DNA porn-porn nanostructure as a multifunctional platform for pathogenic bacteria determination and inactivation

doi:10.1016/j.bios.2021.112982

	DNA porn-porn nanostructure as a multifunctional platform for pathogenic bacteria determination and inactivation
	Zeng, Yan1,2,3 ; Qi, Peng1,2,3 ; Wang, Yingwen 1,2,3,4; Chen, Chao1,2,3 ; Zhang, Dun 1,2,3
	2021-04-01
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
卷号	177 页码:9
通讯作者	Qi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn)
摘要	Pathogenic bacteria levels are significantly related with disease control, clinical diagnosis, and even environmental monitoring. It is becoming highly urgent to achieve ultrasensitive detection of pathogenic bacteria and efficient combat of bacterial infection. Toward this end, we have assembled a DNA Pom-Pom nanostructure (PP-N) based multifunctional platform for pathogenic bacteria determination and inactivation. In particular, one DNA oligonucleotide probe that serve as a trigger was specifically designed for the autonomous cross-opening of metastable DNA hairpin probes and long dsDNA structure formation, achieving a catalytic self-assembly of DNA nanostructure. Numerous DNA strands in this PP-N assembly provide sufficient interaction sites for functional domains and connector, showing high programmability, excellent biostability, as well as selective target recognition. With these properties, the fluorescence dyes modified PP-N platform showed excellent bacteria analysis with both excellent selectivity and ultrasensitive determination limit as low as 2.0 CFU/mL. Furthermore, the aptamer-functionalized and antibiotics loaded PP-N platform demonstrate excellent merits of high antibiotics-loading capacity and negligible cytotoxicity to targets. Therefore, this DNA PP-N assembly based multifunctional platform promise its great application in targeted sensing, combating bacterial infection, and potential clinic therapy.
关键词	DNA nanostructure Pathogenic bacteria Ultrasensitive detection Bacteria inactivation Antibiotics loading
DOI	10.1016/j.bios.2021.112982
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41906037] ; National Natural Science Foundation of China[41876101] ; Young Elite Scientists Sponsorship Program by CAST[20180276]
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS类目	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS记录号	WOS:000618826100001
出版者	ELSEVIER ADVANCED TECHNOLOGY
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169607
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Qi, Peng; Zhang, Dun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Zeng, Yan,Qi, Peng,Wang, Yingwen,et al. DNA porn-porn nanostructure as a multifunctional platform for pathogenic bacteria determination and inactivation[J]. BIOSENSORS & BIOELECTRONICS,2021,177:9.
APA	Zeng, Yan,Qi, Peng,Wang, Yingwen,Chen, Chao,&Zhang, Dun.(2021).DNA porn-porn nanostructure as a multifunctional platform for pathogenic bacteria determination and inactivation.BIOSENSORS & BIOELECTRONICS,177,9.
MLA	Zeng, Yan,et al."DNA porn-porn nanostructure as a multifunctional platform for pathogenic bacteria determination and inactivation".BIOSENSORS & BIOELECTRONICS 177(2021):9.

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