Effects of busulfan on somatic cells after inhibiting germ cells in the gonads of the young olive flounder Paralichthys olivaceus

doi:10.1016/j.anireprosci.2021.106746

IOCAS-IR > 实验海洋生物学重点实验室

	Effects of busulfan on somatic cells after inhibiting germ cells in the gonads of the young olive flounder Paralichthys olivaceus
	Wang, Wenxiang 1,2,3,4; Liang, Shaoshuai 1,2,3; Zou, Yuxia 1,2,3; Wang, Lijuan1,2,3 ; You, Feng 1,2,3
	2021-05-01
发表期刊	ANIMAL REPRODUCTION SCIENCE
ISSN	0378-4320
卷号	228 页码:10
通讯作者	You, Feng(youfeng@qdio.ac.cn)
摘要	Busulfan is widely used in some species to inhibit germ cell proliferation. This study was conducted to evaluate effects of busulfan on germ and somatic cells in gonads of olive flounder, Paralichthys olivaceus, one of the most economically important mariculture fish species. After intraperitoneal injection with 80 (80B) or 120 (120B) mg/kg busulfan, both gonads were atrophied, and ovaries were discolored with adhesion to the visceral mass. Histological results indicated that germ cells in the gonads were detached, and there was a larger nucleus size and smaller cytoplasmic volume in spermatogonia. Numbers of oocytes and somatic cells in the ovary were both less (P < 0.05), while in the testis, numbers of spermatogonia and somatic cells were markedly lesser and greater, respectively (P < 0.05). In ovaries of the flounder treated with 80B and 120B, relative abundance of vasa and cyp19a1a mRNA transcripts was very small in the cytoplasm of oocytes, while the cyp19a1a transcript was still present in theca cells. In the testis of flounder treated with 80B and 120B, abundance of vasa was markedly less (P < 0.05) with there being very little vasa in spermatogonia and disruption of the spermatogonium structure. In the 80B treatment group, amh was in lesser abundance with there being very little amh in spermatogonia, however, with the 120B treatment there was a large amh abundance in spermatogonium with there being disruption of structure of these germ cells and Sertoli cells. Busulfan, therefore, might inhibit the development of spermatogonia in the flounder testis.
关键词	Olive flounder Paralichthys olivaceus Busulfan Expression Spermatogonium disruption Gonadal somatic cell
DOI	10.1016/j.anireprosci.2021.106746
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31772834] ; National Natural Science Foundation of China[31872558] ; National Natural Science Foundation of China[31702337] ; National Key R&D Program of China[2018YFD0900202] ; National Marine Genetic Resource Center
WOS研究方向	Agriculture ; Reproductive Biology ; Veterinary Sciences
WOS类目	Agriculture, Dairy & Animal Science ; Reproductive Biology ; Veterinary Sciences
WOS记录号	WOS:000643550000007
出版者	ELSEVIER
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170592
专题	实验海洋生物学重点实验室
通讯作者	You, Feng
作者单位	1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Wang, Wenxiang,Liang, Shaoshuai,Zou, Yuxia,et al. Effects of busulfan on somatic cells after inhibiting germ cells in the gonads of the young olive flounder Paralichthys olivaceus[J]. ANIMAL REPRODUCTION SCIENCE,2021,228:10.
APA	Wang, Wenxiang,Liang, Shaoshuai,Zou, Yuxia,Wang, Lijuan,&You, Feng.(2021).Effects of busulfan on somatic cells after inhibiting germ cells in the gonads of the young olive flounder Paralichthys olivaceus.ANIMAL REPRODUCTION SCIENCE,228,10.
MLA	Wang, Wenxiang,et al."Effects of busulfan on somatic cells after inhibiting germ cells in the gonads of the young olive flounder Paralichthys olivaceus".ANIMAL REPRODUCTION SCIENCE 228(2021):10.

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