The carbonate concentration mechanism ofPyropia yezoensis(Rhodophyta): evidence from transcriptomics and biochemical data

doi:10.1186/s12870-020-02629-4

IOCAS-IR > 实验海洋生物学重点实验室

	The carbonate concentration mechanism ofPyropia yezoensis(Rhodophyta): evidence from transcriptomics and biochemical data
	Zhang, Baoyu 1,2; Xie, Xiujun 1,2; Liu, Xuehua 1,2; He, Linwen 1,2; Sun, Yuanyuan1,2 ; Wang, Guangce 1,2
	2020-09-15
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	20 期号:1 页码:12
通讯作者	Wang, Guangce(gcwang@qdio.ac.cn)
摘要	Background Pyropia yezoensis(Rhodophyta) is widely cultivated in East Asia and plays important economic, ecological and research roles. Although inorganic carbon utilization ofP. yezoensishas been investigated from a physiological aspect, the carbon concentration mechanism (CCM) ofP. yezoensisremains unclear. To explore the CCM ofP. yezoensis, especially during its different life stages, we tracked changes in the transcriptome, photosynthetic efficiency and in key enzyme activities under different inorganic carbon concentrations. Results Photosynthetic efficiency demonstrated that sporophytes were more sensitive to low carbon (LC) than gametophytes, with increased photosynthesis rate during both life stages under high carbon (HC) compared to normal carbon (NC) conditions. The amount of starch and number of plastoglobuli in cells corresponded with the growth reaction to different inorganic carbon (Ci) concentrations. We constructed 18 cDNA libraries from 18 samples (three biological replicates per Ci treatment at two life cycles stages) and sequenced these using the Illumina platform. De novo assembly generated 182,564 unigenes, including approximately 275 unigenes related to CCM. Most genes encoding internal carbonic anhydrase (CA) and bicarbonate transporters involved in the biophysical CCM pathway were induced under LC in comparison with NC, with transcript abundance of somePyCAs in gametophytes typically higher than that in sporophytes. We identified all key genes participating in the C4 pathway and showed that their RNA abundances changed with varying Ci conditions. High decarboxylating activity of PEPCKase and low PEPCase activity were observed inP. yezoensis. Activities of other key enzymes involved in the C4-like pathway were higher under HC than under the other two conditions. Pyruvate carboxylase (PYC) showed higher carboxylation activity than PEPC under these Ci conditions. Isocitrate lyase (ICL) showed high activity, but the activity of malate synthase (MS) was very low. Conclusion We elucidated the CCM ofP. yezoensisfrom transcriptome and enzyme activity levels. All results indicated at least two types of CCM inP. yezoensis, one involving CA and an anion exchanger (transporter), and a second, C4-like pathway belonging to the PEPCK subtype. PYC may play the main carboxylation role in this C4-like pathway, which functions in both the sporophyte and gametophyte life cycles.
关键词	Carbon concentrating mechanism Enzyme activity Pyropia yezoensis Photosynthetic efficiency Transcriptome
DOI	10.1186/s12870-020-02629-4
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41876160] ; National Natural Science Foundation of China[41876163] ; National key R&D program of China[2016YFC1400602-03] ; Key R&D program of Yantai City[2018SFBF069]
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000573285100001
出版者	BMC
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168932
专题	实验海洋生物学重点实验室
通讯作者	Wang, Guangce
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Baoyu,Xie, Xiujun,Liu, Xuehua,et al. The carbonate concentration mechanism ofPyropia yezoensis(Rhodophyta): evidence from transcriptomics and biochemical data[J]. BMC PLANT BIOLOGY,2020,20(1):12.
APA	Zhang, Baoyu,Xie, Xiujun,Liu, Xuehua,He, Linwen,Sun, Yuanyuan,&Wang, Guangce.(2020).The carbonate concentration mechanism ofPyropia yezoensis(Rhodophyta): evidence from transcriptomics and biochemical data.BMC PLANT BIOLOGY,20(1),12.
MLA	Zhang, Baoyu,et al."The carbonate concentration mechanism ofPyropia yezoensis(Rhodophyta): evidence from transcriptomics and biochemical data".BMC PLANT BIOLOGY 20.1(2020):12.

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