. Here, we reported a newly identified ALF from the shrimp Litopenaeus vannamei and compared the immune function with its counterpart in the shrimp Fenneropenaeus chinensis. The ALF, designated as LvALF8, was specifically expressed in the lymphoid organ of L. vannamei. The expression level of LvALF8 was apparently changed after white spot syndrome virus (WSSV) or Vibrio parahaemolyticus challenges. The synthetic LBD peptide of LvALF8 (LvALF8-LBD) showed strong antibacterial activities against most tested Gram-negative and Gram-positive bacteria. LvALF8-LBD could also inhibit the in vivo propagation of WSSV similar as FcALF8-LBD, the LBD of LvALF8 counterpart in F. chinensis. However, LvALF8-LBD and FcALF8-LBD exhibited apparently different antibacterial activity against V. parahaemolyticus, the main pathogen causing acute hepatopancreatic necrosis disease (AHPND) of affected shrimp. A structural analysis showed that the positive net charge and amphipathicity characteristics of LvALF8-LBD peptide were speculated as two important components for its enhanced antimicrobial activity compared to those of FcALF8-LBD. These new findings may not only provide some evidence to explain the distinct disease resistance among different shrimp species, but also lay out new research ground for the testing and development of LBD-originated antimicrobial peptides to control of shrimp diseases.
Keywordanti-lipopolysaccharide factor LPS-binding domain lymphoid organ white spot syndrome virus Vibro parahaemolyticus
DOI10.3390/md19050250
Indexed BySCI
Language英语
Funding ProjectKey Program of National Natural Science Foundation of China[31830100] ; General Program of National Natural Science Foundation of China[31772880] ; General Program of National Natural Science Foundation of China[41776158] ; China Agriculture Research system-48 (CARS-48) ; Distinguished Young Scientists Research Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KLEMB-DYS01]
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal ; Pharmacology & Pharmacy
WOS IDWOS:000654297500001
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/170947
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Shihao; Li, Fuhua
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Univ Hawaii Manoa, Dept Publ Hlth Sci, Environm Hlth Lab, Honolulu, HI 96822 USA
5.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Sun, Mingzhe,Li, Shihao,Lv, Xinjia,et al. A Lymphoid Organ Specific Anti-Lipopolysaccharide Factor from Litopenaeus vannamei Exhibits Strong Antimicrobial Activities[J]. MARINE DRUGS,2021,19(5):15.
APA Sun, Mingzhe,Li, Shihao,Lv, Xinjia,Xiang, Jianhai,Lu, Yuanan,&Li, Fuhua.(2021).A Lymphoid Organ Specific Anti-Lipopolysaccharide Factor from Litopenaeus vannamei Exhibits Strong Antimicrobial Activities.MARINE DRUGS,19(5),15.
MLA Sun, Mingzhe,et al."A Lymphoid Organ Specific Anti-Lipopolysaccharide Factor from Litopenaeus vannamei Exhibits Strong Antimicrobial Activities".MARINE DRUGS 19.5(2021):15.
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