Accumulation of different metals in oyster Crassostrea gigas: Significance and specificity of SLC39A (ZIP) and SLC30A (ZnT) gene families and polymorphism variation

doi:10.1016/j.envpol.2021.116706

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	Accumulation of different metals in oyster Crassostrea gigas: Significance and specificity of SLC39A (ZIP) and SLC30A (ZnT) gene families and polymorphism variation
	Meng, Jie1,2,6 ; Wang, Wen-Xiong 3,4,5; Li, Li 1,6; Zhang, Guofan 1,2,6
	2021-05-01
发表期刊	ENVIRONMENTAL POLLUTION
ISSN	0269-7491
卷号	276 页码:13
通讯作者	Zhang, Guofan(gfzhang@qdio.ac.cn)
摘要	The Zrt/Irt-like proteins (ZIP, SLC39A) and zinc transporters (ZnT, SLC30A) are the two major gene families responsible for the import/export of Zn and other metals. In this study, the mRNA expression levels and genetic variations of eight ZnTs and 14 ZIPs were identified in Crassostrea gigas after exposure to Zn, Cd, Cu, Hg, and Pb. Metal exposure induced reactive oxygen species (ROS) and malondialdehyde (MDA) accumulation and antioxidant enzyme expression. The expanded gene numbers of superoxide dismutase (SOD) in the oysters exhibited diverse expression under exposure to the five metals, and the contrasting expressions of both ZnTs and ZIPs under different metal exposures were observed, revealing their ion-specific responses. Zn and Cu have similar transporters and induce high expression levels of ZnT1, 2, 7, and 9 and ZIP1, 3, 6, 9, 10, 11, and 14. Pb induced high expression levels of ZIP7, and 13 and ZnT5, 6, and 7, which are mainly expressed in the endoplasmic reticulum (ER). Cd induced high expression levels of ZnT1, 2, and 7 and ZIP1, 6, 9,10,11, and 13. Hg exposure was found to have little effect on the ZIP and ZnT expression levels. Based on 3784 single nucleotide polymorphisms (SNPs) within the ZnTs and ZIPs, genetic association analysis for Zn accumulation was conducted on 427 oyster samples. The 38 SNPs, which were located within 12 genes, were identified to be associated with Zn content (p < 0.01), explaining the phenotypic variation from 1.61% to 3.37%. One nonsynonymous mutation and related haplotypes were identified within ZIP1, explaining 1.69% of the variation in Zn. Its high expression under Zn exposure revealed its important role in Zn transportation. To the best of our knowledge, this study is the first comprehensive investigation of the transportation mechanisms of ZIPs and ZnTs under different metal exposures and the genetic effect of Zn accumulation in oysters, and provides valuable biomarkers and genetic resources to evaluate environmental metal pollution. (C) 2021 Elsevier Ltd. All rights reserved.
关键词	Oyster Metals Antioxidant enzyme Zinc transporter Genetic variation
DOI	10.1016/j.envpol.2021.116706
收录类别	SCI
语种	英语
资助项目	Youth Innovation Promotion Association, Chinese Academy of Science (CAS)[2019210] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; National Natural Science Foundation of China[31530079] ; Shandong Provincial Natural Science Foundation of China[ZR2016DQ13] ; Earmarked Fund for Modern Agroindustry Technology Research System[CARS-49]
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000630774100045
出版者	ELSEVIER SCI LTD
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170384
专题	实验海洋生物学重点实验室
通讯作者	Zhang, Guofan
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.City Univ Hong Kong, Sch Energy & Environm, Kowloon, Hong Kong, Peoples R China 4.City Univ Hong Kong, State Key Lab Marine Pollut, Kowloon, Hong Kong, Peoples R China 5.City Univ Hong Kong, Res Ctr Oceans & Human Hlth, Shenzhen Res Inst, Shenzhen 518057, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Meng, Jie,Wang, Wen-Xiong,Li, Li,et al. Accumulation of different metals in oyster Crassostrea gigas: Significance and specificity of SLC39A (ZIP) and SLC30A (ZnT) gene families and polymorphism variation[J]. ENVIRONMENTAL POLLUTION,2021,276:13.
APA	Meng, Jie,Wang, Wen-Xiong,Li, Li,&Zhang, Guofan.(2021).Accumulation of different metals in oyster Crassostrea gigas: Significance and specificity of SLC39A (ZIP) and SLC30A (ZnT) gene families and polymorphism variation.ENVIRONMENTAL POLLUTION,276,13.
MLA	Meng, Jie,et al."Accumulation of different metals in oyster Crassostrea gigas: Significance and specificity of SLC39A (ZIP) and SLC30A (ZnT) gene families and polymorphism variation".ENVIRONMENTAL POLLUTION 276(2021):13.

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