IOCAS-IR  > 海洋生态与环境科学重点实验室
A Toll-like receptor identified in Gigantidas platifrons and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria
Li, Mengna1,2,3,4; Chen, Hao1,2,3,5,6; Wang, Minxiao1,2,3,5,6; Zhong, Zhaoshan1,2,3,5,6; Wang, Hao1,2,3,5,6; Zhou, Li1,2,3,5,6; Zhang, Huan1,2,3,5,6; Li, Chaolun1,2,3,4,5,6
2021-04-30
发表期刊PEERJ
ISSN2167-8359
卷号9页码:21
通讯作者Li, Chaolun(lcl@qdio.ac.cn)
摘要Symbiosis with chemosynthetic bacteria is an important ecological strategy for the deep-sea megafaunas including mollusks, tubeworms and crustacean to obtain nutrients in hydrothermal vents and cold seeps. How the megafaunas recognize symbionts and establish the symbiosis has attracted much attention. Bathymodiolinae mussels are endemic species in both hydrothermal vents and cold seeps while the immune recognition mechanism underlying the symbiosis is not well understood due to the nonculturable symbionts. In previous study, a lipopolysaccharide (LPS) pull-down assay was conducted in Gigantidas platifrons to screen the pattern recognition receptors potentially involved in the recognition of symbiotic methane-oxidizing bacteria (MOB). Consequently, a total of 208 proteins including GpTLR13 were identified. Here the molecular structure, expression pattern and immune function of GpTLR13 were further analyzed. It was found that GpTLR13 could bind intensively with the lipid A structure of LPS through surface plasmon resonance analysis. The expression alternations of GpTLR13 transcripts during a 28-day of symbiont-depletion assay were investigated by real-time qPCR. As a result, a robust decrease of GpTLR13 transcripts was observed accompanying with the loss of symbionts, implying its participation in symbiosis. In addition, GpTLR13 transcripts were found expressed exclusively in the bacteriocytes of gills of G. platifrons by in situ hybridization. It was therefore speculated that GpTLR13 may be involved in the immune recognition of symbiotic methane-oxidizing bacteria by specifically recognizing the lipid A structure of LPS. However, the interaction between GpTLR13 and symbiotic MOB was failed to be addressed due to the nonculturable symbionts. Nevertheless, the present result has provided with a promising candidate as well as a new approach for the identification of symbiont-related genes in Bathymodiolinae mussels.
关键词Gigantidas platifrons Methane-oxidizing bacteria Symbiosis Immune recognition Toll like receptor Pattern recognition receptors
DOI10.7717/peerj.11282
收录类别SCI
语种英语
资助项目Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050303] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42020401] ; Key Research Program of Frontier Sciences, CAS[ZDBS-LY-DQC032] ; National Key Research and Development Program of China[2018YFC0310800] ; National Natural Science Foundation of China[42030407] ; National Natural Science Foundation of China[42076091] ; National Natural Science Foundation of China[41906103] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, CAS[COMS2020Q02]
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000647021700004
出版者PEERJ INC
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/170917
专题海洋生态与环境科学重点实验室
深海极端环境与生命过程研究中心
通讯作者Li, Chaolun
作者单位1.Chinese Acad Sci, Ctr Deep Sea Res, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci CODR, Qingdao, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, KLMEES, Qingdao, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Qingdao Natl Lab Marine Sci & Technol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
6.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
第一作者单位中国科学院海洋研究所
通讯作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
推荐引用方式
GB/T 7714
Li, Mengna,Chen, Hao,Wang, Minxiao,et al. A Toll-like receptor identified in Gigantidas platifrons and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria[J]. PEERJ,2021,9:21.
APA Li, Mengna.,Chen, Hao.,Wang, Minxiao.,Zhong, Zhaoshan.,Wang, Hao.,...&Li, Chaolun.(2021).A Toll-like receptor identified in Gigantidas platifrons and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria.PEERJ,9,21.
MLA Li, Mengna,et al."A Toll-like receptor identified in Gigantidas platifrons and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria".PEERJ 9(2021):21.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
peerj-11282.pdf(8606KB)期刊论文出版稿限制开放CC BY-NC-SA浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Mengna]的文章
[Chen, Hao]的文章
[Wang, Minxiao]的文章
百度学术
百度学术中相似的文章
[Li, Mengna]的文章
[Chen, Hao]的文章
[Wang, Minxiao]的文章
必应学术
必应学术中相似的文章
[Li, Mengna]的文章
[Chen, Hao]的文章
[Wang, Minxiao]的文章
相关权益政策
暂无数据
收藏/分享
文件名: peerj-11282.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。