Characterization of a Deep Sea Bacillus toyonensis Isolate: Genomic and Pathogenic Features

doi:10.3389/fcimb.2021.629116

IOCAS-IR > 实验海洋生物学重点实验室

	Characterization of a Deep Sea Bacillus toyonensis Isolate: Genomic and Pathogenic Features
	Luo, Jing-chang 1,2,3,4; Long, Hao5 ; Zhang, Jian 6; Zhao, Yan1,2,3 ; Sun, Li 1,2,3,4
	2021-03-10
发表期刊	FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
ISSN	2235-2988
卷号	11 页码:13
通讯作者	Sun, Li(lsun@qdio.ac.cn)
摘要	Bacillus toyonensis is a group of Gram-positive bacteria belonging to the Bacillus cereus group and used in some cases as probiotics or biocontrol agents. To our knowledge, B. toyonensis from the deep sea (depth >1,000 m) has not been documented. Here, we report the isolation and characterization of a B. toyonensis strain, P18, from a deep sea hydrothermal field. P18 is aerobic, motile, and able to grow at low temperatures (4 degrees C) and high concentrations of NaCl (8%). P18 possesses a circular chromosome of 5,250,895 bp and a plasmid of 536,892 bp, which encode 5,380 and 523 genes, respectively. Of these genes, 2,229 encode hypothetical proteins that could not be annotated based on the COG database. Comparative genomic analysis showed that P18 is most closely related to the type strain of B. toyonensis, BCT-7112(T). Compared to BCT-7112(T), P18 contains 1,401 unique genes, 441 of which were classified into 20 COG functional categories, and the remaining 960 genes could not be annotated. A total of 319 putative virulence genes were identified in P18, including toxin-related genes, and 24 of these genes are absent in BCT-7112(T). P18 exerted strong cytopathic effects on fish and mammalian cells that led to rapid cell death. When inoculated via injection into fish and mice, P18 rapidly disseminated in host tissues and induced acute infection and mortality. Histopathology revealed varying degrees of tissue lesions in the infected animals. Furthermore, P18 could survive in fish and mouse sera and possessed hemolytic activity. Taken together, these results provide the first evidence that virulent B. toyonensis exists in deep sea environments.
关键词	Bacillus toyonensis deep-sea hydrothermal vent virulence infection genome
DOI	10.3389/fcimb.2021.629116
收录类别	SCI
语种	英语
资助项目	Qingdao National Laboratory for Marine Science and Technology[QNLM2016 ORP0309] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050402] ; CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences[KLMVI-OP-202002] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Immunology ; Microbiology
WOS类目	Immunology ; Microbiology
WOS记录号	WOS:000631849600001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170124
专题	实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, CAS, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China 5.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou, Hainan, Peoples R China 6.Yan Tai Univ, Sch Ocean, Yantai, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Luo, Jing-chang,Long, Hao,Zhang, Jian,et al. Characterization of a Deep Sea Bacillus toyonensis Isolate: Genomic and Pathogenic Features[J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2021,11:13.
APA	Luo, Jing-chang,Long, Hao,Zhang, Jian,Zhao, Yan,&Sun, Li.(2021).Characterization of a Deep Sea Bacillus toyonensis Isolate: Genomic and Pathogenic Features.FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,11,13.
MLA	Luo, Jing-chang,et al."Characterization of a Deep Sea Bacillus toyonensis Isolate: Genomic and Pathogenic Features".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 11(2021):13.

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