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Comparation of oxylipin profiles as well as their substrates and synthetic enzymes transcriptional expression between marine fish Larimichthys crocea and freshwater fish Oncorhynchus mykiss
Gao, Shengnan1,2; Cui, Kun1,2; Li, Yongnan1,2,3; Pang, Yuning1,2; Fang, Wei1,2; Mai, Kangsen1,2; Ai, Qinghui1,2
2021-06-30
Source PublicationAQUACULTURE
ISSN0044-8486
Volume539Pages:12
Corresponding AuthorAi, Qinghui(qhai@ouc.edu.cn)
AbstractOxylipins formed from polyunsaturated fatty acids (PUFAs) play an important role in normal physiology and function. Therefore, oxylipin profiles have been revealed in many species such as human, rats and even nonmammals. However, it is rarely studied in fish, a species known to be an excellent source of LC-PUFAs in human diets. In the present study, Larimichthys crocea and Oncorhynchus mykiss were selected as the marine and the freshwater fish model, respectively, used to quantify the oxylipin profiles by liquid chromatography tandem mass spectrometry and further synthesis related enzymes expression of the oxylipins were determined by RNAseq. A total of 57 oxylipins were quantified and arachidonic acid oxylipins dominated which occupied 42.1%, and oxylipins derived from DHA and EPA ranked the second (17.6%) and third (15.8%). Thirty differential oxylipins were screened and analyzed to compare the differences between L. crocea and O. mykiss. It could be concluded that L. crocea may be better at utilizing substrate fatty acids to synthesize oxylipins than O. mykiss, and the different expression of the synthetic related enzymes probably accounted for the differences. 12-HETE in O. mykiss and PGJ2 in L. crocea were the most representative oxylipins to distinguish the two fishes. In conclusion, our study was the first study comparing the oxylipin profiles between marine fish and freshwater fish, and the representative differential oxylipins may serve as markers, providing a new insight of identifying and distinguishing marine and freshwater fish species.
KeywordOxylipin profiles Cyclooxygenase Lipoxygenase Cytochrome Marine fish Freshwater fish
DOI10.1016/j.aquaculture.2021.736641
Indexed BySCI
Language英语
Funding ProjectKey Program of National Natural Science Foundation of China[31830103] ; National Science Fund for Distinguished Young Scholars of China[31525024]
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000647595100009
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/170929
Collection实验海洋生物学重点实验室
Corresponding AuthorAi, Qinghui
Affiliation1.Ocean Univ China, Minist Educ, Key Lab Mariculture, Key Lab Aquaculture Nutr & Feed,Minist Agr, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Gao, Shengnan,Cui, Kun,Li, Yongnan,et al. Comparation of oxylipin profiles as well as their substrates and synthetic enzymes transcriptional expression between marine fish Larimichthys crocea and freshwater fish Oncorhynchus mykiss[J]. AQUACULTURE,2021,539:12.
APA Gao, Shengnan.,Cui, Kun.,Li, Yongnan.,Pang, Yuning.,Fang, Wei.,...&Ai, Qinghui.(2021).Comparation of oxylipin profiles as well as their substrates and synthetic enzymes transcriptional expression between marine fish Larimichthys crocea and freshwater fish Oncorhynchus mykiss.AQUACULTURE,539,12.
MLA Gao, Shengnan,et al."Comparation of oxylipin profiles as well as their substrates and synthetic enzymes transcriptional expression between marine fish Larimichthys crocea and freshwater fish Oncorhynchus mykiss".AQUACULTURE 539(2021):12.
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