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Construction of a chromosome-level genome and variation map for the Pacific oyster Crassostrea gigas
Qi, Haigang1,2,3,5; Li, Li1,3,4,5; Zhang, Guofan1,2,3,5
2021-04-18
发表期刊MOLECULAR ECOLOGY RESOURCES
ISSN1755-098X
页码16
通讯作者Li, Li(lili@qdio.ac.cn) ; Zhang, Guofan(gfzhang@qdio.ac.cn)
摘要The Pacific oyster (Crassostrea gigas) is a widely distributed marine bivalve of great ecological and economic importance. In this study, we provide a high-quality chromosome-level genome assembled using Pacific Bioscience long reads and Hi-C-based and linkage-map-based scaffolding technologies and a high-resolution variation map constructed using large-scale resequencing analysis. The 586.8 Mb genome consists of 10 pseudochromosome sequences ranging from 38.6 to 78.9 Mb, containing 301 contigs with an N50 size of 3.1 Mb. A total of 30,078 protein-coding genes were predicted, of which 22,757 (75.7%) were high-reliability annotations supported by a homologous match to a curated protein in the SWISS-PROT database or transcript expression. Although a medium level of repeat components (57.2%) was detected, the genomic content of the segmental duplications reached 26.2%, which is the highest among the reported genomes. By whole genome resequencing analysis of 495 Pacific oysters, a comprehensive variation map was built, comprised of 4.78 million single nucleotide polymorphisms, 0.60 million short insertions and deletions, and 49,333 copy number variation regions. The structural variations can lead to an average interindividual genomic divergence of 0.21, indicating their crucial role in shaping the Pacific oyster genome diversity. The large amount of mosaic distributed repeat elements, small variations, and copy number variations indicate that the Pacific oyster is a diploid organism with an extremely high genomic complexity at the intra- and interindividual level. The genome and variation maps can improve our understanding of oyster genome diversity and enrich the resources for oyster molecular evolution, comparative genomics, and genetic research.
关键词Crassostrea gigas genome Pacific oyster resequencing variation
DOI10.1111/1755-0998.13368
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[41876169] ; Major scientific and technological innovation projects in Shandong Province[2019JZZY010813] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese academy of science[COMS2019Q06] ; Earmarked Fund for China Agriculture Research System[CARS-49]
WOS研究方向Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS类目Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS记录号WOS:000640876200001
出版者WILEY
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/170499
专题实验海洋生物学重点实验室
通讯作者Li, Li; Zhang, Guofan
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China
5.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China
第一作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
通讯作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
推荐引用方式
GB/T 7714
Qi, Haigang,Li, Li,Zhang, Guofan. Construction of a chromosome-level genome and variation map for the Pacific oyster Crassostrea gigas[J]. MOLECULAR ECOLOGY RESOURCES,2021:16.
APA Qi, Haigang,Li, Li,&Zhang, Guofan.(2021).Construction of a chromosome-level genome and variation map for the Pacific oyster Crassostrea gigas.MOLECULAR ECOLOGY RESOURCES,16.
MLA Qi, Haigang,et al."Construction of a chromosome-level genome and variation map for the Pacific oyster Crassostrea gigas".MOLECULAR ECOLOGY RESOURCES (2021):16.
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