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C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian
Ma, Yuzhen1,2,3; Gao, Kun1,2,3,4; Yu, Huahua1,2,3; Liu, Weixiang1,2,3; Qin, Yukun1,2,3; Xing, Ronge1,2,3; Liu, Song1,2,3; Li, Pengcheng1,2,3
2021-06-01
Source PublicationCARBOHYDRATE POLYMERS
ISSN0144-8617
Volume261Pages:13
Corresponding AuthorYu, Huahua(yuhuahua@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
AbstractDamage to the cell membrane is an effective method to prevent drug resistance in plant fungal diseases. Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu). FITC-labeled O-CSLn-Cu (FITC-O-CSLn-Cu) was first synthesized via a nucleophilic substitution reaction and confirmed by FT-IR. FITC-labeled O-CSLn-Cu could pass through the fungal cell membrane, as detected by confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence. O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM). Then, we performed component analysis of the cell membrane from the P. capsici Leonian affected by OCSLn-Cu with a particular interest in membrane physicochemical properties. Many unsaturated fatty acids (UFAs) and key enzymes promoting UFA synthesis of the cell membrane were downregulated. Similarly, a large number of membrane proteins responsible for substance transport and biochemical reactions were downregulated. Furthermore, O-CSLn-Cu treatments increased plasma membrane permeability with significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation with decreasing membrane fluidity. Finally, aquaporin 10 was proven to be a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry.
KeywordNatural organic-based copper O-CSLn-Cu Phytophthora capsici Leonian Membrane damage and structural rearrangement Antifungal mechanism
DOI10.1016/j.carbpol.2021.117821
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFC0311300] ; National Key R&D Program of China[2019YFD0900705] ; National Natural Science Foundation of China[42076126]
WOS Research AreaChemistry ; Polymer Science
WOS SubjectChemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS IDWOS:000634108100006
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/170318
Collection实验海洋生物学重点实验室
Corresponding AuthorYu, Huahua; Li, Pengcheng
Affiliation1.Chinese Acad Sci, CAS, Qingdao, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Ma, Yuzhen,Gao, Kun,Yu, Huahua,et al. C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian[J]. CARBOHYDRATE POLYMERS,2021,261:13.
APA Ma, Yuzhen.,Gao, Kun.,Yu, Huahua.,Liu, Weixiang.,Qin, Yukun.,...&Li, Pengcheng.(2021).C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian.CARBOHYDRATE POLYMERS,261,13.
MLA Ma, Yuzhen,et al."C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian".CARBOHYDRATE POLYMERS 261(2021):13.
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