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Proteoglycan from Bacillus sp. BS11 Inhibits the Inflammatory Response by Suppressing the MAPK and NF-kappa B Pathways in Lipopolysaccharide-Induced RAW264.7 Macrophages
Wang, Qingchi1,2,3,4; Liu, Weixiang1,2,3,4; Yue, Yang1,2,3,4; Sun, Chaomin1,2,3,4; Zhang, Quanbin1,2,3,4
2020-12-01
Source PublicationMARINE DRUGS
Volume18Issue:12Pages:16
Corresponding AuthorZhang, Quanbin(qbzhang@qdio.ac.cn)
AbstractInflammation is involved in the pathogenesis of many debilitating diseases. Proteoglycan isolated from marine Bacillus sp. BS11 (EPS11) was shown to have anticancer activity, but its anti-inflammatory potential remains elusive. In the present study, the anti-inflammatory effects and mechanism of EPS11 were evaluated using a lipopolysaccharide (LPS)-induced RAW264.7 macrophage model. Biochemical characterization showed that the total sugar content and protein content of EPS11 were 49.5% and 30.2% respectively. EPS11 was composed of mannose, glucosamine, galactosamine, glucose, galactose, rhamnose, and glucuronic acid. Its molecular weight was determined to be 3.06 x 10(5) Da. The protein determination of EPS11 was also performed. EPS11 displayed a strong anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages in vitro, which significantly suppressed inflammatory cytokines and mediators (such as NO, TNF-alpha, IL-6 and IL-1 beta, and COX-2). Western blot analysis indicated that EPS11 could downregulate the expression of many key proteins in mitogen-activated protein kinases (MAPKs) and transcription factor nuclear factor-kappa B (NF-kappa B) signaling pathways. In particular, EPS11 almost completely inhibited the expression of NF-kappa B P65, which indicated that EPS11 acted primarily on the NF-kappa B pathways. These findings offer new insights into the molecular mechanism underlying the anti-inflammatory effect of EPS11.
KeywordBacillus sp proteoglycan anti-inflammation macrophages
DOI10.3390/md18120585
Indexed BySCI
Language英语
Funding ProjectChina Ocean Mineral Resources RD Association[DY135-B2-14]
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal
WOS IDWOS:000602108800001
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/169421
Collection实验海洋生物学重点实验室
Corresponding AuthorZhang, Quanbin
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Dept Earth Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Qingchi,Liu, Weixiang,Yue, Yang,et al. Proteoglycan from Bacillus sp. BS11 Inhibits the Inflammatory Response by Suppressing the MAPK and NF-kappa B Pathways in Lipopolysaccharide-Induced RAW264.7 Macrophages[J]. MARINE DRUGS,2020,18(12):16.
APA Wang, Qingchi,Liu, Weixiang,Yue, Yang,Sun, Chaomin,&Zhang, Quanbin.(2020).Proteoglycan from Bacillus sp. BS11 Inhibits the Inflammatory Response by Suppressing the MAPK and NF-kappa B Pathways in Lipopolysaccharide-Induced RAW264.7 Macrophages.MARINE DRUGS,18(12),16.
MLA Wang, Qingchi,et al."Proteoglycan from Bacillus sp. BS11 Inhibits the Inflammatory Response by Suppressing the MAPK and NF-kappa B Pathways in Lipopolysaccharide-Induced RAW264.7 Macrophages".MARINE DRUGS 18.12(2020):16.
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