Snapshot of Peptidomics of the Red Tide Forming Species Noctiluca scintillans

doi:10.3389/fmars.2020.569807

	Snapshot of Peptidomics of the Red Tide Forming Species Noctiluca scintillans
	Zhang, Shuwen 1; Li, Cheng 2; Cheung, Shunyan 3; Sun, Mingming 2; Song, Shuqun 4; Guo, Wang 2; Guo, Cui 5,6; Wu, Gongguo 2; Liu, Hongbin 3,7
	2020-10-27
发表期刊	FRONTIERS IN MARINE SCIENCE
卷号	7 页码:11
通讯作者	Liu, Hongbin(liuhb@ust.hk)
摘要	The objective of this study was to investigate the peptidome of a red tide forming species Noctiluca scintillans based on mass-spectrometry (MS) analysis. N. scintilans cells were collected at the initiation and peak phases of its bloom. Peptides were extracted by ultrafiltration with cutoff of 10 and 3 kDa in acidic condition. Optimal mass spectra were generated after purification with C18 column. MS analysis and peptide identification was undertaken by Linear Trap Quadropole (LTQ) Velos MS/MS instrument. Using this method, 239 and 332 peptide sequences from the samples collected at the initiation and peak phases of N. scintillans bloom were identified, respectively. These sequences were classified and they were affiliated to 68 and 93 groups of precursor proteins, respectively, at the initiation and peak phases, with a compositional dissimilarity of 49.5%. Meanwhile, majority of the peptides were conjectured to come from contents digested by N. scintilans. Thirty-three groups of precursor proteins were detected at both phases. These peptides involved in various intracellular physiological activities, most of them responsible for functions of digestion activities such as oxidoreductase activity, catalytic activity, hydrolase activity and cofactor/ion binding. The compositions of precursor proteins, molecular weights, functions and cell compartments of the obtained peptides were quite different between two bloom stages. This is the first attempt to study peptidomics of N. scintillans, which provide important information on the functional studies of peptidomics in N. scintillans, especially food digestion and dietary protein utilization, as well as their relationship with bloom development.
关键词	Noctiluca scintillans peptidomics mass spectrometry protein food digestion
DOI	10.3389/fmars.2020.569807
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41906117] ; Guangdong MEPP Fund[GDOE (2019) A23] ; Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[SMSEGL20SC01] ; Research Grants Council of Hong Kong[T21/602/16] ; Research Grants Council of Hong Kong[N_HKUST609/15] ; Research Grants Council of Hong Kong[16128416]
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000585733600001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169112
专题	海洋生态与环境科学重点实验室
通讯作者	Liu, Hongbin
作者单位	1.South China Normal Univ, Coll Life Sci, Guangdong Prov Key Lab Healthy & Safe Aquaculture, Guangzhou, Peoples R China 2.Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China 3.Hong Kong Univ Sci & Technol, Dept Ocean Sci, Kowloon, Hong Kong, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 5.Ocean Univ China, Coll Marine Life Sci, Qingdao, Peoples R China 6.Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao, Peoples R China 7.Hong Kong Univ Sci & Technol, Southern Marine Sci & Engn Guangdong, Hong Kong Branch, Kowloon, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Shuwen,Li, Cheng,Cheung, Shunyan,et al. Snapshot of Peptidomics of the Red Tide Forming Species Noctiluca scintillans[J]. FRONTIERS IN MARINE SCIENCE,2020,7:11.
APA	Zhang, Shuwen.,Li, Cheng.,Cheung, Shunyan.,Sun, Mingming.,Song, Shuqun.,...&Liu, Hongbin.(2020).Snapshot of Peptidomics of the Red Tide Forming Species Noctiluca scintillans.FRONTIERS IN MARINE SCIENCE,7,11.
MLA	Zhang, Shuwen,et al."Snapshot of Peptidomics of the Red Tide Forming Species Noctiluca scintillans".FRONTIERS IN MARINE SCIENCE 7(2020):11.

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