Oceanomicrobium pacificus gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from seawater of tropical western Pacific

doi:10.1007/s10482-021-01523-9

IOCAS-IR

	Oceanomicrobium pacificus gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from seawater of tropical western Pacific
	Dai, Dadong 1; Li, Yangjie 1; He, Wenxuan 2,3; Qin, Fang 2,3; Zheng, Jinshui 1; Sun, Ming 1; Zhang, Dechao 2,3,4
	2021-03-01
发表期刊	ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
ISSN	0003-6072
卷号	114 期号:3 页码:303-311
通讯作者	Sun, Ming(m98sun@mail.hzau.edu.cn) ; Zhang, Dechao(zhangdechao@qdio.ac.cn)
摘要	The Gram-stain-negative, aerobic, ovoid or rod-shaped bacterial strain, designated KN286(T), was isolated from seawater of tropical western Pacific. Growth occurred between 15 and 40 degrees C (optimally at 30-35 degrees C), pH 6-9 (optimally at 7.0) and in the presence of 0.5-5.0% (w/v) NaCl (optimally between 2.0 and 3.0%). Strain KN286(T) contained Q-10 as the major respiratory quinone. The polar lipid profile contained phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, three phospholipids, three glycolipids, and three unidentified polar lipids. The predominant cellular fatty acid was summed feature 8 (composed of C-18:1 omega 7c and/or C-18:1 omega 6c). Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain KN286(T) was a member of the family Rhodobacteraceae and formed a distinct lineage. Strain KN286(T) has a genome size of 3.25 Mbp and a G + C content of 65.0 mol%. It encoded with some genes for carbohydrate-active enzymes, such as GH20 (Glycoside Hydrolase Family 20) and PL1 (Polysaccharide Lyase Family 1) and did not encode with a set of genes for reduction of nitrate to nitrite (nitrate reductase gamma subunit, respiratory nitrate reductase alpha N-terminal and respiratory nitrate reductase beta C-terminal). Based on phylogenetic analyses with single-copy orthologous clusters, low isDDH value (19.6%), low ANI (72.4%) and low AAI (65.7%) results, differential chemotaxonomic and physiological properties, strain KN286(T) represents a novel species of a novel genus of the family Rhodobacteraceae, for which the name Oceanomicrobium pacificus gen. nov., sp. nov. is proposed. The type strain of Oceanomicrobium pacificus is KN286(T) (=CGMCC 1.17118(T) = KCTC 72430(T)).
关键词	Rhodobacteraceae Pacific Ocean Taxonomy Comparative genomics Core genome
DOI	10.1007/s10482-021-01523-9
收录类别	SCI
语种	英语
资助项目	Science & Technology Basic Resources Investigation Program of China[2017FY100804] ; Science & Technology Basic Resources Investigation Program of China[KEXUE2019G09]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000615559200002
出版者	SPRINGER
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169659
专题	中国科学院海洋研究所
通讯作者	Sun, Ming; Zhang, Dechao
作者单位	1.Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Dai, Dadong,Li, Yangjie,He, Wenxuan,et al. Oceanomicrobium pacificus gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from seawater of tropical western Pacific[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2021,114(3):303-311.
APA	Dai, Dadong.,Li, Yangjie.,He, Wenxuan.,Qin, Fang.,Zheng, Jinshui.,...&Zhang, Dechao.(2021).Oceanomicrobium pacificus gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from seawater of tropical western Pacific.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,114(3),303-311.
MLA	Dai, Dadong,et al."Oceanomicrobium pacificus gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from seawater of tropical western Pacific".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 114.3(2021):303-311.

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