Comparative Transcriptome Analysis of Body-Color Segregated Progenies Produced by Crossing Albino Sea Cucumbers Apostichopus japonicus

doi:10.17957/IJAB/15.1515

	Comparative Transcriptome Analysis of Body-Color Segregated Progenies Produced by Crossing Albino Sea Cucumbers Apostichopus japonicus
	Ma, Deyou 1,2; Wu, Chunying 1; Yu, Lianlian 1; Zhang, Chanchan 1; Lin, Weigang 1; Yang, Hongsheng2
	2020
发表期刊	INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY
ISSN	1560-8530
卷号	24 期号:4 页码:900-908
通讯作者	Yang, Hongsheng(hshyang@qdio.ac.cn)
摘要	Body color is an economic trait of Apostichopus japonicus with growing concern. Among the other color variants (green and purple), rare sea cucumbers with completely white body color is greatly appealing to the consumers. In this study, comparative transcriptome analysis of body wall from body-color segregated progenies produced by cross of albino sea cucumber bmodstock was performed using RNA-Seq technology in order to profile dynamic characteristics of genome-wide gene expression. Over 4.78 and 4.87 million clean reads were generated from the segregated and albino juveniles crossed by albino parent sea cucumbers. Totally 20,319 assembled unigenes with the average 959.83 bp in length were obtained, including the annotated 7,152. Compared with white juvenile sea cucumbers, 271 genes were validated to be significantly differentially expressed in the body wall of segregated samples. Forty-two differentially expressed genes (DEGs) including 31 upregulated and 11 downregulated were identified presenting 5 and more fold expression changes, 18 of which were involved in growth and development, immunity and respiration. "Cell and cell part", "Cellular process" and "Binding" were the most DEG-enriched GO terms in "Cell component", "Biological process" and "Molecular function", respectively. DEGs were significantly enriched in immunity-related KEGG pathways, such as "Phagosome", "Staphylococcus aureus infection", "Complement and coagulation cascades", and "Vibrio cholerae infection". These results provided abundant candidate genes and pathways to further reveal differences in growth and immunity between segregated and white progenies crossed from albino A. japonicus, for cultivating genetically stable white strain of this commercially important species. (C) 2020 Friends Science Publishers
关键词	Apostichopus japonicus Albino progenies Body color segregation Transcriptome analysis RNA-Seq
DOI	10.17957/IJAB/15.1515
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2019YFD0900405] ; National Natural Science Foundation of China[41706180] ; Dalian Supporting High-Level Talent Innovation and Entrepreneurship Program[2017RQ031]
WOS研究方向	Agriculture ; Life Sciences & Biomedicine - Other Topics
WOS类目	Agriculture, Multidisciplinary ; Biology
WOS记录号	WOS:000591191100034
出版者	FRIENDS SCIENCE PUBL
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169187
专题	海洋生态与环境科学重点实验室
通讯作者	Yang, Hongsheng
作者单位	1.Dalian Ocean Univ, Minist Agr & Rural Affairs, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Ma, Deyou,Wu, Chunying,Yu, Lianlian,et al. Comparative Transcriptome Analysis of Body-Color Segregated Progenies Produced by Crossing Albino Sea Cucumbers Apostichopus japonicus[J]. INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY,2020,24(4):900-908.
APA	Ma, Deyou,Wu, Chunying,Yu, Lianlian,Zhang, Chanchan,Lin, Weigang,&Yang, Hongsheng.(2020).Comparative Transcriptome Analysis of Body-Color Segregated Progenies Produced by Crossing Albino Sea Cucumbers Apostichopus japonicus.INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY,24(4),900-908.
MLA	Ma, Deyou,et al."Comparative Transcriptome Analysis of Body-Color Segregated Progenies Produced by Crossing Albino Sea Cucumbers Apostichopus japonicus".INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY 24.4(2020):900-908.