Development of a high-resolution molecular marker for tracking Phaeocystis globosa genetic diversity through comparative analysis of chloroplast genomes

doi:10.1016/j.hal.2020.101911

	Development of a high-resolution molecular marker for tracking Phaeocystis globosa genetic diversity through comparative analysis of chloroplast genomes
	Song, Huiyin 1,2,3; Liu, Feng 1,2,3; Li, Zelin 1,2,3,4; Xu, Qing 1,2,3,5; Chen, Yang1,2,3,6 ; Yu, Zhiming1,2 ; Chen, Nansheng 1,2,3,7
	2020-11-01
发表期刊	HARMFUL ALGAE
ISSN	1568-9883
卷号	99 页码:14
通讯作者	Chen, Nansheng(chenn@qdio.ac.cn)
摘要	The phytoplankton Phaeocystis globosa thrives in a wide range of marine regions and plays an important role in climate control. It can also form harmful algal blooms (HABs) that threaten environments and impact important coastal infrastructures. Mechanisms underlying the formation of P. globosa blooms still remain poorly understood. Accumulating evidence suggests that P. globosa has high genetic diversity and different P. globosa strains may have differential contributions to the development of P. globosa blooms. However, due to the lack of molecular markers with adequate resolution for distinguishing P. globosa genetic diversity, such differential contributions by different P. globosa strains could not be accurately ascertained. As such, high-resolution molecular markers need to be developed and applied to distinguish P. globosa genetic diversity. In this study, we undertook to define high-resolution molecular marker by assembling and comparing the whole chloroplast genomes of P. globosa strains isolated from different regions of the world. Through comparative analysis of P. globosa cpDNAs and detection of single nucleotide variations (SNVs), a molecular marker pgcp1 with improved resolution was developed. The pgcp1 demonstrated the highest resolution compared with other regions including 18S rDNA V4 region, 28S rDNA D1-D2 region and rbcL region, through genetic distance and phylogenetic analysis of 13 P. globosa strains. Molecular analysis of environmental samples and strains collected in multiple expeditions from a wide range of ocean regions including multiple regions in China, Vietnam, Thailand and Western Pacific using pgcp1 as the molecular marker displayed high genetic diversity of P. globosa with at least four major P. globosa clades. In conclusion, we have developed pgcp1 as a high-resolution molecular marker for the harmful algal bloom species P. globosa, which can be used to track intra-species genetic diversity and dynamics of P. globosa during bloom development.
关键词	Chloroplast genome Harmful algal bloom (HAB) Single nucleotide variations (SNVs) Intraspecific relationship Molecular marker
DOI	10.1016/j.hal.2020.101911
收录类别	SCI
语种	英语
资助项目	Qingdao National Laboratory for Marine Science and Technology[QNLM201704] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000] ; Chinese Academy of Sciences Pioneer Hundred Talents Program ; Taishan Scholar Project Special Fund ; Qingdao Innovation and Creation Plan (Talent Development Program -5th Annual Pioneer and Innovator Leadership Award)[19-3-2-16-zhc] ; Key Research Program of Frontier Sciences, Chinese Academy of Sciences[QYZDB-SSW-DQC023] ; Youth Talent Support Program of the Laboratory for Marine Ecology and Environmental Science ; Pilot National Laboratory for Marine Science and Technology (Qingdao)[LMEES-YTSP-2018-01-08] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0504]
WOS研究方向	Marine & Freshwater Biology
WOS类目	Marine & Freshwater Biology
WOS记录号	WOS:000592393300006
出版者	ELSEVIER
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169259
专题	海洋生态与环境科学重点实验室
通讯作者	Chen, Nansheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Ocean Univ China, Qingdao 266100, Peoples R China 5.Huazhong Agr Univ, Wuhan 430070, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Song, Huiyin,Liu, Feng,Li, Zelin,et al. Development of a high-resolution molecular marker for tracking Phaeocystis globosa genetic diversity through comparative analysis of chloroplast genomes[J]. HARMFUL ALGAE,2020,99:14.
APA	Song, Huiyin.,Liu, Feng.,Li, Zelin.,Xu, Qing.,Chen, Yang.,...&Chen, Nansheng.(2020).Development of a high-resolution molecular marker for tracking Phaeocystis globosa genetic diversity through comparative analysis of chloroplast genomes.HARMFUL ALGAE,99,14.
MLA	Song, Huiyin,et al."Development of a high-resolution molecular marker for tracking Phaeocystis globosa genetic diversity through comparative analysis of chloroplast genomes".HARMFUL ALGAE 99(2020):14.

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