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A method to establish an "immortalized F-2" sporophyte population in the economic brown alga Undaria pinnatifida (Laminariales: Alariaceae)
Shan, Tifeng; Pang, Shaojun1; Wang, Xuemei; Li, Jing; Li, Qianxi; Su, Li; Li, Xiaodong
2020-10-27
Source PublicationJOURNAL OF PHYCOLOGY
ISSN0022-3646
Pages6
Corresponding AuthorPang, Shaojun(sjpang@qdio.ac.cn)
AbstractStudies of quantitative trait loci based on genetic linkage maps require the establishment of a mapping population. Permanent mapping populations are more ideal than temporary ones because they can be used repeatedly. However, there has been no reported permanent sporophyte population of economically important kelp species. Based on the characteristics of the kelp life cycle, we proposed a method to establish, and then constructed experimentally, an "immortalized F-2" (IF2) population of Undaria pinnatifida. Doubled-haploid "female" and "male" sporophytes were obtained through the parthenogenesis of a female gametophyte clone and the selfing of a "monoicous" gametophyte clone (originally male), respectively, and they were used as the parents. The F-1 hybrid line was generated by crossing the female and male gametophytes derived from the respective female and male parents. Full-sibling F-2 gametophyte clones, consisting of 260 females and 260 males, were established from an F-1 hybrid sporophyte. Thirty-five females and 35 males were randomly selected and paired to give rise to an IF2 population containing 35 crossing lines. A parentage analysis using 10 microsatellite markers confirmed the accuracy of the IF2 population and indicated the feasibility of this method. This proposed method may be adapted for use in other kelp species, and thus, it will be useful for genetic studies of kelp.
Keywordbreeding kelp macroalgae permanent population quantitative trait loci seaweed
DOI10.1111/jpy.13066
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41676128] ; Biological Resources Program from Chinese Academy of Sciences[KFJ-BRP-017-27] ; China Agriculture Research System[CARS-50] ; Taishan Scholar Program of Shandong Province ; Foundation for Huiquan Scholar of Institute of Oceanology, Chinese Academy of Sciences
WOS Research AreaPlant Sciences ; Marine & Freshwater Biology
WOS SubjectPlant Sciences ; Marine & Freshwater Biology
WOS IDWOS:000583511200001
PublisherWILEY
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/168978
Collection实验海洋生物学重点实验室
Corresponding AuthorPang, Shaojun
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Shan, Tifeng,Pang, Shaojun,Wang, Xuemei,et al. A method to establish an "immortalized F-2" sporophyte population in the economic brown alga Undaria pinnatifida (Laminariales: Alariaceae)[J]. JOURNAL OF PHYCOLOGY,2020:6.
APA Shan, Tifeng.,Pang, Shaojun.,Wang, Xuemei.,Li, Jing.,Li, Qianxi.,...&Li, Xiaodong.(2020).A method to establish an "immortalized F-2" sporophyte population in the economic brown alga Undaria pinnatifida (Laminariales: Alariaceae).JOURNAL OF PHYCOLOGY,6.
MLA Shan, Tifeng,et al."A method to establish an "immortalized F-2" sporophyte population in the economic brown alga Undaria pinnatifida (Laminariales: Alariaceae)".JOURNAL OF PHYCOLOGY (2020):6.
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