IOCAS-IR  > 实验海洋生物学重点实验室
Lipid metabolism changes in clam Meretrix petechialis in response to Vibrio infection and the identification of Vibrio-resistance markers
Su, Rina1,3; Fang, Jun4; Wang, Hongxia1,2; Liu, Baozhong1,2
2021-06-30
发表期刊AQUACULTURE
ISSN0044-8486
卷号539页码:11
通讯作者Wang, Hongxia(hxwang@qdio.ac.cn)
摘要Mass summer mortality events in the clam Meretrix petechialis resulting from Vibrio infection have occurred frequently in recent years, and seriously threaten the clam aquaculture industry. Metabolic processes are known to support host defense and survival in stressful conditions by regulating the allocation of metabolic resources. However, changes in lipid metabolism of clams in response to Vibrio infection are largely undescribed. In the present research, we characterized changes in key gene expression and enzyme activities associated with triglyceride-related metabolism, fatty acid de novo synthesis and fatty acid ?-oxidation in M. petechialis during Vibrio infection. By mining dynamic transcriptome data before the increase in mortality, we found Vibrio infection caused significant changes in the metabolic regulations of carbohydrates, lipids, and amino acids. Our data show that levels of triglycerides (TG) decreased and free fatty acids (FFA) increased in the infected clams. Correspondingly, the synthesis activities of TG were significantly reduced relative to TG lipolysis activities after Vibrio infection. Additionally, FFA de novo synthesis was strengthened, especially before massive mortality occurred, but key genes of the fatty acid ?-oxidation pathway showed no significant changes after Vibrio infection. We detected the expression of key genes related to lipid metabolism in resistant and susceptible clams under uninfected and infected conditions and found that differences in the basal expression of seven key genes (ATGL, MDY, SREBP, FAS, CPT-1, ACS, and ECH) could be used as potential biomarkers to predict different Vibrio resistance in clams. Overall, our results provide a starting point for investigating the relationship between energy metabolism changes and the emergence of massive mortality induced by bacterial infection in clams.
关键词Meretrix petechialis Vibiro parahaemolyticus Lipid metabolism Mortality Gene expression
DOI10.1016/j.aquaculture.2021.736611
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[31772845] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030304] ; China Agriculture Research System[CARS49] ; National Key R & D Program of China[2019YFD0900700] ; National Science Foundation of Shandong Province, China[ZR2020MD089]
WOS研究方向Fisheries ; Marine & Freshwater Biology
WOS类目Fisheries ; Marine & Freshwater Biology
WOS记录号WOS:000647595900009
出版者ELSEVIER
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/170926
专题实验海洋生物学重点实验室
通讯作者Wang, Hongxia
作者单位1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Zhejiang Mariculture Res Inst, Wenzhou 325005, Peoples R China
第一作者单位中国科学院海洋研究所
通讯作者单位中国科学院海洋研究所
推荐引用方式
GB/T 7714
Su, Rina,Fang, Jun,Wang, Hongxia,et al. Lipid metabolism changes in clam Meretrix petechialis in response to Vibrio infection and the identification of Vibrio-resistance markers[J]. AQUACULTURE,2021,539:11.
APA Su, Rina,Fang, Jun,Wang, Hongxia,&Liu, Baozhong.(2021).Lipid metabolism changes in clam Meretrix petechialis in response to Vibrio infection and the identification of Vibrio-resistance markers.AQUACULTURE,539,11.
MLA Su, Rina,et al."Lipid metabolism changes in clam Meretrix petechialis in response to Vibrio infection and the identification of Vibrio-resistance markers".AQUACULTURE 539(2021):11.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
1-s2.0-S004484862100(3181KB)期刊论文出版稿限制开放CC BY-NC-SA浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Su, Rina]的文章
[Fang, Jun]的文章
[Wang, Hongxia]的文章
百度学术
百度学术中相似的文章
[Su, Rina]的文章
[Fang, Jun]的文章
[Wang, Hongxia]的文章
必应学术
必应学术中相似的文章
[Su, Rina]的文章
[Fang, Jun]的文章
[Wang, Hongxia]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 1-s2.0-S0044848621002738-main.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。