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Lipid metabolism changes in clam Meretrix petechialis in response to Vibrio infection and the identification of Vibrio-resistance markers
Su, Rina1,3; Fang, Jun4; Wang, Hongxia1,2; Liu, Baozhong1,2
2021-06-30
Source PublicationAQUACULTURE
ISSN0044-8486
Volume539Pages:11
Corresponding AuthorWang, Hongxia(hxwang@qdio.ac.cn)
AbstractMass summer mortality events in the clam Meretrix petechialis resulting from Vibrio infection have occurred frequently in recent years, and seriously threaten the clam aquaculture industry. Metabolic processes are known to support host defense and survival in stressful conditions by regulating the allocation of metabolic resources. However, changes in lipid metabolism of clams in response to Vibrio infection are largely undescribed. In the present research, we characterized changes in key gene expression and enzyme activities associated with triglyceride-related metabolism, fatty acid de novo synthesis and fatty acid ?-oxidation in M. petechialis during Vibrio infection. By mining dynamic transcriptome data before the increase in mortality, we found Vibrio infection caused significant changes in the metabolic regulations of carbohydrates, lipids, and amino acids. Our data show that levels of triglycerides (TG) decreased and free fatty acids (FFA) increased in the infected clams. Correspondingly, the synthesis activities of TG were significantly reduced relative to TG lipolysis activities after Vibrio infection. Additionally, FFA de novo synthesis was strengthened, especially before massive mortality occurred, but key genes of the fatty acid ?-oxidation pathway showed no significant changes after Vibrio infection. We detected the expression of key genes related to lipid metabolism in resistant and susceptible clams under uninfected and infected conditions and found that differences in the basal expression of seven key genes (ATGL, MDY, SREBP, FAS, CPT-1, ACS, and ECH) could be used as potential biomarkers to predict different Vibrio resistance in clams. Overall, our results provide a starting point for investigating the relationship between energy metabolism changes and the emergence of massive mortality induced by bacterial infection in clams.
KeywordMeretrix petechialis Vibiro parahaemolyticus Lipid metabolism Mortality Gene expression
DOI10.1016/j.aquaculture.2021.736611
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31772845] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030304] ; China Agriculture Research System[CARS49] ; National Key R & D Program of China[2019YFD0900700] ; National Science Foundation of Shandong Province, China[ZR2020MD089]
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000647595900009
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/170926
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Hongxia
Affiliation1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Zhejiang Mariculture Res Inst, Wenzhou 325005, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Su, Rina,Fang, Jun,Wang, Hongxia,et al. Lipid metabolism changes in clam Meretrix petechialis in response to Vibrio infection and the identification of Vibrio-resistance markers[J]. AQUACULTURE,2021,539:11.
APA Su, Rina,Fang, Jun,Wang, Hongxia,&Liu, Baozhong.(2021).Lipid metabolism changes in clam Meretrix petechialis in response to Vibrio infection and the identification of Vibrio-resistance markers.AQUACULTURE,539,11.
MLA Su, Rina,et al."Lipid metabolism changes in clam Meretrix petechialis in response to Vibrio infection and the identification of Vibrio-resistance markers".AQUACULTURE 539(2021):11.
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