Proteomic analysis of hydrogen production in Chlorella pyrenoidosa under nitrogen deprivation

doi:10.1016/j.algal.2020.102143

IOCAS-IR > 实验海洋生物学重点实验室

	Proteomic analysis of hydrogen production in Chlorella pyrenoidosa under nitrogen deprivation
	Li, Ling 1,2; Zhang, Litao 1; Liu, Jianguo 1,2
	2021-03-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN	2211-9264
卷号	53 页码:9
通讯作者	Liu, Jianguo(jgliu@qdio.ac.cn)
摘要	Previously, Chlorella pyrenoidosa strain IOAC707S was found to produce hydrogen under nitrogen deprivation in seawater medium, without dark incubation, N-2 flushing or addition of uncoupler, which made it a candidate for clean and renewable energy production. To understand the molecular mechanism underlying hydrogen production by C. pyrenoidosa under nitrogen deprivation, iTRAQ-based proteomic analysis was conducted to investigate the differences in protein abundance under nitrogen deprivation (L1-N) compared with the control (CK). In total, 119 KEGG pathways were mapped for 2383 proteins, among which, the expression levels of most proteins involved in chlorophyll synthesis were down-regulated, while those involved in chlorophyll degradation were up-regulated in the L1-N culture; the expression level of serine glyoxylate aminotransferase involved in photorespiration was up-regulated 1.25 fold in the L1-N culture at 44 h, and the expression levels of most proteins involved in mitochondrial respiration were up-regulated in the L1-N culture. These results indicated a decrease of chlorophyll and an increase of respiration, which favored the establishment of anaerobiosis and hydrogen production.
关键词	Chlorella pyrenoidosa Hydrogen production Nitrogen deprivation Proteome
DOI	10.1016/j.algal.2020.102143
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41676142] ; National Natural Science Foundation of China[32072945] ; National Natural Science Foundation of China[31000114] ; Blue Life program of Qingdao National Laboratory for Marine Science and Technology[MS2019NO01]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000608813100021
出版者	ELSEVIER
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170014
专题	实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Li, Ling,Zhang, Litao,Liu, Jianguo. Proteomic analysis of hydrogen production in Chlorella pyrenoidosa under nitrogen deprivation[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2021,53:9.
APA	Li, Ling,Zhang, Litao,&Liu, Jianguo.(2021).Proteomic analysis of hydrogen production in Chlorella pyrenoidosa under nitrogen deprivation.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,53,9.
MLA	Li, Ling,et al."Proteomic analysis of hydrogen production in Chlorella pyrenoidosa under nitrogen deprivation".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 53(2021):9.

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