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A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus)
Liu, Yifan1,2,3,4; Liu, Qinghua2,4; Xu, Shihong2,3,4; Wang, Yanfeng2,3,4; Feng, Chengcheng2,3,4; Zhao, Chunyan2,3,4; Song, Zongcheng5; Li, Jun2,3,4
2021-01-11
Source PublicationFRONTIERS IN MARINE SCIENCE
Volume7Pages:17
Corresponding AuthorLiu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
AbstractTurbot (Scophthalmus maximus) is an important marine fish both in Europe and North China. Although there are plenty of studies on the reproduction of turbot, the complete cytological process of spermatogenesis remains unclear. In this study, we investigated the submicroscopic structure of total 23 types of male germ cells throughout the breeding season, with a relatively complete process of the primary spermatocytes. We found that the spermatid tail formed early at Spermatid II, and there were at least 16 spherical mitochondria in the spermatozoa. The hepatosomatic index (HSI) and gonadosomatic index (GSI) both peaked during the breeding season. Preliminary analysis showed that the vitality of mature sperm was negatively correlated with the proportion of sperm deformity. The serum 3,5,3 '-triiodothyronine (T3), 5-hydroxytryptamine (5-HT), testosterone (T), 17 alpha,20 beta-Dihydroxy-4-pregnen-3-one (17 alpha,20 beta-DHP), and 17 beta-estradiol (E2) all increased during the maturity period, with the change of T content most noticeable. Whereas in the testis, an overall high level of 11-ketotestosterone (11-KT) was more remarkable. The expression and localization of androgen receptor (AR) mRNA showed that the AR was highly expressed at the stages of II (15 - 70 g), with a slight rebound at the mature stages [IV(2200 g) to V(2500 g)], whose change was ahead to the changes of T and 11-KT. Fluorescence in situ hybridization (FISH) analysis showed that the AR mainly distributed in but not limited to Sertoli cells. This study represents the most complete overview of the reproductive cycle and spermatogenesis of turbot, which provides an important reference for the reproduction research and the guidance of flatfish breeding.
Keywordfish reproduction spermatogenesis factory farmed fish steroids Scophthalmus maximus
DOI10.3389/fmars.2020.592880
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31702321] ; National Natural Science Foundation of China[31572602] ; National Natural Science Foundation of China[31802319] ; Zhejiang Provincial Natural Science Foundation of China[LY20C190008] ; Shandong Province Major Science and Technology Innovation Project[2018SDKJ0302-4] ; Shandong Province Key RD Projects[2019GHY112013] ; Natural Science Foundation of Shandong Province[ZR2018BC053] ; National Key Research and Development Program[2018YFD0901204] ; Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[GML2019ZD0402] ; Major Agricultural Application Technology Innovation Project of Shandong Province[SD2019YY011] ; Qingdao National Laboratory for Marine Science and Technology[2018SDKJ0502-2] ; China Agriculture Research System[CARS-47] ; Major Science and Technology for Scientific and Technological Innovation Projects (Shandong)[2019JZZY020710] ; STS Project[KFZD-SW-106] ; STS Project[ZSSD-019] ; STS Project[2017T3017] ; STS Project[2019T3022]
WOS Research AreaEnvironmental Sciences & Ecology ; Marine & Freshwater Biology
WOS SubjectEnvironmental Sciences ; Marine & Freshwater Biology
WOS IDWOS:000611002200001
PublisherFRONTIERS MEDIA SA
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/170094
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Qinghua; Li, Jun
Affiliation1.Zhejiang Ocean Univ, Natl Engn Res Ctr Marine Aquaculture, Zhoushan, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China
3.Southern Marine Sci & Engn Guangdong Lab, Guangzhou, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
5.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Yifan,Liu, Qinghua,Xu, Shihong,et al. A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus)[J]. FRONTIERS IN MARINE SCIENCE,2021,7:17.
APA Liu, Yifan.,Liu, Qinghua.,Xu, Shihong.,Wang, Yanfeng.,Feng, Chengcheng.,...&Li, Jun.(2021).A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus).FRONTIERS IN MARINE SCIENCE,7,17.
MLA Liu, Yifan,et al."A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus)".FRONTIERS IN MARINE SCIENCE 7(2021):17.
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