Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Snapshot of Peptidomics of the Red Tide Forming Species Noctiluca scintillans | |
| Zhang, Shuwen1; Li, Cheng2; Cheung, Shunyan3; Sun, Mingming2; Song, Shuqun4; Guo, Wang2; Guo, Cui5,6; Wu, Gongguo2; Liu, Hongbin3,7 | |
| 2020-10-27 | |
| Source Publication | FRONTIERS IN MARINE SCIENCE
 |
| Volume | 7Pages:11 |
| Corresponding Author | Liu, Hongbin(liuhb@ust.hk) |
| Abstract | The objective of this study was to investigate the peptidome of a red tide forming species Noctiluca scintillans based on mass-spectrometry (MS) analysis. N. scintilans cells were collected at the initiation and peak phases of its bloom. Peptides were extracted by ultrafiltration with cutoff of 10 and 3 kDa in acidic condition. Optimal mass spectra were generated after purification with C18 column. MS analysis and peptide identification was undertaken by Linear Trap Quadropole (LTQ) Velos MS/MS instrument. Using this method, 239 and 332 peptide sequences from the samples collected at the initiation and peak phases of N. scintillans bloom were identified, respectively. These sequences were classified and they were affiliated to 68 and 93 groups of precursor proteins, respectively, at the initiation and peak phases, with a compositional dissimilarity of 49.5%. Meanwhile, majority of the peptides were conjectured to come from contents digested by N. scintilans. Thirty-three groups of precursor proteins were detected at both phases. These peptides involved in various intracellular physiological activities, most of them responsible for functions of digestion activities such as oxidoreductase activity, catalytic activity, hydrolase activity and cofactor/ion binding. The compositions of precursor proteins, molecular weights, functions and cell compartments of the obtained peptides were quite different between two bloom stages. This is the first attempt to study peptidomics of N. scintillans, which provide important information on the functional studies of peptidomics in N. scintillans, especially food digestion and dietary protein utilization, as well as their relationship with bloom development. |
| Keyword | Noctiluca scintillans peptidomics mass spectrometry protein food digestion |
| DOI | 10.3389/fmars.2020.569807 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[41906117] ; Guangdong MEPP Fund[GDOE (2019) A23] ; Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[SMSEGL20SC01] ; Research Grants Council of Hong Kong[T21/602/16] ; Research Grants Council of Hong Kong[N_HKUST609/15] ; Research Grants Council of Hong Kong[16128416] |
| WOS Research Area | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| WOS Subject | Environmental Sciences ; Marine & Freshwater Biology |
| WOS ID | WOS:000585733600001 |
| Publisher | FRONTIERS MEDIA SA |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/169112 |
| Collection | 海洋生态与环境科学重点实验室 |
| Corresponding Author | Liu, Hongbin |
| Affiliation | 1.South China Normal Univ, Coll Life Sci, Guangdong Prov Key Lab Healthy & Safe Aquaculture, Guangzhou, Peoples R China 2.Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China 3.Hong Kong Univ Sci & Technol, Dept Ocean Sci, Kowloon, Hong Kong, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 5.Ocean Univ China, Coll Marine Life Sci, Qingdao, Peoples R China 6.Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao, Peoples R China 7.Hong Kong Univ Sci & Technol, Southern Marine Sci & Engn Guangdong, Hong Kong Branch, Kowloon, Hong Kong, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Shuwen,Li, Cheng,Cheung, Shunyan,et al. Snapshot of Peptidomics of the Red Tide Forming Species Noctiluca scintillans[J]. FRONTIERS IN MARINE SCIENCE,2020,7:11. |
| APA | Zhang, Shuwen.,Li, Cheng.,Cheung, Shunyan.,Sun, Mingming.,Song, Shuqun.,...&Liu, Hongbin.(2020).Snapshot of Peptidomics of the Red Tide Forming Species Noctiluca scintillans.FRONTIERS IN MARINE SCIENCE,7,11. |
| MLA | Zhang, Shuwen,et al."Snapshot of Peptidomics of the Red Tide Forming Species Noctiluca scintillans".FRONTIERS IN MARINE SCIENCE 7(2020):11. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| fmars-07-569807.pdf（1710KB） | 期刊论文 | 出版稿 | 延迟开放 | CC BY-NC-SA | View 2023-7-1后可获取 | |
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