Transcriptomics Analysis and Re-sequencing Reveal the Mechanism Underlying the Thermotolerance of an Artificial Selection Population of the Pacific Oyster

doi:10.3389/fphys.2021.663023

IOCAS-IR > 实验海洋生物学重点实验室

	Transcriptomics Analysis and Re-sequencing Reveal the Mechanism Underlying the Thermotolerance of an Artificial Selection Population of the Pacific Oyster
	Tan, Yulong 1,2,3; Cong, Rihao 2,3,4,5,6; Qi, Haigang 2,3,4,5,6; Wang, Luping 2,3,4,5,6; Zhang, Guofan 2,3,4,5,6; Pan, Ying 1; Li, Li 2,3,4,5,6
	2021-04-22
发表期刊	FRONTIERS IN PHYSIOLOGY
ISSN	1664-042X
卷号	12 页码:10
通讯作者	Pan, Ying(yingpan@gxu.edu.cn) ; Li, Li(lili@qdio.ac.cn)
摘要	The Pacific oyster is a globally important aquaculture species inhabiting the intertidal environment, which experiences great temperature variation. Mass deaths in the summer pose a major challenge for the oyster industry. We initiated an artificial selection breeding program in 2017 using acute heat shock treatments of the parents to select for thermotolerance in oysters. In this study, we compared the respiration rate, summer survival rate, gene expression, and gene structure of F-2 selected oysters and non-selected wild oysters. A transcriptional analysis revealed global divergence between the selected and control groups at the larval stage, including 4764 differentially expressed genes, among which 79 genes were heat-responsive genes. Five heat shock proteins were enriched, and four of the six genes (five heat stock genes in the enriched GO terms and KEGG pathways and BAG4) were differentially expressed in 1-year-old oysters. Integration of the transcriptomic and re-sequencing data of the selected and the control groups revealed 1090 genes that differentiated in both gene structure and expression. Two SNPs (single nucleotide polymorphism) that may mediate the expression of CGI_10022585 and CGI_10024709 were validated. In addition, the respiration rate of 1-year-old oysters varied significantly between the selected group and the control group at room temperature (20 degrees C). And the summer survival rate of the selected population was significantly improved. This study not only shows that artificial selection has a significant effect on the gene structure and expression of oysters, but it also helps reveal the mechanism underlying their tolerance of high temperature as well as the ability of oysters to adapt to climate change.
关键词	Pacific oyster artificial selection thermotolerance constitutive difference of gene expression gene structure
DOI	10.3389/fphys.2021.663023
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41876169] ; National Key R&D Program of China[2018YFD0900304] ; National Key R&D Program of China[2018YFD0901400] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2019Q06] ; Technology and the Modern Agro-Industry Technology Research System[CARS-49]
WOS研究方向	Physiology
WOS类目	Physiology
WOS记录号	WOS:000647438500001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170830
专题	实验海洋生物学重点实验室
通讯作者	Pan, Ying; Li, Li
作者单位	1.Guangxi Univ, Coll Anim Sci & Technol, Nanning, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, CAS, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.Pilot Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China 6.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Tan, Yulong,Cong, Rihao,Qi, Haigang,et al. Transcriptomics Analysis and Re-sequencing Reveal the Mechanism Underlying the Thermotolerance of an Artificial Selection Population of the Pacific Oyster[J]. FRONTIERS IN PHYSIOLOGY,2021,12:10.
APA	Tan, Yulong.,Cong, Rihao.,Qi, Haigang.,Wang, Luping.,Zhang, Guofan.,...&Li, Li.(2021).Transcriptomics Analysis and Re-sequencing Reveal the Mechanism Underlying the Thermotolerance of an Artificial Selection Population of the Pacific Oyster.FRONTIERS IN PHYSIOLOGY,12,10.
MLA	Tan, Yulong,et al."Transcriptomics Analysis and Re-sequencing Reveal the Mechanism Underlying the Thermotolerance of an Artificial Selection Population of the Pacific Oyster".FRONTIERS IN PHYSIOLOGY 12(2021):10.

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