Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
Development of a high-resolution molecular marker for tracking Pseudo-nitzschia pungens genetic diversity through comparative analysis of mitochondrial genomes | |
Chen, Yang1,2,3,4![]() | |
2021-04-20 | |
Source Publication | JOURNAL OF APPLIED PHYCOLOGY
![]() |
ISSN | 0921-8971 |
Pages | 16 |
Abstract | The harmful algal bloom (HAB) species Pseudo-nitzschia pungens is widely distributed in almost all continents. Accumulating evidence suggests that P. pungens has high genetic diversity and many strains can produce the toxin domoic acid (DA) that harms animals and humans. Nevertheless, different P. pungens strains cannot be distinguished using morphological features or using common molecular markers including 18S rDNA, 28S rDNA, ITS, cox1, and rbcL. As such, high-resolution molecular markers need to be developed to resolve P. pungens genetic diversity, facilitating accurate tracking of toxic P. pungens strains. We hypothesized that molecular markers with high resolution and high specificity can be designed through identifying regions with high genomic variations in the mitochondrial genome. Here, we describe the development of a new molecular marker Pseudo-nitzschia pungens mitochondrial 1 (ppmt1) with high resolution and high specificity through comparative analysis of mitochondrial genomes of nine P. pungens strains isolated from coastal regions of China. In conclusion, we have developed ppmt1 as a high-resolution and high-specificity molecular marker for tracking strains and genetic diversity of the HAB species P. pungens. |
Keyword | Harmful algal bloom species Diatom Pseudo-nitzschia pungens Mitochondrial genome Comparative genomics Molecular marker |
DOI | 10.1007/s10811-021-02461-9 |
Indexed By | SCI |
Language | 英语 |
Funding Project | Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000] ; Chinese Academy of Sciences Pioneer Hundred Talents Program ; Taishan Scholar Project Special Fund ; Qingdao Innovation and Creation Plan (Talent Development Program-5th Annual Pioneer and Innovator Leadership Award)[19-3-2-16-zhc] ; Key Research Program of Frontier Sciences, Chinese Academy of Sciences[QYZDB-SSW-DQC023] ; Major Scientific and Technological Innovation Project of Shandong Province[2019JZZY020706] |
WOS Research Area | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
WOS Subject | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
WOS ID | WOS:000641637600001 |
Publisher | SPRINGER |
Citation statistics | |
Document Type | 期刊论文 |
Version | 出版稿 |
Identifier | http://ir.qdio.ac.cn/handle/337002/170598 |
Collection | 海洋生态与环境科学重点实验室 |
Corresponding Author | Chen, Nansheng |
Affiliation | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 3.Univ Chinese Acad Sci, Beijing 10039, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada |
First Author Affilication | Key Laboratory of Marine Ecology & Environmental Sciences, CAS |
Corresponding Author Affilication | Key Laboratory of Marine Ecology & Environmental Sciences, CAS |
Recommended Citation GB/T 7714 | Chen, Yang,Wang, Yichao,Liu, Kuiyan,et al. Development of a high-resolution molecular marker for tracking Pseudo-nitzschia pungens genetic diversity through comparative analysis of mitochondrial genomes[J]. JOURNAL OF APPLIED PHYCOLOGY,2021:16. |
APA | Chen, Yang,Wang, Yichao,Liu, Kuiyan,Liu, Feng,&Chen, Nansheng.(2021).Development of a high-resolution molecular marker for tracking Pseudo-nitzschia pungens genetic diversity through comparative analysis of mitochondrial genomes.JOURNAL OF APPLIED PHYCOLOGY,16. |
MLA | Chen, Yang,et al."Development of a high-resolution molecular marker for tracking Pseudo-nitzschia pungens genetic diversity through comparative analysis of mitochondrial genomes".JOURNAL OF APPLIED PHYCOLOGY (2021):16. |
Files in This Item: | ||||||
File Name/Size | DocType | Version | Access | License | ||
论文1.pdf(2086KB) | 期刊论文 | 作者接受稿 | 延迟开放 | CC BY-NC-SA | View 2023-7-1后可获取 |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment