Structural and Functional Insights into Iturin W, a Novel Lipopeptide Produced by the Deep-Sea Bacterium Bacillus sp. Strain wsm-1

doi:10.1128/AEM.01597-20

IOCAS-IR > 实验海洋生物学重点实验室

	Structural and Functional Insights into Iturin W, a Novel Lipopeptide Produced by the Deep-Sea Bacterium Bacillus sp. Strain wsm-1
	Zhou, Shengnan 1; Liu, Ge 2,3,4; Zheng, Rikuan 2,3,4; Sun, Chaomin 2,3,4; Wu, Shimei 1
	2020-11-01
发表期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN	0099-2240
卷号	86 期号:21 页码:12
通讯作者	Wu, Shimei(shimeiwu2016@126.com)
摘要	In the present study, a deep-sea bacterial strain designated Bacillus sp. strain wsm-1 was screened and found to exhibit strong antifungal activity against many plant-pathogenic fungi, and corresponding antifungal agents were thereby purified and determined by tandem mass spectrometry to be two cyclic lipopeptide homologs. These homologs, which were different from any previously reported lipopeptides, were identified to possess identical amino acid sequences of beta-amino fatty acid-Asn-Ser-Asn-Pro-Tyr-Asn-Gln and deduced as two novel lipopeptides designated C-14 iturin W and C-15 iturin W. Electron microscopy observation indicated that both iturin W homologs caused obvious morphological changes and serious disruption of plasma membrane toward fungal cells, while C-15 iturin W exhibited more serious cell damages than C-14 iturin W did, which was well consistent with the results of the antifungal activity assays. To improve the yield and antifungal activity of iturin W, the effects of different carbon and nitrogen sources and amino acids on production of C-14 iturin W and C-15 iturin W were investigated. The results indicated that supplements of most of the detected carbon and nitrogen sources could increase the yield of C-14 iturin W, but inhibit the yield of C-15 iturin W, while supplements of tryptone and most of the detected amino acids could increase the yield of both C-14 iturin W and C-15 iturin W. IMPORTANCE Plant disease caused by pathogenic fungi is one of the most devastating diseases, which affects the food safety of the whole world to a great extent. Biological control of plant diseases by microbial natural products is more desirable than traditional chemical control. In this study, we discovered a novel lipopeptide, iturin W, with promising prospects in biological control of plant diseases. Moreover, the effects of different carbon and nitrogen sources and amino acids on production of C-14 iturin W and C-15 iturin W would provide a reasonable basis for the optimization of the fermentation process of lipopeptides. Notably, the structure of iturin W was different from that of any previously reported lipopeptide, suggesting that deep-sea microorganisms might produce many novel natural products and have significant potential in the development of biological products in the future.
关键词	Bacillus biocontrol antifungal lipopeptide iturin
DOI	10.1128/AEM.01597-20
收录类别	SCI
语种	英语
资助项目	National Key R and D Program of China[2018YFC0310800] ; China Ocean Mineral Resources R&D Association Grant[DY135-B2-14] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051] ; Qingdao Innovation Leadership Program[18-1-2-7-zhc]
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000582928500012
出版者	AMER SOC MICROBIOLOGY
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168960
专题	实验海洋生物学重点实验室
通讯作者	Wu, Shimei
作者单位	1.Qingdao Univ, Coll Life Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Shengnan,Liu, Ge,Zheng, Rikuan,et al. Structural and Functional Insights into Iturin W, a Novel Lipopeptide Produced by the Deep-Sea Bacterium Bacillus sp. Strain wsm-1[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2020,86(21):12.
APA	Zhou, Shengnan,Liu, Ge,Zheng, Rikuan,Sun, Chaomin,&Wu, Shimei.(2020).Structural and Functional Insights into Iturin W, a Novel Lipopeptide Produced by the Deep-Sea Bacterium Bacillus sp. Strain wsm-1.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,86(21),12.
MLA	Zhou, Shengnan,et al."Structural and Functional Insights into Iturin W, a Novel Lipopeptide Produced by the Deep-Sea Bacterium Bacillus sp. Strain wsm-1".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 86.21(2020):12.

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