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Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas
Jia, Zhihao1,2; Jiang, Shuai2; Wang, Mengqiang2; Wang, Xiudan2; Liu, Yu1,3; Lv, Zhao2; Song, Xiaorui1,3; Li, Yiqun2; Wang, Lingling1,3,4; Song, Linsheng1,3,4,5
2021-02-12
Source PublicationFRONTIERS IN IMMUNOLOGY
ISSN1664-3224
Volume11Pages:15
Corresponding AuthorSong, Linsheng(lshsong@dlou.edu.cn)
AbstractDM9 refers to an uncharacterized protein domain that is originally discovered in Drosophila melanogaster. Two proteins with DM9 repeats have been recently identified from Pacific oyster Crassostrea gigas as mannose-specific binding pattern-recognition receptors (PRRs). In the present study, a novel member of DM9 domain containing protein (designated as CgDM9CP-4) was identified from C. gigas. CgDM9CP-4, about 16 kDa with only two tandem DM9 domains, was highly enriched in hemocytes and gill. The transcripts level of CgDM9CP-4 in circulating hemocytes were decreased after LPS, PGN and Vibrio splendidus stimulations. The recombinant protein of CgDM9CP-4 (rCgDM9CP-4) displayed a broad binding spectrum towards various pathogen-associated molecular patterns (PAMPs) (LPS, PGN, beta-glucan and Mannose) and microorganisms (Staphylococcus aureus, Micrococcus luteus, V. splendidus, V. anguillarum, Escherichia coli, Pichia pastoris and Yarrowia lipolytica). CgDM9CP-4 was mostly expressed in gill and some of the hemocytes. Flow cytometry analysis demonstrated that the CgDM9CP-4-positive hemocytes accounted for 7.3% of the total hemocytes, and they were small in size and less in granularity. CgDM9CP-4 was highly expressed in non-phagocytes (similar to 82% of total hemocytes). The reactive oxygen species (ROS) and the expression levels of cytokines in CgDM9CP-4-positive hemocytes were much lower than that in CgDM9CP-4-negative hemocytes. The mRNA expression level of CgDM9CP-4 in hemocytes was decreased after RNAi of hematopoietic-related factors (CgGATA, CgRunt, CgSCL, and CgNotch). In addition, CgDM9CP-4-positive cells were found to be much more abundant in hemocytes from gill than that from hemolymph, with most of them located in the gill filament. All these results suggested that CgDM9CP-4 was a novel member of PRR that expressed in undifferentiated pro-hemocytes to mediate immune recognition of pathogens.
KeywordCrassostrea gigas DM9 domain pattern recognition innate immune response pro-hemocytes
DOI10.3389/fimmu.2020.603270
Indexed BySCI
Language英语
Funding ProjectNational Key RD Program[2018YFD0900502] ; National Natural Science Foundation of China[U1706204] ; National Natural Science Foundation of China[41961124009] ; Outstanding Talents and Innovative teams of Agricultural Scientific Research and Earmarked Fund from Modern Agro-industry Technology Research System[CARS-49] ; Fund for Outstanding Talents and Innovative Team of Agricultural Scientific Research in Ministry of Agriculture ; Research Foundation for Distinguished Professor in Liaoning[XLYC1902012] ; Climbing Scholar in Liaoning
WOS Research AreaImmunology
WOS SubjectImmunology
WOS IDWOS:000621786800001
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/169759
Collection实验海洋生物学重点实验室
Corresponding AuthorSong, Linsheng
Affiliation1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China
5.Southern Lab Ocean Sci & Engn Guangdong, Zhuhai, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jia, Zhihao,Jiang, Shuai,Wang, Mengqiang,et al. Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas[J]. FRONTIERS IN IMMUNOLOGY,2021,11:15.
APA Jia, Zhihao.,Jiang, Shuai.,Wang, Mengqiang.,Wang, Xiudan.,Liu, Yu.,...&Song, Linsheng.(2021).Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas.FRONTIERS IN IMMUNOLOGY,11,15.
MLA Jia, Zhihao,et al."Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas".FRONTIERS IN IMMUNOLOGY 11(2021):15.
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