New insights into physiological effects of anoxia under darkness on the iconic seagrass Zostera marina based on a combined analysis of transcriptomics and metabolomics

doi:10.1016/j.scitotenv.2020.144717

	New insights into physiological effects of anoxia under darkness on the iconic seagrass Zostera marina based on a combined analysis of transcriptomics and metabolomics
	Zhang, Yu 1,2,3,4,5; Zhao, Peng6 ; Yue, Shidong 1,2,3,4,5; Liu, Mingjie 1,2,3,4,5; Qiao, Yongliang 1,2,3,4,7; Xu, Shaochun1,2,3,4,5 ; Gu, Ruiting 1,2,3,4,5; Zhang, Xiaomei1,2,3,4,5 ; Zhou, Yi1,2,3,4,5
	2021-05-10
发表期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
卷号	768 页码:11
通讯作者	Zhou, Yi(yizhou@qdio.ac.cn)
摘要	Coastal hypoxia/anoxia is a major emerging threat to global coastal ecosystems. Macroalgae blooms of tens of kilometers are often observed in open waters. These blooms not only cause a lack of oxygen, but also benthic light limitation. We explored the physiological responses of Zostera marina L to anoxia under darkness. After exposing Z. marina to anoxia under darkness for 72 h, we measured the elongation of leaves and the decrease in maximal quantum yield of photosystem II (Fv/Fm), and investigated the transcriptomic and metabolomic responses to anoxic stress based on RNA-sequencing and liquid chromatography-mass spectrometry (LC-MS) technology. The results showed that anoxic stress significantly reduced the leaf Fv/Fm, and had a significant negative effect on the photosynthesis and growth of Z. marina. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of up-regulated differentially expressed genes (DEGs) showed that glycolysis was the most significant enrichment pathway (p < 0.001), and most of the important products in glycolysis were significantly up-regulated. This indicated that the glycolysis process of anaerobic respiration is promoted under anoxia The metabolite results also showed that glyceraldehyde 3-phosphate in the glycolysis pathway was significantly up-regulated. Moreover, three genes encoding sucrose synthase (gene-ZOSMA_310G00150, gene-ZOSMA_81G00980, and gene-ZOSMA_8G00730) and one gene encoding alpha-amylase (gene-ZOSMA_95G00270) were significantly up-regulated, providing the sugar basis for the subsequent increase in glycolysis. Furthermore, gene-encoding oxoglutarate dehydrogenase, the rate-limiting step of the tricarboxylic add (TCA) cycle, was significantly down-regulated, indicating that this cycle was inhibited under anoxia. Metabolomic results showed that L-tryptophan, L-phenylalanine, and DL-leucine were significantly upregulated. Only significantly decreased glutamate and non-significantly decreased glutamine, substances consumed in alanine and gamma-aminobutyric acid (GABA) shunt mechanisms, were detected in the leaves, while GABA and alanine were not detected. The results of this study show that anoxic stress induces a programmed transcriptomic and metabolomic response in seagrass, most likely reflecting a complex strategy of acclimation and adaptation in seagrass to resist anoxic stress. (C) 2021 Elsevier B.V. All rights reserved.
关键词	Anoxia Physiological response Submerged aquatic vegetation Seagrass Photosynthesis and growth Transciiptomics Metabolomics Zostera marina L. Eelgrass
DOI	10.1016/j.scitotenv.2020.144717
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2019YFD0901300] ; National Natural Science Foundation of China[41606192] ; National Science & Technology Basic Work Program[2015FY110600] ; Key Research Project of Frontier Sciences of CAS[QYZDB-SSW-DQC041-1] ; Taishan Scholars Program (Distinguished Taishan Scholars)
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000625384700091
出版者	ELSEVIER
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170369
专题	海洋生态与环境科学重点实验室
通讯作者	Zhou, Yi
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China 7.Qingdao Univ Sci & Technol, Qingdao 266000, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心; 中国科学院海洋研究所
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心; 中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Yu,Zhao, Peng,Yue, Shidong,et al. New insights into physiological effects of anoxia under darkness on the iconic seagrass Zostera marina based on a combined analysis of transcriptomics and metabolomics[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2021,768:11.
APA	Zhang, Yu.,Zhao, Peng.,Yue, Shidong.,Liu, Mingjie.,Qiao, Yongliang.,...&Zhou, Yi.(2021).New insights into physiological effects of anoxia under darkness on the iconic seagrass Zostera marina based on a combined analysis of transcriptomics and metabolomics.SCIENCE OF THE TOTAL ENVIRONMENT,768,11.
MLA	Zhang, Yu,et al."New insights into physiological effects of anoxia under darkness on the iconic seagrass Zostera marina based on a combined analysis of transcriptomics and metabolomics".SCIENCE OF THE TOTAL ENVIRONMENT 768(2021):11.

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