Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus) | |
Liu, Yifan1,2,3,4; Liu, Qinghua2,4; Xu, Shihong2,3,4; Wang, Yanfeng2,3,4; Feng, Chengcheng2,3,4 ; Zhao, Chunyan2,3,4; Song, Zongcheng5; Li, Jun2,3,4
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| 2021-01-11 | |
| Source Publication | FRONTIERS IN MARINE SCIENCE
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| Volume | 7Pages:17 |
| Corresponding Author | Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn) |
| Abstract | Turbot (Scophthalmus maximus) is an important marine fish both in Europe and North China. Although there are plenty of studies on the reproduction of turbot, the complete cytological process of spermatogenesis remains unclear. In this study, we investigated the submicroscopic structure of total 23 types of male germ cells throughout the breeding season, with a relatively complete process of the primary spermatocytes. We found that the spermatid tail formed early at Spermatid II, and there were at least 16 spherical mitochondria in the spermatozoa. The hepatosomatic index (HSI) and gonadosomatic index (GSI) both peaked during the breeding season. Preliminary analysis showed that the vitality of mature sperm was negatively correlated with the proportion of sperm deformity. The serum 3,5,3 '-triiodothyronine (T3), 5-hydroxytryptamine (5-HT), testosterone (T), 17 alpha,20 beta-Dihydroxy-4-pregnen-3-one (17 alpha,20 beta-DHP), and 17 beta-estradiol (E2) all increased during the maturity period, with the change of T content most noticeable. Whereas in the testis, an overall high level of 11-ketotestosterone (11-KT) was more remarkable. The expression and localization of androgen receptor (AR) mRNA showed that the AR was highly expressed at the stages of II (15 - 70 g), with a slight rebound at the mature stages [IV(2200 g) to V(2500 g)], whose change was ahead to the changes of T and 11-KT. Fluorescence in situ hybridization (FISH) analysis showed that the AR mainly distributed in but not limited to Sertoli cells. This study represents the most complete overview of the reproductive cycle and spermatogenesis of turbot, which provides an important reference for the reproduction research and the guidance of flatfish breeding. |
| Keyword | fish reproduction spermatogenesis factory farmed fish steroids Scophthalmus maximus |
| DOI | 10.3389/fmars.2020.592880 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31702321] ; National Natural Science Foundation of China[31572602] ; National Natural Science Foundation of China[31802319] ; Zhejiang Provincial Natural Science Foundation of China[LY20C190008] ; Shandong Province Major Science and Technology Innovation Project[2018SDKJ0302-4] ; Shandong Province Key RD Projects[2019GHY112013] ; Natural Science Foundation of Shandong Province[ZR2018BC053] ; National Key Research and Development Program[2018YFD0901204] ; Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[GML2019ZD0402] ; Major Agricultural Application Technology Innovation Project of Shandong Province[SD2019YY011] ; Qingdao National Laboratory for Marine Science and Technology[2018SDKJ0502-2] ; China Agriculture Research System[CARS-47] ; Major Science and Technology for Scientific and Technological Innovation Projects (Shandong)[2019JZZY020710] ; STS Project[KFZD-SW-106] ; STS Project[ZSSD-019] ; STS Project[2017T3017] ; STS Project[2019T3022] |
| WOS Research Area | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| WOS Subject | Environmental Sciences ; Marine & Freshwater Biology |
| WOS ID | WOS:000611002200001 |
| Publisher | FRONTIERS MEDIA SA |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/170094 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Liu, Qinghua; Li, Jun |
| Affiliation | 1.Zhejiang Ocean Univ, Natl Engn Res Ctr Marine Aquaculture, Zhoushan, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Southern Marine Sci & Engn Guangdong Lab, Guangzhou, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Liu, Yifan,Liu, Qinghua,Xu, Shihong,et al. A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus)[J]. FRONTIERS IN MARINE SCIENCE,2021,7:17. |
| APA | Liu, Yifan.,Liu, Qinghua.,Xu, Shihong.,Wang, Yanfeng.,Feng, Chengcheng.,...&Li, Jun.(2021).A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus).FRONTIERS IN MARINE SCIENCE,7,17. |
| MLA | Liu, Yifan,et al."A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus)".FRONTIERS IN MARINE SCIENCE 7(2021):17. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| fmars-07-592880.pdf（3472KB） | 期刊论文 | 出版稿 | 延迟开放 | CC BY-NC-SA | View 2023-7-1后可获取 | |
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