A putative insulin receptor involved in immune response of Chinese mitten crab Eriocheir sinensis

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	A putative insulin receptor involved in immune response of Chinese mitten crab Eriocheir sinensis
	Wang, L.1,3 ; Chen, H.1 ; Qiu, L.1; Wang, L.1,2,4 ; Song, L.1,2,4
	2020
发表期刊	ISJ-INVERTEBRATE SURVIVAL JOURNAL
ISSN	1824-307X
卷号	18 页码:1-10
通讯作者	Song, L.(lshsong@dlou.edu.cn)
摘要	Insulin plays important roles in metabolic homeostasis during environmental challenges. The insulin receptor is a key molecule to receive and transduce insulin signals. In the present study, a novel insulin receptor was identified from the Chinese mitten crab Eriocheir sinensis (designated as EsIR). The coding region of EsIR gene was 3573 bp in length and encoded 1190 amino acids with all the functional domains of mammal insulin receptors, including furin-like domain, receptor L domain, transmembrane domain, and tyrosine kinase domain. Phylogenetic analysis showed that the EsIR shared the closest evolutionary relationship with the insulin receptor from Macrobrachium rosenbergii. Cell transfection experiments confirmed that EsIR proteins were localized on the cytomembrane. The mRNA transcripts of EsIR were widely distributed in various tissues with higher abundance in hepatopancreas and eyestalk of E. sinensis. After Aeromonas hydrophila stimulation, the expression level of EsIR mRNA decreased from 3 h to 6 h, and then increased at 12 h. The conserved structure and subcellular localization of EsIR together with its sensitivity to A. hydrophila stimulation implied that EsIR was probably involved in immune response of E. sinensis. The present study provided clues for the further investigation about the evolution and function of the insulin signaling pathway in invertebrates.
关键词	Aeromonas hydrophila infection chinese mitten crab insulin receptor immune response
收录类别	SCI
语种	英语
资助项目	National Key RD Program[2018YFD0900606] ; National Science Foundation of China[31530069] ; Fund for Outstanding Talents and Innovative Team of Agricultural Scientific Research ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Talented Scholars in Dalian Ocean University
WOS研究方向	Immunology ; Zoology
WOS类目	Immunology ; Zoology
WOS记录号	WOS:000614759900001
出版者	INVERTEBRATE SURVIVAL JOURNAL
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170106
专题	实验海洋生物学重点实验室
通讯作者	Song, L.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao Key Lab Marine Fish Breeding & Biotechnol, Qingdao 266071, Peoples R China 4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
第一作者单位	实验海洋生物学重点实验室
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wang, L.,Chen, H.,Qiu, L.,et al. A putative insulin receptor involved in immune response of Chinese mitten crab Eriocheir sinensis[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2020,18:1-10.
APA	Wang, L.,Chen, H.,Qiu, L.,Wang, L.,&Song, L..(2020).A putative insulin receptor involved in immune response of Chinese mitten crab Eriocheir sinensis.ISJ-INVERTEBRATE SURVIVAL JOURNAL,18,1-10.
MLA	Wang, L.,et al."A putative insulin receptor involved in immune response of Chinese mitten crab Eriocheir sinensis".ISJ-INVERTEBRATE SURVIVAL JOURNAL 18(2020):1-10.

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