Derivation and Characterization of a New Embryonic Cell Line from the Olive Flounder Paralichthys olivaceus

doi:10.4194/1303-2712-v21_4_01

IOCAS-IR > 实验海洋生物学重点实验室

	Derivation and Characterization of a New Embryonic Cell Line from the Olive Flounder Paralichthys olivaceus
	Nie, Miaomiao 1,2,3,4; Wu, Zhihao 1,3; You, Feng 1,3
	2021-04-01
发表期刊	TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
ISSN	1303-2712
卷号	21 期号:4 页码:159-167
通讯作者	You, Feng(youfeng@qdio.ac.cn)
摘要	A new embryonic cell line that consisted predominantly of fibroblast cells has been established from embryos at Kupffer's vesicle (KV) stage of the olive flounder Paralichthys olivaceus, designated as the PoEKC. PoEKC cells have been subcultured for 61 passages over a period of 18 months. The cells were cultured in DMEM/F-12 medium supplemented with antibiotics, MS, and growth factors at temperature of 25 degrees C. The growth curve of the PoEKC cells comprised an exponential growth phase and a long plateau phase. Chromosome analysis revealed that the cells possessed the normal flounder diploid karyotype of 2n = 48t. The origin of the cell line was confirmed by testing the partial sequences of cytochrome oxidase c subunit I (COI) gene of mtDNA. The pluripotency markers, genes OCT4-1, SOX2, KLF4, and NANOG showed positive signals in the PoEKC cells, and the cells also presented high ALP activity. According to the above results, the PoEKC cells might be pluripotency, though the pluripotency needs further confirmation. The cells were also successfully transfected with GFP reporter gene suggesting that it could be utilized for gene function study in the flounder.
关键词	Paralichthys olivaceus Embryonic cell culture Kupffer's vesicle
DOI	10.4194/1303-2712-v21_4_01
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31772834] ; National Natural Science Foundation of China[31602143] ; National Key R&D Program of China[2018YFD0900202] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000629251700001
出版者	CENTRAL FISHERIES RESEARCH INST
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170300
专题	实验海洋生物学重点实验室
通讯作者	You, Feng
作者单位	1.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Key Lab Expt Marine Biol,Ctr Ocean Megasci, Qingdao, Peoples R China 2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Nie, Miaomiao,Wu, Zhihao,You, Feng. Derivation and Characterization of a New Embryonic Cell Line from the Olive Flounder Paralichthys olivaceus[J]. TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES,2021,21(4):159-167.
APA	Nie, Miaomiao,Wu, Zhihao,&You, Feng.(2021).Derivation and Characterization of a New Embryonic Cell Line from the Olive Flounder Paralichthys olivaceus.TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES,21(4),159-167.
MLA	Nie, Miaomiao,et al."Derivation and Characterization of a New Embryonic Cell Line from the Olive Flounder Paralichthys olivaceus".TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES 21.4(2021):159-167.

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