The Effect of Pedal Peptide-Type Neuropeptide on Locomotor Behavior and Muscle Physiology in the Sea Cucumber Apostichopus japonicus

doi:10.3389/fphys.2020.559348

	The Effect of Pedal Peptide-Type Neuropeptide on Locomotor Behavior and Muscle Physiology in the Sea Cucumber Apostichopus japonicus
	Ding, Kui1,2,3,4 ; Zhang, Libin 1,2,3,4,5,6; Fan, Xinhao 1,2,3; Guo, Xueying 1,2,3; Liu, Xiang 1,2,3; Yang, Hongsheng1,2,3,4,5,7
	2020-10-22
发表期刊	FRONTIERS IN PHYSIOLOGY
ISSN	1664-042X
卷号	11 页码:11
通讯作者	Zhang, Libin(zhanglibin@qdio.ac.cn)
摘要	Neuropeptides are endogenous active substances that are present in nervous tissues and participate in behavioral and physiological processes of the animal system. Locomotor behavior is basic to predation, escape, reproduction in animals, and neuropeptides play an important role in locomotion. In this study, the function of pedal peptide-type neuropeptide (PDP) in the process of locomotor behavior of the sea cucumber Apostichopus japonicus was evaluated. The locomotor behavior of A. japonicus was recorded by infrared camera before and after PDP administration, and muscle physiology was studied by ultra performance liquid chromatography and quadrupole time-off-light mass spectrometry (UPLC-Q-TOF-MS) to clarify the potential physiological mechanisms. The results showed that PDP enhanced the cumulative duration of moving significantly at the 7th h after injection, and reduced the mean and maximum velocity by 16.90 and 14.22% in A. japonicus. The data of muscle metabolomics suggested that some significantly changed metabolites were related to locomotor behavior of sea cucumbers. The decreases of phosphatidylethanolamine (PE) and phosphatidylcholine (PC) might result in the increases of lysophosphatidylcholines (lysoPC) and lysophosphatidylethanolamine (lysoPE), and suggested the change of fluidity and permeability in the muscle cell membrane, which would affect the physiology and function of muscle cells, and finally alter the locomotor behavior. In addition, the increased level of arachidonic acid (ARA) might activate K+ ion channels and then affect the signaling of muscle cells, or promote the sensitivity of muscle cells to Ca2+ and then result in the contractility of longitudinal muscles in sea cucumbers. ARA was also involved in the linoleic acid metabolism which was the only pathway that disturbed significantly after PDP administration. In conclusion, PDP participated in the regulation of locomotor behavior in the sea cucumber, and the decreased PE and PC, increased lysoPC, lysoPE and ARA might be the potential physiological mechanisms that responsible for behavioral effects of PDP in A. japonicus.
关键词	echinoderm locomotor performance muscle metabolite pedal peptide neuropeptide
DOI	10.3389/fphys.2020.559348
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41876157] ; National Natural Science Foundation of China[41676136] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2019Q15] ; STS Program of Chinese Academy of Sciences[KFJ-STS-ZDTP-077] ; Taishan Scholars Program (Distinguished Taishan Scholars)
WOS研究方向	Physiology
WOS类目	Physiology
WOS记录号	WOS:000585194800001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169139
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Libin
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.Minist Nat Resources, Inst Oceanog 2, State Key Lab Satellite Ocean Environm Dynam, Hangzhou, Peoples R China 7.Chinese Acad Sci, Innovat Acad Seed Design, Wuhan, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Ding, Kui,Zhang, Libin,Fan, Xinhao,et al. The Effect of Pedal Peptide-Type Neuropeptide on Locomotor Behavior and Muscle Physiology in the Sea Cucumber Apostichopus japonicus[J]. FRONTIERS IN PHYSIOLOGY,2020,11:11.
APA	Ding, Kui,Zhang, Libin,Fan, Xinhao,Guo, Xueying,Liu, Xiang,&Yang, Hongsheng.(2020).The Effect of Pedal Peptide-Type Neuropeptide on Locomotor Behavior and Muscle Physiology in the Sea Cucumber Apostichopus japonicus.FRONTIERS IN PHYSIOLOGY,11,11.
MLA	Ding, Kui,et al."The Effect of Pedal Peptide-Type Neuropeptide on Locomotor Behavior and Muscle Physiology in the Sea Cucumber Apostichopus japonicus".FRONTIERS IN PHYSIOLOGY 11(2020):11.

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