Profiling of Brevetoxin Metabolites Produced by Karenia brevis 165 Based on Liquid Chromatography-Mass Spectrometry

doi:10.3390/toxins13050354

	Profiling of Brevetoxin Metabolites Produced by Karenia brevis 165 Based on Liquid Chromatography-Mass Spectrometry
	Shen, Huihui 1,2,3; Song, Xiuxian1,2,3,4 ; Zhang, Yue 1,2; Zhang, Peipei 1,2,3; Li, Jing1,2 ; Song, Weijia 1,2,3; Yu, Zhiming1,2,3,4
	2021-05-01
发表期刊	TOXINS
卷号	13 期号:5 页码:16
通讯作者	Song, Xiuxian(songxx@qdio.ac.cn)
摘要	In this study, Karenia brevis 165 (K. brevis 165), a Chinese strain, was used to research brevetoxin (BTX) metabolites. The sample pretreatment method for the enrichment of BTX metabolites in an algal culture medium was improved here. The method for screening and identifying intracellular and extracellular BTX metabolites was established based on liquid chromatography-time-of-flight mass spectrometry (LC-ToF-MS) and liquid chromatography triple quadrupole tandem mass spectrometry (LC-QqQ-MS/MS). The results show that the recovery rates for BTX toxins enriched by a hydrophilic-lipophilic balance (HLB) extraction column were higher than those with a C18 extraction column. This method was used to analyze the profiles of extracellular and intracellular BTX metabolites at different growth stages of K. brevis 165. This is the first time a Chinese strain of K. brevis has been reported that can produce toxic BTX metabolites. Five and eight kinds of BTX toxin metabolites were detected in the cell and culture media of K. brevis 165, respectively. Brevenal, a toxic BTX metabolite antagonist, was found for the first time in the culture media. The toxic BTX metabolites and brevenal in the K. brevis 165 cell and culture media were found to be fully proven in terms of the necessity of establishing a method for screening and identifying toxic BTX metabolites. The results found by qualitatively and quantitatively analyzing BTX metabolites produced by K. brevis 165 at different growth stages show that the total toxic BTX metabolite contents in single cells ranged between 6.78 and 21.53 pg/cell, and the total toxin concentration in culture media ranged between 10.27 and 449.11 mu g/L. There were significant differences in the types and contents of toxic BTX metabolites with varying growth stages. Therefore, when harmful algal blooms occur, the accurate determination of BTX metabolite types and concentrations will be helpful to assess the ecological disaster risk in order to avoid hazards and provide appropriate disaster warnings.
关键词	Karenia brevis 165 profile brevetoxin metabolites liquid chromatography-mass spectrometry
DOI	10.3390/toxins13050354
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41976148] ; Taishan Scholars Climbing Program of Shandong Province of 2019 ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0504-2]
WOS研究方向	Food Science & Technology ; Toxicology
WOS类目	Food Science & Technology ; Toxicology
WOS记录号	WOS:000654605100001
出版者	MDPI
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170896
专题	海洋生态与环境科学重点实验室
通讯作者	Song, Xiuxian
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Shen, Huihui,Song, Xiuxian,Zhang, Yue,et al. Profiling of Brevetoxin Metabolites Produced by Karenia brevis 165 Based on Liquid Chromatography-Mass Spectrometry[J]. TOXINS,2021,13(5):16.
APA	Shen, Huihui.,Song, Xiuxian.,Zhang, Yue.,Zhang, Peipei.,Li, Jing.,...&Yu, Zhiming.(2021).Profiling of Brevetoxin Metabolites Produced by Karenia brevis 165 Based on Liquid Chromatography-Mass Spectrometry.TOXINS,13(5),16.
MLA	Shen, Huihui,et al."Profiling of Brevetoxin Metabolites Produced by Karenia brevis 165 Based on Liquid Chromatography-Mass Spectrometry".TOXINS 13.5(2021):16.

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