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| A glimpse of deep-sea adaptation in chemosynthetic holobionts: Depressurization causes DNA fragmentation and cell death of methanotrophic endosymbionts rather than their deep-sea Bathymodiolinae host | |
Chen, Hao1,2,3 ; Wang, Minxiao1,2,3; Li, Mengna1,2,3,4; Lian, Chao1,2,3; Zhou, Li1,2,3; Zhang, Xin1,3; Zhang, Huan1,2,3; Zhong, Zhaoshan1,2,3; Wang, Hao1,2,3; Cao, Lei1,2,3; Li, Chaolun1,2,3,4
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| 2021-05-01 | |
| Source Publication | MOLECULAR ECOLOGY
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| ISSN | 0962-1083 |
| Volume | 30Issue:10Pages:2298-2312 |
| Corresponding Author | Li, Chaolun(lcl@qdio.ac.cn) |
| Abstract | Bathymodiolinae mussels are typical species in deep-sea cold seeps and hydrothermal vents and an ideal model for investigating chemosynthetic symbiosis and the influence of high hydrostatic pressure on deep-sea organisms. Herein, the potential influence of depressurization on DNA fragmentation and cell death in Bathymodiolinae hosts and their methanotrophic symbionts were surveyed using isobaric and unpressurized samples. As a hallmark of cell death, massive DNA fragmentation was observed in methanotrophic symbionts from unpressurized Bathymodiolinae while several endonucleases and restriction enzymes were upregulated. Additionally, genes involved in DNA repair, glucose/methane metabolism as well as two-component regulatory system were also differentially expressed in depressurized symbionts. DNA fragmentation and programmed cell death, however, were rarely detected in the host bacteriocytes owing to the orchestrated upregulation of inhibitor of apoptosis genes and downregulation of caspase genes. Meanwhile, diverse host immune recognition receptors were promoted during depressurization, probably enabling the regain of symbionts. When the holobionts were subjected to a prolonged acclimation at atmospheric pressure, alternations in both the DNA fragmentation and the expression atlas of aforesaid genes were continuously observed in symbionts, demonstrating the persistent influence of depressurization. Contrarily, the host cells demonstrated certain tolerance against depressurization stress as expression level of some immune-related genes returned to the basal level in isobaric samples. Altogether, the present study illustrates the distinct stress responses of Bathymodiolinae hosts and their methanotrophic symbionts against depressurization, which could provide further insight into the deep-sea adaptation of Bathymodiolinae holobionts while highlighting the necessity of using isobaric sampling methods in deep-sea research. |
| Keyword | bathymodiolinae mussel chemosynthetic symbiosis deep‐ sea adaptation hydrostatic pressure methane‐ oxidizing bacteria |
| DOI | 10.1111/mec.15904 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Key Research Program of Frontier Sciences, CAS[ZDBS-LY-DQC032] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050303] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42020401] ; National Key Research and Development Program of China[2018YFC0310800] ; National Natural Science Foundation of China[42030407] ; National Natural Science Foundation of China[42076091] ; National Natural Science Foundation of China[41906103] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, CAS[COMS2020Q02] ; Senior User Project of RV KEXUE[KEXUE2020G03] |
| WOS Research Area | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
| WOS Subject | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
| WOS ID | WOS:000639363800001 |
| Publisher | WILEY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/170613 |
| Collection | 海洋生态与环境科学重点实验室 |
| Corresponding Author | Li, Chaolun |
| Affiliation | 1.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences; Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences; Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Chen, Hao,Wang, Minxiao,Li, Mengna,et al. A glimpse of deep-sea adaptation in chemosynthetic holobionts: Depressurization causes DNA fragmentation and cell death of methanotrophic endosymbionts rather than their deep-sea Bathymodiolinae host[J]. MOLECULAR ECOLOGY,2021,30(10):2298-2312. |
| APA | Chen, Hao.,Wang, Minxiao.,Li, Mengna.,Lian, Chao.,Zhou, Li.,...&Li, Chaolun.(2021).A glimpse of deep-sea adaptation in chemosynthetic holobionts: Depressurization causes DNA fragmentation and cell death of methanotrophic endosymbionts rather than their deep-sea Bathymodiolinae host.MOLECULAR ECOLOGY,30(10),2298-2312. |
| MLA | Chen, Hao,et al."A glimpse of deep-sea adaptation in chemosynthetic holobionts: Depressurization causes DNA fragmentation and cell death of methanotrophic endosymbionts rather than their deep-sea Bathymodiolinae host".MOLECULAR ECOLOGY 30.10(2021):2298-2312. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| mec.15904.pdf（1857KB） | 期刊论文 | 出版稿 | 延迟开放 | CC BY-NC-SA | View 2023-7-1后可获取 | |
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