Arginine Biosynthesis by a Bacterial Symbiont Enables Nitric Oxide Production and Facilitates Larval Settlement in the Marine-Sponge Host

doi:10.1016/j.cub.2020.10.051

	Arginine Biosynthesis by a Bacterial Symbiont Enables Nitric Oxide Production and Facilitates Larval Settlement in the Marine-Sponge Host
	Song, Hao 1,2,3,4; Hewitt, Olivia H.1; Degnan, Sandie M.1
	2021-01-25
发表期刊	CURRENT BIOLOGY
ISSN	0960-9822
卷号	31 期号:2 页码:433-+
通讯作者	Degnan, Sandie M.(s.degnan@uq.edu.au)
摘要	Larval settlement and metamorphosis are regulated by nitric oxide (NO) signaling in a wide diversity of marine invertebrates.(1-10) It is thus surprising that, in most invertebrates, the substrate for NO synthesis-arginine-cannot be biosynthesized but instead must be exogenously sourced.(11) In the sponge Amphimedon queenslandica, vertically inherited proteobacterial symbionts in the larva are able to biosynthesize arginine.(12,13) Here, we test the hypothesis that symbionts provide arginine to the sponge host so that nitric oxide synthase expressed in the larva can produce NO, which regulates metamorphosis,(8) and the byproduct citrulline (Figure 1). First, we find support for an arginine-citrulline biosynthetic loop in this sponge larval holobiont by using stable isotope tracing. In symbionts, incorporated C-13-citrulline decreases as C-13-arginine increases, consistent with the use of exogenous citrulline for arginine synthesis. In contrast, C-13-citrulline accumulates in larvae as C-13-arginine decreases, demonstrating the uptake of exogenous arginine and its conversion to NO and citrulline. Second, we show that, although Amphimedon larvae can derive arginine directly from seawater, normal settlement and metamorphosis can occur in artificial sea water lacking arginine. Together, these results support holobiont complementation of the arginine-citrulline loop and NO biosynthesis in Amphimedon larvae, suggesting a critical role for bacterial symbionts in the development of this marine sponge. Given that NO regulates settlement and metamorphosis in diverse animal phyla(1-10) and arginine is procured externally in most animals,(11) we propose that symbionts might play an equally critical regulatory role in this essential life cycle transition in other metazoans.
DOI	10.1016/j.cub.2020.10.051
收录类别	SCI
语种	英语
资助项目	Australian Research Council[DP110104601] ; Australian Research Council[DP170102353] ; Natural Science Foundation of Shandong Province[ZR2019BD003] ; China Postdoctoral Science Foundation[2019M652498] ; China Scholarship Council visiting scholar grant
WOS研究方向	Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Biology ; Cell Biology
WOS记录号	WOS:000631966000010
出版者	CELL PRESS
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170354
专题	海洋生态与环境科学重点实验室
通讯作者	Degnan, Sandie M.
作者单位	1.Univ Queensland, Sch Biol Sci, Brisbane, Qld 4072, Australia 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Song, Hao,Hewitt, Olivia H.,Degnan, Sandie M.. Arginine Biosynthesis by a Bacterial Symbiont Enables Nitric Oxide Production and Facilitates Larval Settlement in the Marine-Sponge Host[J]. CURRENT BIOLOGY,2021,31(2):433-+.
APA	Song, Hao,Hewitt, Olivia H.,&Degnan, Sandie M..(2021).Arginine Biosynthesis by a Bacterial Symbiont Enables Nitric Oxide Production and Facilitates Larval Settlement in the Marine-Sponge Host.CURRENT BIOLOGY,31(2),433-+.
MLA	Song, Hao,et al."Arginine Biosynthesis by a Bacterial Symbiont Enables Nitric Oxide Production and Facilitates Larval Settlement in the Marine-Sponge Host".CURRENT BIOLOGY 31.2(2021):433-+.

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