IOCAS-IR  > 实验海洋生物学重点实验室
Accumulation of different metals in oyster Crassostrea gigas: Significance and specificity of SLC39A (ZIP) and SLC30A (ZnT) gene families and polymorphism variation
Meng, Jie1,2,6; Wang, Wen-Xiong3,4,5; Li, Li1,6; Zhang, Guofan1,2,6
2021-05-01
Source PublicationENVIRONMENTAL POLLUTION
ISSN0269-7491
Volume276Pages:13
Corresponding AuthorZhang, Guofan(gfzhang@qdio.ac.cn)
AbstractThe Zrt/Irt-like proteins (ZIP, SLC39A) and zinc transporters (ZnT, SLC30A) are the two major gene families responsible for the import/export of Zn and other metals. In this study, the mRNA expression levels and genetic variations of eight ZnTs and 14 ZIPs were identified in Crassostrea gigas after exposure to Zn, Cd, Cu, Hg, and Pb. Metal exposure induced reactive oxygen species (ROS) and malondialdehyde (MDA) accumulation and antioxidant enzyme expression. The expanded gene numbers of superoxide dismutase (SOD) in the oysters exhibited diverse expression under exposure to the five metals, and the contrasting expressions of both ZnTs and ZIPs under different metal exposures were observed, revealing their ion-specific responses. Zn and Cu have similar transporters and induce high expression levels of ZnT1, 2, 7, and 9 and ZIP1, 3, 6, 9, 10, 11, and 14. Pb induced high expression levels of ZIP7, and 13 and ZnT5, 6, and 7, which are mainly expressed in the endoplasmic reticulum (ER). Cd induced high expression levels of ZnT1, 2, and 7 and ZIP1, 6, 9,10,11, and 13. Hg exposure was found to have little effect on the ZIP and ZnT expression levels. Based on 3784 single nucleotide polymorphisms (SNPs) within the ZnTs and ZIPs, genetic association analysis for Zn accumulation was conducted on 427 oyster samples. The 38 SNPs, which were located within 12 genes, were identified to be associated with Zn content (p < 0.01), explaining the phenotypic variation from 1.61% to 3.37%. One nonsynonymous mutation and related haplotypes were identified within ZIP1, explaining 1.69% of the variation in Zn. Its high expression under Zn exposure revealed its important role in Zn transportation. To the best of our knowledge, this study is the first comprehensive investigation of the transportation mechanisms of ZIPs and ZnTs under different metal exposures and the genetic effect of Zn accumulation in oysters, and provides valuable biomarkers and genetic resources to evaluate environmental metal pollution. (C) 2021 Elsevier Ltd. All rights reserved.
KeywordOyster Metals Antioxidant enzyme Zinc transporter Genetic variation
DOI10.1016/j.envpol.2021.116706
Indexed BySCI
Language英语
Funding ProjectYouth Innovation Promotion Association, Chinese Academy of Science (CAS)[2019210] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; National Natural Science Foundation of China[31530079] ; Shandong Provincial Natural Science Foundation of China[ZR2016DQ13] ; Earmarked Fund for Modern Agroindustry Technology Research System[CARS-49]
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000630774100045
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/170384
Collection实验海洋生物学重点实验室
Corresponding AuthorZhang, Guofan
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.City Univ Hong Kong, Sch Energy & Environm, Kowloon, Hong Kong, Peoples R China
4.City Univ Hong Kong, State Key Lab Marine Pollut, Kowloon, Hong Kong, Peoples R China
5.City Univ Hong Kong, Res Ctr Oceans & Human Hlth, Shenzhen Res Inst, Shenzhen 518057, Peoples R China
6.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Meng, Jie,Wang, Wen-Xiong,Li, Li,et al. Accumulation of different metals in oyster Crassostrea gigas: Significance and specificity of SLC39A (ZIP) and SLC30A (ZnT) gene families and polymorphism variation[J]. ENVIRONMENTAL POLLUTION,2021,276:13.
APA Meng, Jie,Wang, Wen-Xiong,Li, Li,&Zhang, Guofan.(2021).Accumulation of different metals in oyster Crassostrea gigas: Significance and specificity of SLC39A (ZIP) and SLC30A (ZnT) gene families and polymorphism variation.ENVIRONMENTAL POLLUTION,276,13.
MLA Meng, Jie,et al."Accumulation of different metals in oyster Crassostrea gigas: Significance and specificity of SLC39A (ZIP) and SLC30A (ZnT) gene families and polymorphism variation".ENVIRONMENTAL POLLUTION 276(2021):13.
Files in This Item:
File Name/Size DocType Version Access License
1-s2.0-S026974912100(3162KB)期刊论文出版稿延迟开放CC BY-NC-SAView 2023-7-1后可获取
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Meng, Jie]'s Articles
[Wang, Wen-Xiong]'s Articles
[Li, Li]'s Articles
Baidu academic
Similar articles in Baidu academic
[Meng, Jie]'s Articles
[Wang, Wen-Xiong]'s Articles
[Li, Li]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Meng, Jie]'s Articles
[Wang, Wen-Xiong]'s Articles
[Li, Li]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 1-s2.0-S0269749121002852-main.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.