Chlororespiration protects the photosynthetic apparatus against photoinhibition by alleviating inhibition of photodamaged-PSII repair in Haematococcus pluvialis at the green motile stage

doi:10.1016/j.algal.2020.102140

IOCAS-IR > 实验海洋生物学重点实验室

	Chlororespiration protects the photosynthetic apparatus against photoinhibition by alleviating inhibition of photodamaged-PSII repair in Haematococcus pluvialis at the green motile stage
	Zhao, Jing 1,2; Yu, Wenjie 1,4; Zhang, Litao 1,3; Liu, Jianguo 1,3
	2021-04-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN	2211-9264
卷号	54 页码:8
通讯作者	Liu, Jianguo(jgliu@qdio.ac.cn)
摘要	During outdoor mass cultivation, Haematococcus pluvialis cells, especially at the green motile stage, are frequently exposed to high light. However, the photoprotective mechanisms in H. pluvialis have not been well characterized. This study aimed to confirm the photoprotection of chlororespiration in H. pluvialis at the green motile stage. Under high light, inhibition of plastid terminal oxidase (PTOX), a key enzyme of chlororespiration, increased the relative variable fluorescence at the I-step (VI), causing a decrease in the plastoquinone (PQ) pool, which further led to photosystem II (PSII) acceptor side over-reduction. This condition disturbed the balance between light absorption and energy utilization, causing the over-excitation of PSII reaction centers, which was indicated by the fact that the inhibition of PTOX increased the relative variable fluorescence at the J-step (V-J) and the light absorption flux per active reaction center (ABS/RC), but decreased the electron transport flux per active reaction center (ETo/RC) in H. pluvialis. The over-reduction of the PSII acceptor side and the over-excitation of PSII reaction centers inevitably caused more severe photoinhibition as shown by a decrease in F-v/Fm (maximal photochemical efficiency of PSII). Furthermore, the level of photoinhibition was not changed when the PTOX was inhibited in the presence of the photodamaged-PSII repair inhibitor chloramphenicol, indicating that the inhibition of PTOX did not accelerate the photodamage to PSII directly. The above results suggest that the chlororespiration pathway protects H. pluvialis at the green motile stage against photoinhibition by decreasing the inhibition of the photodamaged-PSII repair mechanism.
关键词	Chlororespiration Plastid terminal oxidase Photoprotection Haematococcus pluvialis
DOI	10.1016/j.algal.2020.102140
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2018YFE0107200] ; National Natural Science Foundation of China[31872588] ; National Natural Science Foundation of China[U1706209] ; Shandong Provincial Natural Science Foundation, China[ZR2019BC057]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000632338300006
出版者	ELSEVIER
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170303
专题	实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Zhao, Jing,Yu, Wenjie,Zhang, Litao,et al. Chlororespiration protects the photosynthetic apparatus against photoinhibition by alleviating inhibition of photodamaged-PSII repair in Haematococcus pluvialis at the green motile stage[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2021,54:8.
APA	Zhao, Jing,Yu, Wenjie,Zhang, Litao,&Liu, Jianguo.(2021).Chlororespiration protects the photosynthetic apparatus against photoinhibition by alleviating inhibition of photodamaged-PSII repair in Haematococcus pluvialis at the green motile stage.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,54,8.
MLA	Zhao, Jing,et al."Chlororespiration protects the photosynthetic apparatus against photoinhibition by alleviating inhibition of photodamaged-PSII repair in Haematococcus pluvialis at the green motile stage".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 54(2021):8.

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