Susceptibility and immune responses of hybrid snakehead (Channa maculata female x Channa argus male) following infection with snakehead fish vesiculovirus

doi:10.1016/j.aquaculture.2020.736113

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	Susceptibility and immune responses of hybrid snakehead (Channa maculata female x Channa argus male) following infection with snakehead fish vesiculovirus
	Cao, Pan 1,3; Sun, Wei 2; Zhang, Yanbing 2; Zhou, Zicheng 2; Zhang, Xiaojun 2; Liu, Xiaodan 2
	2021-02-25
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	533 页码:10
通讯作者	Cao, Pan(at19890928@126.com) ; Liu, Xiaodan(liuxiaodan@yzu.edu.cn)
摘要	Snakehead fish vesiculovirus (SHVV) was initially isolated from diseased hybrid snakehead, and could cause great economic losses in snakehead culture due to its high morbidity and mortality. In this study, the susceptibility, histopathological analysis, and host immune response of hybrid snakehead infected with SHVV were investigated. SHVV was highly pathogenic to hybrid snakehead, and the infected fish exhibited typical signs of acute haemorrhages, and enlarged visceral organs. Histopathological analysis showed that the major tissues of diseased fish exhibited obvious inflammatory responses to SHVV infection. Additionally, humoral immune factors such as superoxide dismutase (SOD), catalase (CAT), and acid phosphatase (ACP) were assayed as serum indicators to evaluate the immune responses induced in infected hybrid snakehead. Quantitative real-time PCR (qRT-PCR) was also performed to evaluate the expression patterns of immune-related genes (HSP70, IL-8, MHCI alpha, MHCII alpha, SOD1, ZAP7 theta, PKCO, LCK, and HLA-A) in tissues of liver, spleen, head kidney and intestine. The results showed significantly enhanced immune activities in hybrid snakehead after SHVV infection. Altogether, these data provided insights into the pathogenic mechanism of SHVV and host immune response, facilitating the future prevention and treatment of hybrid snakehead infected with SHVV.
关键词	Hybrid snakehead Snakehead fish vesiculovirus Susceptibility Histopathological analysis Immune responses
DOI	10.1016/j.aquaculture.2020.736113
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[32002420] ; Natural Science Foundation of Jiangsu Province[BK20180915] ; Natural Science Foundation of Jiangsu Province[BK20190916] ; Yangzhou City-Yangzhou University Cooperation Foundation[YZ2019140] ; Natural Science Fund for College and Universities in Jiangsu Province[18KJB240003] ; Jiangsu Agriculture Science and Technology Innovation Found[CX(18)2012] ; 'Blue Project' of Yangzhou University
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000605010000007
出版者	ELSEVIER
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169896
专题	中国科学院海洋研究所
通讯作者	Cao, Pan; Liu, Xiaodan
作者单位	1.Yangzhou Univ, Inst Surface Engn, Yangzhou 225127, Jiangsu, Peoples R China 2.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Cao, Pan,Sun, Wei,Zhang, Yanbing,et al. Susceptibility and immune responses of hybrid snakehead (Channa maculata female x Channa argus male) following infection with snakehead fish vesiculovirus[J]. AQUACULTURE,2021,533:10.
APA	Cao, Pan,Sun, Wei,Zhang, Yanbing,Zhou, Zicheng,Zhang, Xiaojun,&Liu, Xiaodan.(2021).Susceptibility and immune responses of hybrid snakehead (Channa maculata female x Channa argus male) following infection with snakehead fish vesiculovirus.AQUACULTURE,533,10.
MLA	Cao, Pan,et al."Susceptibility and immune responses of hybrid snakehead (Channa maculata female x Channa argus male) following infection with snakehead fish vesiculovirus".AQUACULTURE 533(2021):10.

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