IOCAS-IR  > 海洋生态与环境科学重点实验室
Snapshot of Peptidomics of the Red Tide Forming Species Noctiluca scintillans
Zhang, Shuwen1; Li, Cheng2; Cheung, Shunyan3; Sun, Mingming2; Song, Shuqun4; Guo, Wang2; Guo, Cui5,6; Wu, Gongguo2; Liu, Hongbin3,7
2020-10-27
Source PublicationFRONTIERS IN MARINE SCIENCE
Volume7Pages:11
Corresponding AuthorLiu, Hongbin(liuhb@ust.hk)
AbstractThe objective of this study was to investigate the peptidome of a red tide forming species Noctiluca scintillans based on mass-spectrometry (MS) analysis. N. scintilans cells were collected at the initiation and peak phases of its bloom. Peptides were extracted by ultrafiltration with cutoff of 10 and 3 kDa in acidic condition. Optimal mass spectra were generated after purification with C18 column. MS analysis and peptide identification was undertaken by Linear Trap Quadropole (LTQ) Velos MS/MS instrument. Using this method, 239 and 332 peptide sequences from the samples collected at the initiation and peak phases of N. scintillans bloom were identified, respectively. These sequences were classified and they were affiliated to 68 and 93 groups of precursor proteins, respectively, at the initiation and peak phases, with a compositional dissimilarity of 49.5%. Meanwhile, majority of the peptides were conjectured to come from contents digested by N. scintilans. Thirty-three groups of precursor proteins were detected at both phases. These peptides involved in various intracellular physiological activities, most of them responsible for functions of digestion activities such as oxidoreductase activity, catalytic activity, hydrolase activity and cofactor/ion binding. The compositions of precursor proteins, molecular weights, functions and cell compartments of the obtained peptides were quite different between two bloom stages. This is the first attempt to study peptidomics of N. scintillans, which provide important information on the functional studies of peptidomics in N. scintillans, especially food digestion and dietary protein utilization, as well as their relationship with bloom development.
KeywordNoctiluca scintillans peptidomics mass spectrometry protein food digestion
DOI10.3389/fmars.2020.569807
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41906117] ; Guangdong MEPP Fund[GDOE (2019) A23] ; Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[SMSEGL20SC01] ; Research Grants Council of Hong Kong[T21/602/16] ; Research Grants Council of Hong Kong[N_HKUST609/15] ; Research Grants Council of Hong Kong[16128416]
WOS Research AreaEnvironmental Sciences & Ecology ; Marine & Freshwater Biology
WOS SubjectEnvironmental Sciences ; Marine & Freshwater Biology
WOS IDWOS:000585733600001
PublisherFRONTIERS MEDIA SA
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/169112
Collection海洋生态与环境科学重点实验室
Corresponding AuthorLiu, Hongbin
Affiliation1.South China Normal Univ, Coll Life Sci, Guangdong Prov Key Lab Healthy & Safe Aquaculture, Guangzhou, Peoples R China
2.Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China
3.Hong Kong Univ Sci & Technol, Dept Ocean Sci, Kowloon, Hong Kong, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
5.Ocean Univ China, Coll Marine Life Sci, Qingdao, Peoples R China
6.Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao, Peoples R China
7.Hong Kong Univ Sci & Technol, Southern Marine Sci & Engn Guangdong, Hong Kong Branch, Kowloon, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Shuwen,Li, Cheng,Cheung, Shunyan,et al. Snapshot of Peptidomics of the Red Tide Forming Species Noctiluca scintillans[J]. FRONTIERS IN MARINE SCIENCE,2020,7:11.
APA Zhang, Shuwen.,Li, Cheng.,Cheung, Shunyan.,Sun, Mingming.,Song, Shuqun.,...&Liu, Hongbin.(2020).Snapshot of Peptidomics of the Red Tide Forming Species Noctiluca scintillans.FRONTIERS IN MARINE SCIENCE,7,11.
MLA Zhang, Shuwen,et al."Snapshot of Peptidomics of the Red Tide Forming Species Noctiluca scintillans".FRONTIERS IN MARINE SCIENCE 7(2020):11.
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