First Succinylome Profiling of Vibrio alginolyticus Reveals Key Role of Lysine Succinylation in Cellular Metabolism and Virulence

doi:10.3389/fcimb.2020.626574

IOCAS-IR > 实验海洋生物学重点实验室

	First Succinylome Profiling of Vibrio alginolyticus Reveals Key Role of Lysine Succinylation in Cellular Metabolism and Virulence
	Zeng, Fuyuan 1,2,3; Pang, Huanying 1,2,3,4; Chen, Ying 2,3; Zheng, Hongwei 2,3; Li, Wanxin 5; Ramanathan, Srinivasan 5; Hoare, Rowena 6; Monaghan, Sean J.6; Lin, Xiangmin 5; Jian, Jichang 1,2,3,4
	2021-02-05
发表期刊	FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
ISSN	2235-2988
卷号	10 页码:13
通讯作者	Pang, Huanying(phying1218@163.com)
摘要	Recent studies have shown that a key strategy of many pathogens is to use post-translational modification (PTMs) to modulate host factors critical for infection. Lysine succinylation (Ksuc) is a major PTM widespread in prokaryotic and eukaryotic cells, and is associated with the regulation of numerous important cellular processes. Vibrio alginolyticus is a common pathogen that causes serious disease problems in aquaculture. Here we used the affinity enrichment method with LC-MS/MS to report the first identification of 2082 lysine succinylation sites on 671 proteins in V. alginolyticus, and compared this with the lysine acetylation of V. alginolyticus in our previous work. The Ksuc modification of SodB and PEPCK proteins were further validated by Co-immunoprecipitation combined with Western blotting. Bioinformatics analysis showed that the identified lysine succinylated proteins are involved in various biological processes and central metabolism pathways. Moreover, a total of 1,005 (25.4%) succinyl sites on 502 (37.3%) proteins were also found to be acetylated, which indicated that an extensive crosstalk between acetylation and succinylation in V. alginolyticus occurs, especially in three central metabolic pathways: glycolysis/gluconeogenesis, TCA cycle, and pyruvate metabolism. Furthermore, we found at least 50 (7.45%) succinylated virulence factors, including LuxS, Tdh, SodB, PEPCK, ClpP, and the Sec system to play an important role in bacterial virulence. Taken together, this systematic analysis provides a basis for further study on the pathophysiological role of lysine succinylation in V. alginolyticus and provides targets for the development of attenuated vaccines.
关键词	Vibrio alginolyticus lysine succinylation acetylation crosstalk virulence factors
DOI	10.3389/fcimb.2020.626574
收录类别	SCI
语种	英语
资助项目	Shenzhen Science and Technology Project[JCYJ20190813104207152] ; Shenzhen Science and Technology Project[JCYJ20170818111629778] ; NSFC[32073015] ; NSFC[31670129] ; NSFC[31802343] ; Southern Marine Science and Engineering Guangdong Laboratory (Zhan jiang)[ZJW2019-06] ; China Postdoctoral Science Foundation[2019M662214]
WOS研究方向	Immunology ; Microbiology
WOS类目	Immunology ; Microbiology
WOS记录号	WOS:000619473900001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169656
专题	实验海洋生物学重点实验室
通讯作者	Pang, Huanying
作者单位	1.Guangdong Ocean Univ, Shenzhen Inst, Shenzhen, Peoples R China 2.Guangdong Ocean Univ, Fisheries Coll, Zhanjiang, Peoples R China 3.Southern Marine Sci & Engn Guangdong Lab Zhan Jia, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China 4.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China 5.Fujian Agr & Forestry Univ, Fujian Prov Key Lab Agroecol Proc & Safety Monito, Sch Life Sci, Fuzhou, Peoples R China 6.Univ Stirling, Inst Aquaculture, Stirling, Scotland
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zeng, Fuyuan,Pang, Huanying,Chen, Ying,et al. First Succinylome Profiling of Vibrio alginolyticus Reveals Key Role of Lysine Succinylation in Cellular Metabolism and Virulence[J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2021,10:13.
APA	Zeng, Fuyuan.,Pang, Huanying.,Chen, Ying.,Zheng, Hongwei.,Li, Wanxin.,...&Jian, Jichang.(2021).First Succinylome Profiling of Vibrio alginolyticus Reveals Key Role of Lysine Succinylation in Cellular Metabolism and Virulence.FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,10,13.
MLA	Zeng, Fuyuan,et al."First Succinylome Profiling of Vibrio alginolyticus Reveals Key Role of Lysine Succinylation in Cellular Metabolism and Virulence".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 10(2021):13.

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