IOCAS-IR  > 实验海洋生物学重点实验室
Edwardsiella tarda outer membrane protein 1 is a virulence factor and a protective immunogen
Jin, Cheng-dong1,2,3; Li, Mo-fei1,2
2021-06-30
Source PublicationAQUACULTURE
ISSN0044-8486
Volume539Pages:9
Corresponding AuthorLi, Mo-fei(limofei@qdio.ac.cn)
AbstractThe bacterial outer membrane proteins are important components of bacterial cell. Edwardsiella tarda is a vital bacterial pathogen to a wide range of aquaculture animals. In this study, we examined the function of an outer membrane protein of E. tarda (named Omp1Et). Omp1Et was found to localize on the surface of E. tarda, and recombinant Omp1Et (rOmp1Et) was able to interact with flounder peripheral blood leukocytes. Compared to the wild type, the omp1Et knockout TX01?omp1 was significantly reduced in motility, biofilm formation, attachment and invasion into host cells, dissemination in host tissues, and inducing host mortality. Furthermore, TX01?omp1 was significantly impaired in the ability to survive under low pH and oxidizing conditions. When introduced into flounder as a subunit vaccine, rOmp1Et induced strong immune protection against E. tarda challenge. Taken together, these results indicate that Omp1Et is a surface-located virulence factor of E. tarda and rOmp1Et can induce effective immune-protection as a submit vaccine.
KeywordEdwardsiella tarda Outer membrane protein Virulence Vaccine
DOI10.1016/j.aquaculture.2021.736596
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31972831] ; National Key Research and Development Program of China[2018YFD0900501] ; Youth Innovation Promotion Association of Chinese Academy of Sciences[2017249] ; Huiquan Young Scholar Program of Institute of Oceanology, CAS ; Taishan Scholar Program of Shandong Province
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000644225600001
PublisherELSEVIER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/170869
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Mo-fei
Affiliation1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jin, Cheng-dong,Li, Mo-fei. Edwardsiella tarda outer membrane protein 1 is a virulence factor and a protective immunogen[J]. AQUACULTURE,2021,539:9.
APA Jin, Cheng-dong,&Li, Mo-fei.(2021).Edwardsiella tarda outer membrane protein 1 is a virulence factor and a protective immunogen.AQUACULTURE,539,9.
MLA Jin, Cheng-dong,et al."Edwardsiella tarda outer membrane protein 1 is a virulence factor and a protective immunogen".AQUACULTURE 539(2021):9.
Files in This Item:
File Name/Size DocType Version Access License
1-s2.0-S004484862100(3824KB)期刊论文出版稿延迟开放CC BY-NC-SAView 2023-7-1后可获取
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jin, Cheng-dong]'s Articles
[Li, Mo-fei]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jin, Cheng-dong]'s Articles
[Li, Mo-fei]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jin, Cheng-dong]'s Articles
[Li, Mo-fei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 1-s2.0-S0044848621002581-main.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.