IOCAS-IR  > 海洋生态与环境科学重点实验室
Development of a high-resolution molecular marker for tracking Pseudo-nitzschia pungens genetic diversity through comparative analysis of mitochondrial genomes
Chen, Yang1,2,3,4; Wang, Yichao1,2,3,4; Liu, Kuiyan1,2,3,4; Liu, Feng1,2,4; Chen, Nansheng1,2,4,5
2021-04-20
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
Pages16
Abstract

The harmful algal bloom (HAB) species Pseudo-nitzschia pungens is widely distributed in almost all continents. Accumulating evidence suggests that P. pungens has high genetic diversity and many strains can produce the toxin domoic acid (DA) that harms animals and humans. Nevertheless, different P. pungens strains cannot be distinguished using morphological features or using common molecular markers including 18S rDNA, 28S rDNA, ITS, cox1, and rbcL. As such, high-resolution molecular markers need to be developed to resolve P. pungens genetic diversity, facilitating accurate tracking of toxic P. pungens strains. We hypothesized that molecular markers with high resolution and high specificity can be designed through identifying regions with high genomic variations in the mitochondrial genome. Here, we describe the development of a new molecular marker Pseudo-nitzschia pungens mitochondrial 1 (ppmt1) with high resolution and high specificity through comparative analysis of mitochondrial genomes of nine P. pungens strains isolated from coastal regions of China. In conclusion, we have developed ppmt1 as a high-resolution and high-specificity molecular marker for tracking strains and genetic diversity of the HAB species P. pungens.

KeywordHarmful algal bloom species Diatom Pseudo-nitzschia pungens Mitochondrial genome Comparative genomics Molecular marker
DOI10.1007/s10811-021-02461-9
Indexed BySCI
Language英语
Funding ProjectStrategic Priority Research Program of Chinese Academy of Sciences[XDB42000000] ; Chinese Academy of Sciences Pioneer Hundred Talents Program ; Taishan Scholar Project Special Fund ; Qingdao Innovation and Creation Plan (Talent Development Program-5th Annual Pioneer and Innovator Leadership Award)[19-3-2-16-zhc] ; Key Research Program of Frontier Sciences, Chinese Academy of Sciences[QYZDB-SSW-DQC023] ; Major Scientific and Technological Innovation Project of Shandong Province[2019JZZY020706]
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000641637600001
PublisherSPRINGER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/170598
Collection海洋生态与环境科学重点实验室
Corresponding AuthorChen, Nansheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China
3.Univ Chinese Acad Sci, Beijing 10039, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
5.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada
First Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS
Corresponding Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS
Recommended Citation
GB/T 7714
Chen, Yang,Wang, Yichao,Liu, Kuiyan,et al. Development of a high-resolution molecular marker for tracking Pseudo-nitzschia pungens genetic diversity through comparative analysis of mitochondrial genomes[J]. JOURNAL OF APPLIED PHYCOLOGY,2021:16.
APA Chen, Yang,Wang, Yichao,Liu, Kuiyan,Liu, Feng,&Chen, Nansheng.(2021).Development of a high-resolution molecular marker for tracking Pseudo-nitzschia pungens genetic diversity through comparative analysis of mitochondrial genomes.JOURNAL OF APPLIED PHYCOLOGY,16.
MLA Chen, Yang,et al."Development of a high-resolution molecular marker for tracking Pseudo-nitzschia pungens genetic diversity through comparative analysis of mitochondrial genomes".JOURNAL OF APPLIED PHYCOLOGY (2021):16.
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