Genome-wide identification of chitinase genes in Thalassiosira pseudonana and analysis of their expression under abiotic stresses

doi:10.1186/s12870-021-02849-2

IOCAS-IR > 实验海洋生物学重点实验室

	Genome-wide identification of chitinase genes in Thalassiosira pseudonana and analysis of their expression under abiotic stresses
	Cheng, Haomiao 1,2,3; Shao, Zhanru 1,2; Lu, Chang 1,2,3; Duan, Delin 1,2,4
	2021-02-10
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	21 期号:1 页码:14
通讯作者	Shao, Zhanru(zrshao@qdio.ac.cn) ; Duan, Delin(dlduan@qdio.ac.cn)
摘要	BackgroundThe nitrogen-containing polysaccharide chitin is the second most abundant biopolymer on earth and is found in the cell walls of diatoms, where it serves as a scaffold for biosilica deposition. Diatom chitin is an important source of carbon and nitrogen in the marine environment, but surprisingly little is known about basic chitinase metabolism in diatoms.ResultsHere, we identify and fully characterize 24 chitinase genes from the model centric diatom Thalassiosira pseudonana. We demonstrate that their expression is broadly upregulated under abiotic stresses, despite the fact that chitinase activity itself remains unchanged, and we discuss several explanations for this result. We also examine the potential transcriptional complexity of the intron-rich T. pseudonana chitinase genes and provide evidence for two separate tandem duplication events during their evolution.ConclusionsGiven the many applications of chitin and chitin derivatives in suture production, wound healing, drug delivery, and other processes, new insight into diatom chitin metabolism has both theoretical and practical value.
关键词	Abiotic stress Chitinase Enzymatic activity Gene expression Gene family Thalassiosira pseudonana
DOI	10.1186/s12870-021-02849-2
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41806175] ; National Key R&D Program of China[2018YFD0900106] ; Youth project of Marine Biology and Biotechnology Laboratory in Pilot National Laboratory for Marine Science and Technology Project[YQ2018NO06]
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000619672000001
出版者	BMC
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169689
专题	实验海洋生物学重点实验室
通讯作者	Shao, Zhanru; Duan, Delin
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Bright Moon Seaweed Grp Co Ltd, State Key Lab Bioact Seaweed Subst, Qingdao 266400, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Cheng, Haomiao,Shao, Zhanru,Lu, Chang,et al. Genome-wide identification of chitinase genes in Thalassiosira pseudonana and analysis of their expression under abiotic stresses[J]. BMC PLANT BIOLOGY,2021,21(1):14.
APA	Cheng, Haomiao,Shao, Zhanru,Lu, Chang,&Duan, Delin.(2021).Genome-wide identification of chitinase genes in Thalassiosira pseudonana and analysis of their expression under abiotic stresses.BMC PLANT BIOLOGY,21(1),14.
MLA	Cheng, Haomiao,et al."Genome-wide identification of chitinase genes in Thalassiosira pseudonana and analysis of their expression under abiotic stresses".BMC PLANT BIOLOGY 21.1(2021):14.

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