Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Comparation of oxylipin profiles as well as their substrates and synthetic enzymes transcriptional expression between marine fish Larimichthys crocea and freshwater fish Oncorhynchus mykiss | |
| Gao, Shengnan1,2; Cui, Kun1,2; Li, Yongnan1,2,3; Pang, Yuning1,2; Fang, Wei1,2; Mai, Kangsen1,2; Ai, Qinghui1,2 | |
| 2021-06-30 | |
| Source Publication | AQUACULTURE
 |
| ISSN | 0044-8486 |
| Volume | 539Pages:12 |
| Corresponding Author | Ai, Qinghui(qhai@ouc.edu.cn) |
| Abstract | Oxylipins formed from polyunsaturated fatty acids (PUFAs) play an important role in normal physiology and function. Therefore, oxylipin profiles have been revealed in many species such as human, rats and even nonmammals. However, it is rarely studied in fish, a species known to be an excellent source of LC-PUFAs in human diets. In the present study, Larimichthys crocea and Oncorhynchus mykiss were selected as the marine and the freshwater fish model, respectively, used to quantify the oxylipin profiles by liquid chromatography tandem mass spectrometry and further synthesis related enzymes expression of the oxylipins were determined by RNAseq. A total of 57 oxylipins were quantified and arachidonic acid oxylipins dominated which occupied 42.1%, and oxylipins derived from DHA and EPA ranked the second (17.6%) and third (15.8%). Thirty differential oxylipins were screened and analyzed to compare the differences between L. crocea and O. mykiss. It could be concluded that L. crocea may be better at utilizing substrate fatty acids to synthesize oxylipins than O. mykiss, and the different expression of the synthetic related enzymes probably accounted for the differences. 12-HETE in O. mykiss and PGJ2 in L. crocea were the most representative oxylipins to distinguish the two fishes. In conclusion, our study was the first study comparing the oxylipin profiles between marine fish and freshwater fish, and the representative differential oxylipins may serve as markers, providing a new insight of identifying and distinguishing marine and freshwater fish species. |
| Keyword | Oxylipin profiles Cyclooxygenase Lipoxygenase Cytochrome Marine fish Freshwater fish |
| DOI | 10.1016/j.aquaculture.2021.736641 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Key Program of National Natural Science Foundation of China[31830103] ; National Science Fund for Distinguished Young Scholars of China[31525024] |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000647595100009 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/170929 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Ai, Qinghui |
| Affiliation | 1.Ocean Univ China, Minist Educ, Key Lab Mariculture, Key Lab Aquaculture Nutr & Feed,Minist Agr, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China |
| Recommended Citation GB/T 7714 | Gao, Shengnan,Cui, Kun,Li, Yongnan,et al. Comparation of oxylipin profiles as well as their substrates and synthetic enzymes transcriptional expression between marine fish Larimichthys crocea and freshwater fish Oncorhynchus mykiss[J]. AQUACULTURE,2021,539:12. |
| APA | Gao, Shengnan.,Cui, Kun.,Li, Yongnan.,Pang, Yuning.,Fang, Wei.,...&Ai, Qinghui.(2021).Comparation of oxylipin profiles as well as their substrates and synthetic enzymes transcriptional expression between marine fish Larimichthys crocea and freshwater fish Oncorhynchus mykiss.AQUACULTURE,539,12. |
| MLA | Gao, Shengnan,et al."Comparation of oxylipin profiles as well as their substrates and synthetic enzymes transcriptional expression between marine fish Larimichthys crocea and freshwater fish Oncorhynchus mykiss".AQUACULTURE 539(2021):12. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 1-s2.0-S004484862100（4297KB） | 期刊论文 | 出版稿 | 延迟开放 | CC BY-NC-SA | View 2023-7-1后可获取 | |
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